Insect disinfestation host mixed with green muscardine fungus native and Bacillus thuringiensis

A technology of Bacillus thuringiensis and Metarhizin, which is applied in the directions of biocides, biocides, animal repellents, etc., to achieve the effects of good insecticidal effect, environmental friendliness and strong promotion.

Inactive Publication Date: 2009-05-13
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to continuous, frequent, and high-dose use, the resistance of pests to commonly used chemical agents is increasing day by day. Plutella xylostella has been resistant to organophosphates, organochlorines, carba

Method used

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  • Insect disinfestation host mixed with green muscardine fungus native and Bacillus thuringiensis
  • Insect disinfestation host mixed with green muscardine fungus native and Bacillus thuringiensis
  • Insect disinfestation host mixed with green muscardine fungus native and Bacillus thuringiensis

Examples

Experimental program
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Effect test

Embodiment 1

[0015] Example 1 Indoor Toxicity Determination Experiment of Metarhizin, Bacillus thuringiensis (Bt) Single Agent and Mixture thereof to Plutella xylostella

[0016] Described metarhizine can be any one in metarhizine A, B or E, or their mixture of any ratio; Described Bacillus thuringiensis (Bt) refers to the bacterium that content is 8000IU / mg powder or equivalent.

[0017] Indoor virulence determination, the leaf drug film method was used. The metarhizine and Bacillus thuringiensis agents were prepared to the required concentrations, and 5 concentration gradients were set for each treatment, and 3 repetitions were set for each concentration. Clean and non-pesticide-polluted pakchoi leaves were dipped in the medicinal solution for 5 seconds, then taken out and dried in the shade, put into a petri dish with a diameter of 9 cm and covered with filter paper, and then inserted into 15 third-instar larvae of diamondback moth. Placed in a breeding room for feeding. The feeding ...

Embodiment 2

[0022] Embodiment 2: Indoor toxicity determination of metarhizine, Bt single agent and its mixture to Spodoptera litura

[0023] During the indoor toxicity test, appropriate amounts of medicaments were measured respectively, and acetone was used to make a mother liquor with a certain concentration and placed in the refrigerator (4° C.) for later use. During the test, all medicaments were diluted with 0.01% Tween 80 solution. Each treatment was replicated 3 times, and each replicate had 1 petri dish, and 15 third-instar Spodoptera litura larvae were placed in each petri dish. First, cut the leaves of cabbage into small pieces of 6-7 cm, soak them in the medicinal solution for about 5 seconds, take them out, dry them indoors, put the leaves into a petri dish connected with Spodoptera litura, and use wet cotton balls to moisturize. Change the cabbage leaves every 24 hours after the medicine and clean the petri dish. The experiment was repeated twice. Observe and record the surv...

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Abstract

The invention discloses a mixed disinsection main agent of bacillus thuringiensis and destruxins, wherein the mass ratio of destruxins to bacillus thuringiensis is 9-1:1-9. The destruxins and bacillus thuringiensis are mixed to determine the best mixing ratio, therefore the obvious synergistic action is obtained, the pesticide resistance problems of important pests of mustard family vegetables such as cabbage moth, cotton leafworm are effectively solved, with important value to control the cabbage moth and cotton leafworm and good and stable disinsection effect, strong practical application and popularization, friendly to the environment.

Description

technical field [0001] The invention belongs to the technical field of biopesticides, and in particular relates to a synergistic insecticidal main agent mixed with metarhizine and Bacillus thuringiensis and a preparation method thereof. Background technique [0002] Metarhizium anisopliae var. anisopliae, an entomopathogenic fungus, is secreted into the hemocoel of host insects during the infection process. Metarhizium anisopliae var. anisopliae is a cyclic peptide antibiotic. Insect effect, and has a new mechanism of action different from existing insecticides, mainly acting on the hemolymph immune system of insects, making them lose their immune defense capabilities. Metarhizin is easy to degrade, has no residue, and has low toxicity. It is of great economic significance to develop and utilize metarhizin. However, when metarhizine is used alone, the toxicity is not high enough, and the control effect is not ideal. At the same time, long-term use of the same insecticide i...

Claims

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Application Information

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IPC IPC(8): A01N63/00A01N63/04A01P7/00
Inventor 胡琼波任顺祥
Owner SOUTH CHINA AGRI UNIV
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