Degradation method for polychlorinated biphenyl with staphylococcus epidermidis

A technology of Staphylococcus epidermidis and polychlorinated biphenyls, which is applied in the field of pollution microorganism degradation, and can solve problems such as high price and secondary pollution.

Inactive Publication Date: 2009-05-13
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the methods to deal with PCBs pollution are mainly physical or ch...

Method used

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  • Degradation method for polychlorinated biphenyl with staphylococcus epidermidis
  • Degradation method for polychlorinated biphenyl with staphylococcus epidermidis
  • Degradation method for polychlorinated biphenyl with staphylococcus epidermidis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1 Isolation and Identification of PCB Degrading Bacteria

[0022] 1. Isolation of strains

[0023] (1) Collect activated sludge from Shanghai Songjiang Sewage Treatment Plant, place it in a 60cm long, 40cm wide, and 50cm high glass domestication device, and use an air compressor to aerate without adding nutrients and without water inflow and outflow exchange After 3 days, change the water, drain about 10L of mud-water mixture in the device, add nutrient solution and carry out uninterrupted aeration. After 10 days of cultivation, the waste liquid of PCBs was added to carry out acclimatization and cultivation while adding nutrient solution, and the acclimatization was cultivated for two months. Preparation of culture medium: 5 grams of glucose, 2 grams of urea, and 1 gram of potassium dihydrogen phosphate. Prepare a nutrient solution of about 10 liters.

[0024] (2) Precipitate the acclimatized water sample for 30 minutes, take the supernatant night, and then f...

Embodiment 2

[0031] Example 2 Degradation Analysis of Polychlorinated Biphenyls by Staphylococcus Epidermidis

[0032] Inoculate Staphylococcus epidermidis into sterilized acclimation medium (0.5g / L KH 2 PO 4 , 0.5g / L K 2 HPO 4 , 0.2g / L MgSO 4 , 0.1g / LCaCl 2 , 0.2g / LNaCl, 1.0g / L (NH 4 ) 2 SO 4 , 1g / L biphenyl), placed in a constant temperature shaker, 30°C, 150rpm, cultured for 14 days. Then inoculated in PCBs degradation medium (Degradation Medium) (0.5g / L KH 2 PO 4 , 0.5g / L K 2 HPO 4 , 0.2g / L MgSO 4 , 0.1g / L CaCl 2 , 0.2g / LNaCl, 1.0g / L(NH4) 2 SO 4 , 100mg / L 2,3',4', 5-tetrachlorobiphenyl-acetone solution, pH7.0), the sample was determined by high-performance liquid chromatography, and the degradation effect of the bacteria on PCBs was analyzed.

[0033] Liquid chromatography conditions:

[0034] ①Single-column single-detection system, 250mm long, 5mm inner diameter activated carbon column.

[0035] ②Carrier ratio: chromatography grade methanol 10%, purified water 90%

[...

Embodiment 3

[0042] Example 3 Effect of external carbon source on the degradation efficiency of polychlorinated biphenyls

[0043] (1) Insert Staphylococcus epidermidis into enrichment medium EM, place in a shaker at 30° C., 150 rpm, and enrich for 5 days.

[0044] (2) Three 50ml degradation medium DM were prepared, one of which was added with 2g / L glucose as an external carbon source, and placed in a 150ml Erlenmeyer flask. One bottle was left blank without inoculation, and the other two bottles were inoculated with 100ul enriched bacterial solution. Cultivate on a shaker at 30°C, 150rpm.

[0045] (3) Under aseptic operation conditions, take out 5ml of bacterial solution on 1d, 2d, 3d, 4d, and 5d after inoculation respectively.

[0046] (4) Sample pretreatment

[0047] ①Put the bacterial solution in a separatory funnel, and add dichloromethane three times. Add 2ml for the first and second time, and 1ml for the third time. Shake well after each addition and let stand for a while. Aft...

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Abstract

The invention relates to a method for degradation of polychlorinated biphenyls by staphylococcus epidermidis. The method comprises the following steps: inoculating staphylococcus epidermidis in a polychlorinated biphenyl degradation culture medium and subjecting the staphylococcus epidermidis to the shaking culture at 28-32 DEG C for 24-120 hours; and analyzing the degradation effect of the bacterial strain on the PCBs after the culture is subjected to high performance liquid chromatograph determination. By the addition of extra carbon source glucose, the method can ensure that the staphylococcus epidermidis is more efficient in degrading the polychlorinated biphenyls, thereby allowing the bacteria to obtain the conditions for degrading the polychlorinated biphenyl.

Description

technical field [0001] The invention belongs to the field of microbial degradation for pollution, and in particular relates to a method for degrading polychlorinated biphenyls by Staphylococcus epidermidis. Background technique [0002] Jensen first reported the pollution of polychlorinated biphenyls (PCBs) in the environment in 1966. Since then, the existence and persistence of PCBs have become a hot issue that people pay attention to. Due to the persistence and refractory nature of PCBs, PCBs are one of the most important pollutants in the environment, which are widely accumulated in soil, water, sediment and atmosphere. At present, the methods for dealing with PCBs pollution are mainly physical or chemical techniques, both of which are either expensive or cause secondary pollution. As an economical and effective method, microbial treatment technology has been adopted and researched by various countries. [0003] Microbes will secrete different enzymes to participate in ...

Claims

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Application Information

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IPC IPC(8): A62D3/02A62D101/22
Inventor 赵晓祥彭瑶任昱宗庞晓倩
Owner DONGHUA UNIV
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