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Antihypertensive active kyrine, preparation and uses thereof

An antihypertensive and active technology, applied in the field of antihypertensive active tripeptide and its preparation, can solve the problem that it is difficult to determine antihypertensive active peptide products, etc., and achieve good blood pressure lowering effect and good effect of inhibiting ACE activity.

Active Publication Date: 2009-05-13
BRIGHT DAIRY & FOOD CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Most of the so-called hypotensive peptide products reported in the literature are ACE inhibitors (ACEI), but they have not been verified by animal experiments, so it is difficult to determine that they are real antihypertensive active peptide products

Method used

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  • Antihypertensive active kyrine, preparation and uses thereof
  • Antihypertensive active kyrine, preparation and uses thereof
  • Antihypertensive active kyrine, preparation and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1 Preparation of Alcalase Enzyme Hydrolysis Active Peptide of Casein

[0017] Referring to CN 200710042207.5, the specific steps are:

[0018] 1. Enzyme reaction using enzyme reactor:

[0019] (1) Casein dissolution

[0020] Add 1000ml of water into the reactor, heat up to the optimum temperature of about 55°C, weigh 150g of casein (Shanghai Chemical Reagent Company), slowly and gradually put it into the water under magnetic stirring, adjust the pH to about 8.5 with 2mol / L NaOH, Under the condition of 55°C, all the casein was dissolved, so that the concentration of casein in the reactor was 15% (w / v, g / ml).

[0021] (2) Enzymolysis

[0022] According to the ratio of enzyme activity unit / substrate weight of 0.072AU / g casein, add 4.5g food-grade protease Alcalase (Novozymes Biotechnology Co., Ltd., 2.4L, enzyme activity 2.4AU / g), and maintain the reaction temperature at 55 ℃, and 2mol / L NaOH was added dropwise through the ZDJ-4A automatic potentiometric titrat...

Embodiment 2

[0029] Example 2 Preparation of antihypertensive active tripeptide of the present invention

[0030] 1. Size exclusion chromatography separation process

[0031] Sephadex G-15 was packed into a 1.6 x 100 cm column. Get the Alcalase enzymatic hydrolysis active peptide sample of casein of embodiment 1, dissolve with pH3.6, 0.02moL / L acetic acid buffer solution and be prepared into the solution 15mL of certain concentration 10mg / mL and put on the column, and with the same buffer solution at 42ml / h The flow rate is eluted, and at the same time, the wavelength (λ) is 220nm for detection with an ultraviolet detector, such as figure 2 As shown, a total of 6 peaks from peaks I to VI were separated, and the components of peak V were collected and freeze-dried.

[0032] 2. High performance liquid chromatography purification process

[0033] HPLC system: Waters600E (Waters company);

[0034] Analysis parameters:

[0035] Column: μBondapak C 18 3.9*300mm I.D. (Waters Company);

[...

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PUM

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Abstract

The invention discloses antihypertensive active tripeptide and a preparation method thereof. The amino acid sequence of the antihypertensive active tripeptide is Asn-Pro-Trp. The antihypertensive active tripeptide is obtained by hydrolyzing casein. The active tripeptide has good ACE inhibiting activity and antihypertensive function, and can be used for preparing antihypertensive drink, foods or medicine.

Description

technical field [0001] The invention relates to the field of bioactive peptides, in particular to an antihypertensive active tripeptide obtained by hydrolyzing casein and its preparation method and application. Background technique [0002] Animal milk, such as cow milk, is a biological fluid with complex components, the protein content of which is 32g / L, mainly composed of casein and whey protein, and casein accounts for about 80% (26g / L), which is containing A complex of several proteins of phosphorus, present in milk in colloidal form. Milk protein is a high-quality full-valent protein in nutrition, containing a variety of bioactive peptide fragments, which have many physiological functions. [0003] Most of the so-called hypotensive peptide products reported in the literature are ACE inhibitors (ACEI), but they have not been verified by animal experiments, so it is difficult to determine that they are real antihypertensive active peptide products. For example, in 1982,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K5/08C07K1/16A61K38/06A61P9/12
Inventor 宫霞郭本恒王荫榆
Owner BRIGHT DAIRY & FOOD CO LTD
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