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Method for method for breeding high yield bacterial strain of zuelaemycin producing actinomycetes strain by complex mutation

A technology of compound mutagenesis and seramectin, which is applied in the directions of biochemical equipment and methods, microorganisms, and mutant preparation, etc., can solve unseen problems, and achieve the effect of simple method, easy method and improved antagonistic performance.

Inactive Publication Date: 2009-04-15
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are no relevant patents and literature reports on the application of ultraviolet rays and lithium chloride compound mutagenesis to the selection and breeding of relamectin-producing bacteria.

Method used

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  • Method for method for breeding high yield bacterial strain of zuelaemycin producing actinomycetes strain by complex mutation
  • Method for method for breeding high yield bacterial strain of zuelaemycin producing actinomycetes strain by complex mutation
  • Method for method for breeding high yield bacterial strain of zuelaemycin producing actinomycetes strain by complex mutation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Prepare PDA and fermentation medium to prepare for the next experiment.

[0040] Preparation of spore suspension preparation: Wash the spores of the slant strain cultured for 7 days with 1 ml sterile normal saline and gently scrape the surface of the slant with an inoculation loop. Bed at 200r / min, 28°C, culture for 2h to fully disperse and activate the spores, then filter the mycelium with sterile absorbent cotton, and dilute the spore suspension to 10 with sterile water 8per mL, that is, to make the spore suspension of the starting strain for mutagenesis.

[0041] 3. Mutagenesis

[0042] a: Ultraviolet treatment: the operation is carried out under red light, and the ultraviolet lamp is turned on 0.5h in advance to stabilize the light source. Take 6mL of the spore suspension and place it in a 9cm-diameter stirrer plate. After irradiating for 1min, open the lid and turn on the magnetic stirrer. The distance from the 15W UV lamp is 30cm, and the irradiation time is 15s...

Embodiment 2

[0052] 1. Prepare Gaoshi No. 1 (antagonistic screening) and fermentation medium to prepare for the next experiment.

[0053] 2. Preparation of spore suspension preparation: wash the spores with 5ml sterile saline and inoculate the slant bacterial strain cultivated for 6 days

[0054] Scrape the surface of the inclined surface lightly, add the bacterial suspension to the sterile saline containing the glass strain, put it in a shaker at 200r / min, 28°C, and incubate for 6 hours to fully disperse and activate the spores, and then filter them out with sterile absorbent cotton Mycelia, the spore suspension was diluted to 10 with sterile water 6 per mL, that is, to make the spore suspension of the starting strain for mutagenesis.

[0055] 3. Mutagenesis

[0056] a: Ultraviolet treatment: the operation is carried out under red light, and the ultraviolet lamp is turned on 0.5h in advance to stabilize the light source. Take 6mL of the spore suspension and place it in a 9cm-diameter s...

Embodiment 3

[0066] 1. Prepare Gaoshi No. 1 (antagonistic screening) and fermentation medium to prepare for the next experiment.

[0067] 2. Preparation of spore suspension preparation: wash the spores with 5ml sterile saline and inoculate the slant bacterial strain cultivated for 6 days

[0068] Scrape the surface of the inclined plane lightly, add the bacterial suspension to the sterile water containing the glass strain, put it in a shaker at 150r / min, 30°C, and incubate for 4 hours to fully disperse and activate the spores, and then filter the mycelium with sterile absorbent cotton body, the spore suspension was diluted to 10 with sterile water 6 per mL, that is, to make the spore suspension of the starting strain for mutagenesis.

[0069] 3. Mutagenesis

[0070] a: Ultraviolet treatment: the operation is carried out under red light. Take 10mL of spore suspension and place it in a 9cm diameter plate with a stirrer, turn on the magnetic stirrer, and place it at a distance of 20cm from ...

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Abstract

The invention relates to a method for breeding a superior strain of a zuelaemycin producing actinomycete through compound mutation. The method includes the following processes: slant pores are selected and prepared into a pore suspension liquid by using sterile water under room temperature; the pore suspension liquid is arranged into a plate and a magnetic stirrer is opened, then the pore suspension liquid is irradiated under an uviol lamp for mutagenesis; the pore suspension liquid after mutagenesis is diluted and then coated on a PDA medium which contains lithium chloride; then a mutant strain with the yield level higher than a starting strain is obtained; a pure prescreened strain is selected and switched into a liquid fermentation medium; and then the superior strains with stable hereditary characteristics are selected. The method has the advantages that the mutation device adopted is simple; the method is easy to be carried out; the operation is safe; the compound mutation effect is better than the processing effect of one single mutagenic agent; and compared with the starting strain, the antagonistic property of the mutation strain obtained through the method is improved by 2.5 to 5.0 times.

Description

1. Technical field: [0001] The invention belongs to the technical field of microbial mutation breeding, and in particular relates to a method for breeding high-yielding strains through compound mutagenesis of rilamectin-producing bacteria, which uses ultraviolet light and lithium chloride compound mutagenesis to breed high-yielding strains of rilamectin , followed by the method can effectively improve the yield of relamectin. 2. Background technology: [0002] Since the advent of organochlorine and organomercury pesticides in the 1940s, people have mainly relied on chemical pesticides as a modern weapon in the fight against agricultural pests, diseases and weeds. However, with the rapid increase in the variety and output of chemical pesticides, the scope of application is expanding day by day, and the worldwide public nuisance caused by pesticide residue pollution has aroused people's worries. With the enhancement of human's awareness of environmental protection, bio-pestic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/01C12N13/00
Inventor 安德荣苟丽霞
Owner NORTHWEST A & F UNIV
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