Zein active peptide and preparation technique thereof

Abstract

The invention provides a zein bioactive peptide and a preparation technology thereof. The technology comprises the steps that: corn protein powder is crushed into fine powder of 80 to 100 meshes; water which is 8 to 15 times of the weight of corn protein powder is added so as to form slurry; autoclaving is carried out at the temperature of 121 DEG C for 10 to 20 min, the mixture is cooled down to 45 plus or minus 1 DEG C; neutral protease which is 0.1 to 0.5 time of the weight of the corn protein is added into the slurry, then zymohydrolysis is carried out at the temperature of 45 plus or minus 1 DEG C for 1.5 to 3.0 hours; the autoclaving is carried out at the temperature of 121 DEG C for 10 min, then the mixture is cooled down to 45 plus or minus 1 DEG C; an Aspergillus oryzae strain which is 10 percent of the total weight of the slurry is accessed aseptically at the temperature of 35 plus or minus 1 DEG C; and the mixture is put into a shaker for fermentation for 10 to 12 hours so as to obtain the zein bioactive peptide. The preparation technology of the invention has the advantages that: the reaction process is steady and easier to be controlled; the final product has low content of impurity and high content of amino acid, with good mouthfeel and no abnormal taste of bitter and harsh, etc., meanwhile, the production cost is relatively low.

Description

technical field

[0001] The invention relates to zein deep-processing products, in particular provides a zein active peptide, and also discloses its preparation process, belonging to the technical field of edible protein preparation. Background technique:

[0002] The zein active peptide involved in the present invention is a deep-processing product of zein, which has good solubility and fluidity, fast absorption in the human body, and high utilization rate; it also has anti-hypertension, anti-cholesterol, anti-thrombosis, and improves lipid Mass metabolism, eliminate excess fat, promote the recovery of red blood cells, help recover from fatigue, and promote the absorption of calcium, phosphorus and other trace elements. Corn peptide also has free radical scavenging, anti-oxidation, enhances phagocytic and B cell activity, enhances immune function, anti-cancer, anti-aging, protects the liver, prevents the occurrence of senile dementia, senile osteoporosis and gallstones, help...

Examples

Embodiment 1

[0022] 1. Crush zein into 80-100 mesh fine powder, add 8 times the weight of water to make a slurry, autoclave at 121°C for 10 minutes, and cool to 45±1°C.

[0023] 2. Add 0.1 times the weight of zein neutral protease to the slurry, and enzymatically hydrolyze for 1.5 hours at a temperature of 45±1°C.

[0024] 3. After autoclaving at 121°C for 10 minutes, cool to 45±1°C.

[0025] 4. Aseptically inoculate Aspergillus oryzae strains into the slurry by 10% of the total amount of the slurry, at a temperature of 35±1° C., put into a shaker for fermentation until 10 hours.

[0026] Analyze and test once per hour to measure x-ammonia nitrogen and peptide nitrogen. About 10% of x-ammonia nitrogen and 70-75% of the total nitrogen are the targets. After reaching the targets, sterilize at 121°C After 15 minutes, centrifuge (or filter), discard the precipitate, evaporate and concentrate the supernatant to 40BX, and then spray-dry (or freeze-dry) to make a finished product.

Embodiment 2

[0028] 1. Crush zein into 80-100 mesh fine powder, add 12 times the weight of water to make a slurry, autoclave at 121°C for 15 minutes, and cool to 45±1°C.

[0029] 2. Add 0.3 times the weight of zein neutral protease to the slurry, and enzymatically hydrolyze for 2 hours at a temperature of 45±1°C.

[0030] 3. After autoclaving at 121°C for 10 minutes, cool to 45±1°C.

[0031] 4. Aseptically inoculate Aspergillus oryzae strains into the slurry by 10% of the total amount of the slurry, at a temperature of 35±1° C., put into a shaker for fermentation until 11 hours.

[0032] Analyze and test once per hour to measure x-ammonia nitrogen and peptide nitrogen. About 10% of x-ammonia nitrogen and 70-75% of the total nitrogen are the targets. After reaching the targets, sterilize at 121°C After 15 minutes, centrifuge (or filter), discard the precipitate, evaporate and concentrate the supernatant to 40BX, and then spray-dry (or freeze-dry) to make a finished product.

Embodiment 3

[0034] 1. Crush zein into 80-100 mesh fine powder, add 15 times the weight of water to make a slurry, autoclave at 121°C for 20 minutes, and cool to 45±1°C.

[0035] 2. Add 0.5 times the weight of zein neutral protease to the slurry, and enzymatically hydrolyze for 3 hours at a temperature of 45±1°C.

