Method for preparing lyophilized hepatitis A attenuated live vaccine using cell factory

Abstract

The invention discloses a method for preparing a freeze-dried live attenuated hepatitis A vaccine by utilizing a cell factory. The method has good process controllability, is uneasy to pollute environment, is suitable for industrialized and mass production, improves the utilization rate of a culture space by more than 3 times, ensures that the growth density of cells reaches between 4.0x10<6> and 5.0x10<6> cells per ml, assures the multiplication space of viruses, and also effectively solves the problem of cell residue after the cell factory digests through methods such as sterile washing, centrifugal treatment and so on, so as to continuously use the cell factory for cell culture and improve the reuse rate of the cell factory.

Description

Technical field:

[0001] The invention relates to a method for preparing a freeze-dried live attenuated hepatitis A vaccine, in particular discloses a method for preparing a freeze-dried live attenuated hepatitis A vaccine by using a cell factory, and belongs to the technical field of biopharmaceuticals. Background technique:

[0002] The key to the preparation of traditional viral vaccines is to provide the virus with a suitable cell matrix for its proliferation, and the growth method of adherent culture is usually adopted. Adherent culture systems mainly include spinner bottles, hollow fibers, glass beads, and microcarrier systems. Roller bottle culture is generally used for the transition from small-scale culture to large-scale culture, or as a preparation method for inoculating cells in bioreactors. It is characterized by simple structure, low investment, mature technology, good repeatability, easy expansion, etc., but also has disadvantages such as low cell growth densi...