Method for preparing antibacterial metabolin of Propionibacterium and use thereof

A technology of Propionibacterium tereki and bacteriocin is applied in the field of preparation and application of Propionibacterium tereki bacteriocin antibacterial metabolites, and achieves the effects of low production cost, wide antibacterial spectrum and convenient operation.

Inactive Publication Date: 2009-06-03
SHANGHAI INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The preparation method and application of antibacterial metabolites of Propionibacterium tertii bacteriocin have not yet been reported

Method used

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  • Method for preparing antibacterial metabolin of Propionibacterium and use thereof
  • Method for preparing antibacterial metabolin of Propionibacterium and use thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0027] Seed medium: tryptone 10g, yeast extract 10g, 60% sodium lactate 16.7ml, K 2 HPO 4 0.25g, MnSO 4 0.005g, add distilled water to 1000ml, pH7.0

[0028] Fermentation medium: tryptone 10g, yeast extract 10g, glucose 20g, K 2 HPO 4 0.25g, MnSO 4 0.005g, 20g agar, add distilled water to 1000ml, pH7.0

[0029] Inoculate Propionibacterium terreii with the preservation number ATCC4874 in the seed medium and culture it at 30°C for 48 hours to obtain the seed liquid, then insert the seed liquid into the cooled fermentation medium according to the inoculum amount of 4% of the fermentation volume, and culture at 30°C for 5 days Finally, the fermentation broth of antibacterial metabolites containing bacteriocins is obtained. The fermented broth obtained is subjected to rotary evaporation to reduce the volume to 1 / 5 of its original size, and then freeze-dried on a freeze dryer until the water is completely sublimated, and the dry powder of the fermented broth is obtained and th...

Embodiment 2

[0039] Seed medium: tryptone 10g, yeast powder 10g, 60% sodium lactate 16.7ml, K 2 HPO 4 0.25g, MnSO 4 0.005g, add distilled water to 1000ml, pH7.0

[0040] Fermentation medium: tryptone 10g, yeast extract 10g, lactose 20g, K 2 HPO 4 0.25g, MnSO 4 0.005g, 20g agar, add distilled water to 1000ml, pH7.0

[0041] Inoculate Propionibacterium terreii with the preservation number ATCC 4874 in the seed medium and culture it at 30°C for 48 hours to obtain the seed liquid, insert the seed liquid into the cooled fermentation medium according to the inoculation amount of 4% of the fermentation volume, and culture at 30°C After 5 days, antibacterial metabolites containing bacteriocins were obtained. The fermented broth obtained is subjected to rotary evaporation to reduce the volume to 1 / 5 of its original size, and then freeze-dried on a freeze dryer until the water is completely sublimated, and the dry powder of the fermented broth is obtained and then sealed and stored at 4°C.

Embodiment 3

[0043] Seed medium: tryptone 10g, yeast powder 10g, 60% sodium lactate 16.7ml, K 2 HPO 4 0.25g, MnSO 4 0.005g, add distilled water to 1000ml, pH7.0

[0044] Fermentation medium: corn steep liquor 10g, yeast extract 10g, sucrose 30g, K 2 HPO 4 0.25g, MnSO 4 0.005g, 20g agar, add distilled water to 1000ml, pH7.0

[0045] Inoculate Propionibacterium terreii with the preservation number ATCC 4874 in the seed medium and culture it at 30°C for 48 hours to obtain the seed liquid, insert the seed liquid into the cooled fermentation medium according to the inoculation amount of 5% of the fermentation volume, and culture at 30°C After 6 days, antibacterial metabolites containing bacteriocin were obtained. The fermented broth obtained is subjected to rotary evaporation to reduce the volume to 1 / 6 of its original size, and then freeze-dried on a freeze dryer until the water is completely sublimated, and the dry powder of the fermented broth is obtained and then sealed and stored at ...

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Abstract

The invention discloses a method for preparing antibacterial metabolin of Propionibacterium, the Propionibacterium (Propionibacterium.thoenii) is stored in American Type Culture Collection (ATCC) with the storing number of ATCC 4874. In the invention, the Propionibacterium is activated and fermented by microorganism fermentation technology to obtain the antibacterial metabolin containing the Propionibacterium. The antibacterial metabolin of Propionibacterium can be used in food anticorrosion and fresh-keeping, disinfector, anti-infection medicine, animal and plant disease control and the medicine field for inhibiting gram-negative bacteria, mould and microzyme. The method for preparing the antibacterial metabolin is convenient in operation, is low in production cost and has wide application prospect.

Description

technical field [0001] The present invention relates to a preparation method of natural antibacterial metabolites containing bacteriocins produced by Propionibacterium terzelii strains, and the antibacterial metabolites of the above-mentioned Propionibacterium terzelii bacteriocins are used in food preservation, disinfectants, anti-infective drugs, animals and plants Uses in disease control and medicine. Background technique [0002] The most common food preservatives are chemical preservatives such as benzoic acid, sodium benzoate, sorbic acid, and potassium sorbate. However, the potential safety hazards of chemical preservatives cannot be ignored more and more, while biological preservatives have the advantages of many antibacterial substances, wide sources, safety, non-toxicity, and stable performance. As a new type of natural preservatives, they have huge applications in food preservation. potential has drawn increasing attention. [0003] Bacteriocins are a large clas...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/00A23L3/3526A01N63/02A01P3/00A61L2/16A61K38/17A61P31/00C12R1/01
Inventor 荣绍丰马悦培高红亮常忠义贾彩凤陈玉梅冯晗
Owner SHANGHAI INST OF TECH
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