Chemiluminescence detection kit based on corpuscle

Abstract

The invention relates to a chemiluminescent detection reagent kit based on microparticles. The chemiluminescent detection reagent kit comprises a vector coated with an antibody, an antigen or Protein A, a labelled antibody, a standard product of a determinant, a washing solution and a luminous solution, wherein the vector coated with the antibody, the antigen or the Protein A is a microparticle containing groups of -COOH, -NH2, -OH or -CHO; and the antibody, the antigen or the Protein A is coated on the surface of the microparticle in a chemical crosslinking mode. The labelled antibody is an antibody which is connected with acridinium ester or a derivant of the acridinium ester in the chemical crosslinking mode. The luminous solution comprises a hydrogen oxide and nitric acid aqueous solution and a sodium hydroxide and Triton X-100 aqueous solution. The microparticle can suspend on the sample solution, which is favorable for increasing full contact between the antibody, the antigen or the Protein A on the surface of the microparticle and the determinant and shortening the reaction time. The acridinium ester or the derivant of the acridinium ester is used as a label and can obtain a stable and high-strength signal.

Description

Technical field [0001] The invention relates to a chemiluminescence detection kit, especially a chemiluminescence detection kit based on microparticles, which belongs to the field of biomedical detection. Background technique [0002] Chemiluminescence detection kits use two kinds of detection substances (antibodies, antigens or other molecules that have affinity for specific test substances) to determine a certain substance in a sample. One of the detection substances is coated on a solid surface (hereinafter referred to as the solid phase), and the other detection substance is labeled with an enzyme or a chemiluminescent substance (hereinafter referred to as a label). During the reaction, the analyte and the label are bound to the solid phase. Washing to remove excess free markers, adding luminescent liquid, and converting chemical energy into light energy through reaction to emit light. By measuring the luminous intensity, the concentration of the analyte can be calculated. ...

AI Technical Summary

Problems solved by technology

The disadvantages of this method are: 1) when using an enzyme-labeled plate as a solid phase carrier, because the detection substance on the surface of the carrier cannot fully contact the analyte in the sample, the reaction efficiency is low and the reaction time is long, usually within 60 More than minutes, some even reach more than 3 hours

2) Since the biological activity of enzymes will change with environmental conditions (temperature, pH, etc.), the amount of light produced by the same amount of enzymes under different conditions is significantly different, and stable results cannot be obtained

3) The molecular weight of horseradish peroxidase is 40,000, and that of alkaline phosphatase is 68,000. Labeling may affect the activity of the test substance and cause steric hindrance

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