Compositions and methods to add value to plant porducts, increasing the commercial quality, resistance to external factors and polyphenol content thereof
A composition, compound technology, applied in the fields of botanical equipment and methods, plant preservation, horticultural methods, etc.
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Embodiment 1
[0167] Embodiment 1: the preparation of the ferulic acid preparation of dilution
[0168] Formulations containing only ferulic acid in the appropriate working concentration range were prepared as previously described. For this purpose, 3 diluted formulations (referred to as "Formulation A") each with a final volume of 0.5 liters were prepared. The first formulation - Formulation A-I contained ferulic acid at a final concentration of 10 mM, the second formulation - Formulation A-II contained ferulic acid at a final concentration of 20 mM, and the last formulation - Formulation A-III contained ferulic acid at a final concentration of 40 mM acid. The composition of the final diluted formulation is shown in the table below.
[0169] Table 4: Composition of Diluted Formulation A
[0170]
[0171] Formulations A-I, A-II and A-III were prepared as follows. First, appropriate equal amounts of water and absolute ethanol (Merck S.A., Santiago, Chile) were mixed to form a 20% et...
Embodiment 2
[0172] Example 2: Ferulic acid, p-coumaric acid and caffeic acid and the mixing of ferulic acid and p-coumaric acid Preparation of concentrated preparations of substances and mixtures of caffeic acid and p-coumaric acid
[0173] Subsequently, a new set of formulations (referred to as "Formulation B") was prepared comprising a mixture of certain active compounds of the invention. The formulations also contain different concentrations of the active compounds, either alone or as part of a mixture. As a comparative reference against the previous group of formulations (Formulation A), a formulation containing only two different concentrations of ferulic acid was used. Formulation B is initially prepared as a concentrated solution of a compound of the invention in a suitable solvent. In formulation B, dimethylformamide (DMF) is used as solvent in a slight excess relative to that required to dissolve the required amount of active compound without heating. In this way, by suitab...
Embodiment 3
[0179] Example 3: Preparation of ferulic acid, p-coumaric acid and caffeic acid from concentrated solutions And the mixture of ferulic acid and p-coumaric acid and the mixture of caffeic acid and p-coumaric acid Diluents and dilute preparations
[0180] Concentrated Formulation B of Table 5 was diluted in dilutions prepared from the components and ratios shown in Table 6 immediately before administration. Diluents contain humectant-adherents and emulsifiers (Zoom , from ANASAC S.A.C.I., Santiago, Chile) and a volatile pH buffer (ammonium bicarbonate), both dissolved in water.
[0181] Table 6: Recipe for Diluent
[0182]
[0183] The dilution was prepared by dissolving 3.45 g of ammonium bicarbonate in 450 ml of water with stirring. Once the solids are completely dissolved, add 0.25ml of Zoom , and homogenize the solution for another 1 min. Finally, enough water is added to make 500ml.
[0184] Dilution solutions were prepared using this dilution to provide the...
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