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Nasopharyngeal carcinoma early stage blood serum special protein and uses thereof

A nasopharyngeal carcinoma, serum technology, applied in the direction of measuring devices, instruments, material analysis through electromagnetic means, etc.

Inactive Publication Date: 2009-01-28
福建省肿瘤医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

No serum markers or marker proteins related to nasopharyngeal carcinoma have been found so far, so it is of great significance to use mass spectrometry to analyze the serum of nasopharyngeal carcinoma patients to obtain specific tumor markers

Method used

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  • Nasopharyngeal carcinoma early stage blood serum special protein and uses thereof
  • Nasopharyngeal carcinoma early stage blood serum special protein and uses thereof
  • Nasopharyngeal carcinoma early stage blood serum special protein and uses thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0035] Determination of Serum Specific Proteins in Early Nasopharyngeal Carcinoma

[0036] (1) Experimental samples

[0037] 1. Serum of patients with nasopharyngeal carcinoma: 156 cases, 96 males and 60 females, with a median age of 40 years old and diagnosed by clinical pathology without radiotherapy and chemotherapy.

[0038] 2. Control serum: 159 cases, 98 males and 61 females, with a median age of 45 years, were taken from healthy volunteers.

[0039] (2) Experimental materials

[0040] 1. Reagents and chips: urea, sodium acetate, acetonitrile, trifluoroacetic acid, DTT, and CHAPS were purchased from Sigma, USA; Sinapinic acid (SPA) and weak cation exchange chip (weak cation exchanger) CM10 were purchased from Ciphergen Biosytems, Inc Fremont , USA company.

[0041] 2. Protein chip reader: protein chip reader (PBSII-C type, Ciphergen Biosystems, Inc. Fremont, USA), according to the principle of laser desorption ion time-of-flight, can detect peptides as small as 1000Da...

Embodiment 2

[0073] A specific example of the classification model of serum protein spectrum related to nasopharyngeal carcinoma can assist clinical analysis of the risk of nasopharyngeal carcinoma in patients.

[0074] 1: Patient Zhang Moumou, female, 39 years old, medical record number 76756, the patient came to the Cancer Hospital in November 2006 due to the discovery of a tumor in the right neck in February 2006, the EB virus test was negative, and the conventional tumor marker tests were all negative . Serum protein flight mass spectrometry was performed after admission, and the results showed ( Figure 8-10 Shown): the protein peak intensity of mass-to-nucleus ratio of 4182Da is 3.2, the peak intensity of protein of mass-to-nucleus ratio of 4298Da is 2.8, the peak intensity of protein of mass-to-nucleus ratio of 6450Da is 14.3, and the peak intensity of protein of mass-to-nucleus ratio of 13755Da is 5.9. The serum protein model judged to be consistent with the performance of nasopha...

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Abstract

The invention discloses an early nasopharyngeal carcinoma serum specific protein and application thereof, belonging to the field of biological detection technique. The invention uses an SELDI-TOF-MS technique, establishes the fingerprint of serum protein between the nasopharyngeal carcinoma patient and healthy contrasting person, carries out the variation analysis and searches new protein markers, thus carrying out early checking and detection on the nasopharyngeal carcinoma. As the SELDI-TOF-MS technique is applied to the invention, mass spectrometric detection and analysis are carried out on the serum of the peripheral blood of nasopharyngeal carcinoma patients from the point of view of proteomics, thus establishing effective nasopharyngeal carcinoma serum protein fingerprint which is used for the risk evaluation method used when the nasopharyngeal carcinoma is detected outside the body. The method can be used as the auxiliary checking mean of nasopharyngeal carcinoma non-invasion.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a tumor marker-nasopharyngeal carcinoma early serum specific protein, a method for detecting the risk of nasopharyngeal carcinoma in vitro and its application. Background technique [0002] Nasopharyngeal carcinoma is a malignant tumor that originates in the epithelium of nasopharyngeal mucosa. It is the most common cancer in otolaryngology. China's Fujian, Guangdong and other provinces are areas with a high incidence of nasopharyngeal carcinoma, and the incidence rate can reach 30 / 100,000 to 50 / 100,000. The main symptoms are hearing loss, foreign body sensation in the pharynx, pharyngitis, glossitis, gingivitis, dysphagia, and abnormal breathing. [0003] Clinical studies have found that the clinical stage of nasopharyngeal carcinoma is an important factor affecting the treatment effect. The 5-year survival rate (89.7%, 75.9%) of early stage nasopharyngeal carcinom...

Claims

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Application Information

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IPC IPC(8): G01N33/483G01N27/64
Inventor 潘建基叶韵斌刘枋李建成陈强陈慧菁倪晓雷郭福榕
Owner 福建省肿瘤医院
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