Process for extracting anthocyanidin in blackcurrant by aqueous enzymatic method
A technology of anthocyanin and water enzymatic method, applied in the direction of fermentation, etc., can solve the problems of difficult operation, low juice yield, high viscosity, etc., and achieve the effect of low pollution, low cost and simple method
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Embodiment 1
[0024] Using 2kg of blackcurrant fresh fruit as raw material, after crushing, add purified water with 20% of the weight of the pulp to the pulp, heat up to 40°C, then add pectinase with 0.03% of the weight of the pulp, stir evenly, keep warm for 5 hours, and the enzyme After solution, centrifuge to obtain the primary filtrate and peel residue. The fruit peels were repeatedly extracted 4 times with 0.1% citric acid aqueous solution, the dosage of the citric acid aqueous solution was 4 times the weight of the fruit peels, the extraction time was 1 hour each time, and the extraction temperature was 40°C. The extract was centrifuged to obtain the second filtrate. The primary filtrate and the secondary filtrate are mixed, then concentrated in vacuum at low temperature, the temperature is controlled at 55-60° C., and concentrated until the solid content is 2% concentrated liquid. Adopt D101 macroporous adsorption resin column, pass the column with citric acid aqueous solution with ...
Embodiment 2
[0026] Take 20kg of fresh black currant fruit, after crushing, put the pulp into the extraction bottle, add purified water with 60% of the weight of the pulp, heat up to 45°C, then add pectinase with 0.06% of the weight of the pulp, stir evenly, and keep warm After 3 hours, enzymatic hydrolysis and centrifugation to obtain the primary filtrate and peel residue. The fruit peel was repeatedly extracted three times with 0.2% citric acid aqueous solution, the dosage of the citric acid aqueous solution was 12 times the weight of the fruit peel, the extraction time was 2 hours, and the extraction temperature was 45°C. The extract was centrifuged to obtain the second filtrate. The primary filtrate and the secondary filtrate are mixed and then concentrated in vacuum at low temperature. The temperature is controlled at 55-60° C. and concentrated to a concentrated solution with a solid content of 1%. D101 macroporous adsorption resin column was used, and the column was acidified with c...
Embodiment 3
[0028]Take blackcurrant frozen fruit 100kg, after thawing and crushing, put the pulp into the extraction bottle, add purified water with 100% pulp weight, heat up to 50°C, then add pectinase with 0.1% pulp weight, and stir Evenly, keep warm for 1.5h, and centrifuge after enzymatic hydrolysis to obtain the primary filtrate and peel residue. The fruit peels were repeatedly extracted twice with 0.3% citric acid aqueous solution, the dosage of the citric acid aqueous solution was 20 times that of the fruit peels, the extraction time was 3 hours each time, and the extraction temperature was 50°C. The extract was centrifuged to obtain the second filtrate. The primary filtrate and the secondary filtrate are mixed, then concentrated in vacuum at low temperature, the temperature is controlled at 55-60° C., and concentrated to a concentrated solution with a solid content of 0.5%. Adopt D101 macroporous adsorption resin column, pass the column with citric acid aqueous solution with a pH...
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