Corn peptide beverage for sobering-up and preparation method thereof
A zein peptide, sobering technology, applied in food preparation, pharmaceutical formulations, plant raw materials, etc., can solve the problems of increasing economic costs, increasing energy consumption, numerous steps, etc., and achieve the effects of low cost, elimination of peculiar smell, and easy separation of products
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Embodiment 1
[0030]The zein peptide of this example is prepared according to the following steps: (1) corn yellow powder is ultrafinely pulverized to 0.1mm (through a 150-mesh sieve), dispersed in an aqueous solution at a weight ratio of corn yellow powder to water of 1:4, and placed in In an airtight pressurized container, adjust the pressure to be 130kpa for 2h, then cool to room temperature; (2) add the corn yellow powder that is pressurized into the reactor, and control the protein mass concentration at 1%; (3) Add the enzyme at a weight ratio of 0.1:1 from the immobilized protease derived from Bacillus amyloliquefaciens to zein protein, and react at a temperature of 25°C, a shaking speed of 50 rpm, and a constant pH at normal pressure. When the DH When the value reaches 20%, the reaction is terminated; (4) the reaction is terminated by filtering the enzyme, the remaining reaction solution is centrifuged at a high speed (10000 rpm) for 30min, and the supernatant is taken; (5) the supern...
Embodiment 2
[0034] The corn sobering peptide of this example is prepared according to the following steps: (1) corn yellow powder is ultrafinely pulverized to 0.04 mm (through a 400 mesh sieve), dispersed in an aqueous solution at a weight ratio of corn yellow powder to water of 1:10, and placed in In an airtight pressurized container, adjust the pressure to be 320kpa for 20 minutes, then cool to room temperature; (2) add the corn yellow powder that is pressurized into the reactor, and control the protein mass concentration at 25%; (3) Add the enzyme according to the weight ratio of immobilized protease derived from animal pancreas (pancreas) to zein protein in a ratio of 3:1, react at a temperature of 35°C, a shaking speed of 150rpm, and a constant pH at normal pressure. When the DH When the value reaches 25%, the reaction is terminated; (4) the reaction is terminated by filtering the enzyme, the remaining reaction solution is centrifuged at a high speed (12000 rpm) for 20min, and the sup...
Embodiment 3
[0036] The corn hangover peptide of this embodiment is prepared according to the following steps: (1) corn yellow powder is ultrafinely pulverized to 0.074 mm (through a 200 mesh sieve), dispersed in an aqueous solution at a weight ratio of corn yellow powder to water of 1:6, and placed in In the pressurized container, adjust the pressure to be 250kpa and process for 1h, then cool to room temperature; (2) add the corn yellow powder that is pressurized to the reactor and add it to the reactor, and control the protein mass concentration at 15%; (3) press The weight ratio of immobilized protease derived from fig (fig) to zein is 10:1. Add the enzyme at a temperature of 70°C, a shaking speed of 350rpm, and a constant pH under normal pressure. When the DH value of the reaction reaches 35 % when the reaction is terminated; (4) the reaction is terminated by filtering out the enzyme, and the remaining reaction solution is centrifuged at a high speed (15,000 rpm) for 15 min, and the sup...
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