Sequencing reaction small chamber, gene sequencing reaction bench and gene sequencing system

Abstract

The invention provides a sequencing reaction cabinet, used for carrying out the sequencing reaction between DNA fragments and a reagent; the sequencing reaction cabinet comprises a reaction cavity, on the inner wall at one side of which is fixedly provided with a plurality of DNA fragments; a reagent inlet and a reagent outlet which are respectively arranged at the two ends of the inner wall at the other side of the reaction cavity and are respectively used for the reagent to flow into and out of the reaction cavity. In the sequencing reaction cabinet, a plurality of DNA fragments are fixed in the reaction cavity with a small capacity as a short label array for sequencing to lead the sequencing reaction to be carried out in the reagent without a diffusion barrier, thus greatly improving the accessibility between the reagent and the DNA fragments and further shortening the reaction time; the requirements to the concentration and the dosage of the reagent is lower, thus consuming less reagent and reducing the sequencing cost. While a plurality of DNA fragments are simultaneously fixed in the reaction cabinet to provide a platform for realizing the parallel reaction of a plurality of fragments. The sequencing reaction cabinet used for carrying out sequencing reaction between DNA fragments and a reagent realizes the automatization of high pass sequencing and can realizes fast sequencing reaction.

Description

technical field [0001] The invention relates to the field of nucleotides, in particular to a sequencing reaction chamber, a gene sequencing reaction table and a gene sequencing system. Background technique [0002] Existing traditional sequencing technologies generally use gel electrophoresis to distinguish different base lengths. [0003] An existing technique is to add dideoxynucleoside triphosphate (ddNTP) during DNA polymerization. Dideoxynucleoside triphosphate lacks a hydroxyl group at the 3' position of deoxyribose, so it cannot form a phosphodiester bond with subsequent dNTP. In the presence of ddCTP, dCTP, and three other dNTPs, primers, template, and DNA polymerase are incubated together to form a DNA that all has the same 5'-primer end and ends with a ddC residue at the 3' end. A series of mixtures of fragments of different lengths. For the four bases, each component in each group of products (select any of the four bases) will be separated according to their cha...

AI Technical Summary

Problems solved by technology

Since the probe can only be synthesized in advance according to the known sequence, this prior art is a closed system, which cannot determine the unknown sequence, and can only analyze whether the sample contains known gene fragments

This existing technology uses the synthesis of multiple probes on the same substrate, which has the characteristics of high throughput, but because the hybridization reaction is not independent, the interaction between the sequences is relatively large, so the accuracy of the data is not enough

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