Process for extracting anthocyanidin in blackcurrant by aqueous enzymatic method

A technology of anthocyanin and water enzymatic method, applied in the direction of fermentation, etc., can solve the problems of difficult operation, low juice yield, high viscosity, etc., and achieve the effect of low pollution, low cost and simple method

Inactive Publication Date: 2009-05-20
ZHEJIANG HUISONG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The problem with the above method is that there is a large amount of pectin in the blackcurrant fruit, and the berry is generally crushed to extract the pigment, but the pulp material system after mechanical crushing is very viscous, and it is difficult to directly extract the juice by pressing, because the juice The extra...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Using 2kg of blackcurrant fresh fruit as raw material, after crushing, add purified water with 20% of the weight of the pulp to the pulp, heat up to 40°C, then add pectinase with 0.03% of the weight of the pulp, stir evenly, keep warm for 5 hours, and the enzyme After solution, centrifuge to obtain the primary filtrate and peel residue. The fruit peels were repeatedly extracted 4 times with 0.1% citric acid aqueous solution, the dosage of the citric acid aqueous solution was 4 times the weight of the fruit peels, the extraction time was 1 hour each time, and the extraction temperature was 40°C. The extract was centrifuged to obtain the second filtrate. The primary filtrate and the secondary filtrate are mixed, then concentrated in vacuum at low temperature, the temperature is controlled at 55-60° C., and concentrated until the solid content is 2% concentrated liquid. Adopt D101 macroporous adsorption resin column, pass the column with citric acid aqueous solution with ...

Embodiment 2

[0026] Take 20kg of fresh black currant fruit, after crushing, put the pulp into the extraction bottle, add purified water with 60% of the weight of the pulp, heat up to 45°C, then add pectinase with 0.06% of the weight of the pulp, stir evenly, and keep warm After 3 hours, enzymatic hydrolysis and centrifugation to obtain the primary filtrate and peel residue. The fruit peel was repeatedly extracted three times with 0.2% citric acid aqueous solution, the dosage of the citric acid aqueous solution was 12 times the weight of the fruit peel, the extraction time was 2 hours, and the extraction temperature was 45°C. The extract was centrifuged to obtain the second filtrate. The primary filtrate and the secondary filtrate are mixed and then concentrated in vacuum at low temperature. The temperature is controlled at 55-60° C. and concentrated to a concentrated solution with a solid content of 1%. D101 macroporous adsorption resin column was used, and the column was acidified with c...

Embodiment 3

[0028]Take blackcurrant frozen fruit 100kg, after thawing and crushing, put the pulp into the extraction bottle, add purified water with 100% pulp weight, heat up to 50°C, then add pectinase with 0.1% pulp weight, and stir Evenly, keep warm for 1.5h, and centrifuge after enzymatic hydrolysis to obtain the primary filtrate and peel residue. The fruit peels were repeatedly extracted twice with 0.3% citric acid aqueous solution, the dosage of the citric acid aqueous solution was 20 times that of the fruit peels, the extraction time was 3 hours each time, and the extraction temperature was 50°C. The extract was centrifuged to obtain the second filtrate. The primary filtrate and the secondary filtrate are mixed, then concentrated in vacuum at low temperature, the temperature is controlled at 55-60° C., and concentrated to a concentrated solution with a solid content of 0.5%. Adopt D101 macroporous adsorption resin column, pass the column with citric acid aqueous solution with a pH...

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PUM

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Abstract

The invention discloses a process which uses an aqueous enzymatic method to extract anthocyanidin from blackcurrant. The fresh blackcurrant fruit is taken as raw material and then crushed, later purified water and pectinase are added into mashed fruit; by centrifugal separation after enzymatic hydrolysis, primary filtrate and peel residue are obtained; after the peel residue is extracted by acid solution and undergoes centrifugal separation, secondary filtrate is obtained; later the primary filtrate and the secondary filtrate are mixed and concentrated into concentration liquid under low temperature and vacuum state; and a resin column is attached on the concentration liquid, after elution with the acidic alcohol solution, low-temperature vacuum concentration and spray drying, a finished product of dry anthocyanidin powder is obtained. The invention effectively solves the problems of the existing extraction technology of the anthocyanidin of the blackcurrant that the process is complex, the energy consumption is large and the cost is high. The invention has the advantages of simple method, low cost, high anthocyanidin extraction rate, mild reaction condition, and low pollution degree to ecological environment, and is a new process for extracting the anthocyanidin from the blackcurrant, being worth of popularization.

Description

technical field [0001] The invention relates to a production process for extracting anthocyanins from blackcurrant, which belongs to the technical field of biochemical separation and purification. Background technique [0002] Black currant (Ribes nigrum L.), also known as black bean fruit, scientific name black currant (black currant), is the fruit of the saxifrage (saxifrage) plant of the genus Chalus. It is a small perennial shrub, mainly distributed in the northern hemisphere with relatively cold climate In cold regions, many countries are rich in black currants, and the production areas in our country are mainly distributed in the northeast and northwest regions. The fruit is black, nearly spherical, sweet and sour, fragrant, and rich in nutrition. After processing, it can be made into refreshing drinks, fruit wine, essence and other products. Black currant fruit is rich in phenolic compounds, mainly anthocyanins, in the prevention of cancer, improving vision, controll...

Claims

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Application Information

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IPC IPC(8): C12P17/06
Inventor 马全民聂复礼周昌平
Owner ZHEJIANG HUISONG PHARMA
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