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Curcumin glutin microsphere and preparation thereof

A technology of curcumin gelatin and preparation process, which is applied in the field of curcumin gelatin microspheres and its preparation, can solve the problems of no curcumin microspheres, etc., and achieve the effects of good fluidity, good dispersibility, and good stability

Inactive Publication Date: 2010-12-22
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there are no patent literature reports on curcumin microspheres and their preparation process

Method used

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  • Curcumin glutin microsphere and preparation thereof
  • Curcumin glutin microsphere and preparation thereof
  • Curcumin glutin microsphere and preparation thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0019] Embodiment 1: prepare curcumin gelatin microspheres, the steps are as follows:

[0020] With liquid paraffin as the oil phase and Span80 as the emulsifier, slowly add 2 mL of curcumin-containing gelatin aqueous solution to 8 mL of liquid paraffin containing 2% Span80 at a temperature of 55 °C under 800 r / min electromagnetic stirring , wherein the mass concentration of gelatin is 20%, and the mass ratio of curcumin to gelatin is 1:20; stir for 30min to form a W / O emulsion, then cool down to 20°C, add 0.4mL of 25% glutaraldehyde solution, ice bath, Keep the temperature at 5-8°C, stir and solidify for 30 minutes, then put it in a refrigerator at 4-8°C to continue solidifying for 24 hours; pour out the upper oil phase, add isopropanol to dehydrate, filter with suction, and rinse several times with ether and isopropanol alternately , to obtain dry powder microspheres. It was determined that the drug loading was 2.22%, and the encapsulation efficiency was 27.50%.

Embodiment 2

[0021] Embodiment 2: prepare curcumin gelatin microspheres, the steps are as follows:

[0022] With liquid paraffin as the oil phase and Span80 as the emulsifier, at a temperature of 55°C, slowly add 2 mL of curcumin-containing gelatin aqueous solution to 8 mL of 1.5% Span80-containing liquid paraffin at a temperature of 55°C under 1000 r / min electromagnetic stirring , wherein the mass concentration of gelatin is 15%, and the mass ratio of curcumin to gelatin is 15:200; stir for 40min to form a W / O emulsion, then cool down to 20°C, add 0.4mL of 25% glutaraldehyde solution, ice bath, Keep the temperature at 5-8°C, stir and solidify for 25 minutes, and then put it in a refrigerator at 4-8°C to continue solidifying for 23.5 hours; pour the upper oil phase, add isopropanol to dehydrate, filter with suction, and rinse with ether and isopropanol alternately for several times Once, dry powdered microspheres were obtained. It was determined that the drug loading was 4.44%, and the en...

Embodiment 3

[0023] Embodiment 3: prepare curcumin gelatin microspheres, the steps are as follows:

[0024] With liquid paraffin as the oil phase, Span80 as the emulsifier, at a temperature of 55°C, slowly add 2mL of aqueous gelatin solution containing curcumin to 8mL of liquid paraffin containing 1% Span80 at a temperature of 55°C under electromagnetic stirring, wherein, The mass concentration of gelatin is 10%, and the mass ratio of curcumin and gelatin is 1:10; stir to form a W / O emulsion, then cool down to 20°C, add 0.4mL of 25% glutaraldehyde solution, and keep the temperature at 5 Stir and solidify at ~8°C for 35 minutes, then put it in a refrigerator at 4-8°C to continue curing for 24.5 hours; pour the upper oil phase, add isopropanol to dehydrate, filter with suction, rinse several times with ether and isopropanol alternately, and dry Powdered microspheres. It was determined that the drug loading was 7.6%, and the encapsulation efficiency was 71.50%.

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Abstract

The invention discloses a curcumin gelatin microsphere, wherein the mass percentage of curcumin in the microsphere is between 2.2 and 7.6, and the balance being gelatin. The method for preparing the curcumin gelatin microsphere comprises the following steps: adding a gelatin aqueous solution containing the curcumin into liquid paraffin containing Span80 under electromagnetic stirring, stirring the mixture to form a W / O emulsion, and then reducing the temperature to 20 DEG C, adding glutaraldehyde solution into the mixture, performing ice bath, keeping the temperature between 5 and 8 DEG C, mixing and curing the mixture, and then putting the mixture in a refrigerator to continue the curing for 24 hours; and dumping the oil phase on the upper layer, adding isopropyl alcohol into the mixturefor dehydration, performing suction filtration on the mixture, and rinsing the mixture with ether and the isopropyl alcohol rinse by turns for a few times to obtain a dry powdered microsphere. The microsphere has pale yellow powdered appearance, is spherical, has a smooth surface, narrow particle size distribution and good dispersity, is not stuck or hardly stuck, and has good mobility. The curcumin gelatin microsphere is prepared by using an inexpensive biodegradable gelatin as a carrier material through an emulsification crosslinking method, thereby having the advantages of simple process, low cost, convenient operation, high encapsulation efficiency, good stability and the like.

Description

technical field [0001] The invention relates to a curcumin gelatin microsphere and a preparation process thereof. Background technique [0002] Curcumin (C for short) is a natural active ingredient extracted from plants of the genus Curcuma (curcuma L.), which has a wide range of preventive and therapeutic effects on tumors, and has little toxic and side effects. In vitro studies have shown that curcumin has inhibitory effects on the production, proliferation and metastasis of various tumor cells, especially for human lung cancer cells, not only can make intracellular Ca 2+ , The cAMP concentration increases, successfully induces its apoptosis, and can make the lung cancer cells with anti-apoptotic characteristics undergo apoptotic apoptosis, which is of great significance for the treatment of malignant metastases. However, its solubility in water is small, its stability is poor, its oral absorption is low, and its bioavailability is low, which greatly limits its applicatio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/12A61K47/42A61K9/14A61P35/00
Inventor 曹丰亮翟光喜唐剑杨静蒋玮
Owner SHANDONG UNIV
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