[0036] 3. After autoclaving at 121°C for 10 minutes, cool to 45±1°C.

[0037] 4. Aseptically inoculate Aspergillus oryzae strains into the slurry by 10% of the total amount of the slurry, at a temperature of 35±1° C., put into a shaker for fermentation until 12 hours.

[0038] Analyze and test once per hour to measure x-ammonia nitrogen and peptide nitrogen. About 10% of x-ammonia nitrogen and 70-75% of the total nitrogen are the targets. After reaching the targets, sterilize at 121°C After 15 minutes, centrifuge (or filter), discard the precipitate, evaporate and concentrate the supernatant to 40BX, and then spray-dry (or freeze-dry) to make a finished product.

technical field

[0001] The invention relates to zein deep-processing products, in particular provides a zein active peptide, and also discloses its preparation process, belonging to the technical field of edible protein preparation. Background technique:

[0002] The zein active peptide involved in the present invention is a deep-processing product of zein, which has good solubility and fluidity, fast absorption in the human body, and high utilization rate; it also has anti-hypertension, anti-cholesterol, anti-thrombosis, and improves lipid Mass metabolism, eliminate excess fat, promote the recovery of red blood cells, help recover from fatigue, and promote the absorption of calcium, phosphorus and other trace elements. Corn peptide also has free radical scavenging, anti-oxidation, enhances phagocytic and B cell activity, enhances immune function, anti-cancer, anti-aging, protects the liver, prevents the occurrence of senile dementia, senile osteoporosis and gallstones, help...

Examples

Embodiment 1

[0022] 1. Crush zein into 80-100 mesh fine powder, add 8 times the weight of water to make a slurry, autoclave at 121°C for 10 minutes, and cool to 45±1°C.

[0023] 2. Add 0.1 times the weight of zein neutral protease to the slurry, and enzymatically hydrolyze for 1.5 hours at a temperature of 45±1°C.

[0024] 3. After autoclaving at 121°C for 10 minutes, cool to 45±1°C.

[0025] 4. Aseptically inoculate Aspergillus oryzae strains into the slurry by 10% of the total amount of the slurry, at a temperature of 35±1° C., put into a shaker for fermentation until 10 hours.

[0026] Analyze and test once per hour to measure x-ammonia nitrogen and peptide nitrogen. About 10% of x-ammonia nitrogen and 70-75% of the total nitrogen are the targets. After reaching the targets, sterilize at 121°C After 15 minutes, centrifuge (or filter), discard the precipitate, evaporate and concentrate the supernatant to 40BX, and then spray-dry (or freeze-dry) to make a finished product.

Embodiment 2

[0028] 1. Crush zein into 80-100 mesh fine powder, add 12 times the weight of water to make a slurry, autoclave at 121°C for 15 minutes, and cool to 45±1°C.

[0029] 2. Add 0.3 times the weight of zein neutral protease to the slurry, and enzymatically hydrolyze for 2 hours at a temperature of 45±1°C.

[0030] 3. After autoclaving at 121°C for 10 minutes, cool to 45±1°C.

[0031] 4. Aseptically inoculate Aspergillus oryzae strains into the slurry by 10% of the total amount of the slurry, at a temperature of 35±1° C., put into a shaker for fermentation until 11 hours.

[0032] Analyze and test once per hour to measure x-ammonia nitrogen and peptide nitrogen. About 10% of x-ammonia nitrogen and 70-75% of the total nitrogen are the targets. After reaching the targets, sterilize at 121°C After 15 minutes, centrifuge (or filter), discard the precipitate, evaporate and concentrate the supernatant to 40BX, and then spray-dry (or freeze-dry) to make a finished product.

Embodiment 3

[0034] 1. Crush zein into 80-100 mesh fine powder, add 15 times the weight of water to make a slurry, autoclave at 121°C for 20 minutes, and cool to 45±1°C.

[0035] 2. Add 0.5 times the weight of zein neutral protease to the slurry, and enzymatically hydrolyze for 3 hours at a temperature of 45±1°C.

[0036] 3. After autoclaving at 121°C for 10 minutes, cool to 45±1°C.

[0037] 4. Aseptically inoculate Aspergillus oryzae strains into the slurry by 10% of the total amount of the slurry, at a temperature of 35±1° C., put into a shaker for fermentation until 12 hours.

[0038] Analyze and test once per hour to measure x-ammonia nitrogen and peptide nitrogen. About 10% of x-ammonia nitrogen and 70-75% of the total nitrogen are the targets. After reaching the targets, sterilize at 121°C After 15 minutes, centrifuge (or filter), discard the precipitate, evaporate and concentrate the supernatant to 40BX, and then spray-dry (or freeze-dry) to make a finished product.

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