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Rumex nepalensis Spreng. extract as well as preparation method and use thereof

A technology of Nepal sorrel extract, applied in the application of Nepal sorrel extract in the preparation of α-glucosidase inhibitor drugs, the extraction of Nepal sorrel extract, the application field of preparation of hypoglycemic drugs, can solve the problem of High cost, few manufacturers, intestinal side effects and other problems, to achieve the effect of low price, wide distribution and abundant resources

Inactive Publication Date: 2011-05-04
HENAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing drugs of this type are costly, have few manufacturers, and are accompanied by intestinal side effects

Method used

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  • Rumex nepalensis Spreng. extract as well as preparation method and use thereof
  • Rumex nepalensis Spreng. extract as well as preparation method and use thereof
  • Rumex nepalensis Spreng. extract as well as preparation method and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The extract of sorrel sorrel in this example is prepared by the following method: take dried root of sorrel sorrel, pulverize it, soak it in petroleum ether for 12 hours, and extract it in a Soxhlet extractor under continuous reflux for 3 times, each time for 1 hour , to obtain the petroleum ether extract (RNRP) of Nepalese sorrel root; after evaporating the petroleum ether solvent, soak in ethyl acetate for 12 hours, and then continuously reflux extraction in a Soxhlet extractor for 3 times, each time for 1 hour, to obtain Nepalese sorrel root Ethyl acetate extract (RNRE); after evaporating the ethyl acetate solvent, immerse in methanol for 12h, and then continuously reflux extraction in a Soxhlet extractor for 3 times, each time for 1h, to obtain Nepal sorrel root methanol extract (RNRM ).

Embodiment 2

[0023] The Nepal sorrel extract of the present embodiment is prepared by the following method: take the dried above-ground part of Nepal sorrel, pulverize it, soak it in petroleum ether for 12 hours, and then continuously reflux extract it in a Soxhlet extractor for 3 times, each time for 1 hour, Obtain the petroleum ether extract (RNAP) of the aerial part of Rumex Nepal; after evaporating the solvent, soak it in ethyl acetate for 12 hours, and extract it by continuous reflux in a Soxhlet extractor for 3 times, each time for 1 hour, to obtain the ethyl acetate of the aerial part of Rumex Nepal Ester extract (RNAE); after evaporating the solvent, soak in methanol for 12 hours, and then continuously reflux extraction in a Soxhlet extractor for 3 times, each time for 1 hour, to obtain the methanol extract of the aerial part of Nepal sorrel (RNAM).

[0024] The yields of different solvent extracts of Nepal sorrel are shown in Table 1.

Embodiment 3

[0026] This example is the test for inhibiting α-glucosidase activity of Nepal sorrel extract

[0027] Method: Microplate method

[0028] Principle: α-D-glucosidase catalyzes the hydrolysis of 4-nitrobenzene-α-D-glucopyranoside (PNP-G) to produce nitrophenol (PNP, yellow substance, with maximum absorption around 400m), α -Glycosidase inhibitors can inhibit the binding of α-glucosidase to the substrate to reduce the release of PNP. The enzyme inhibitory activity of the extract was calculated by the change of PNP content in the reaction system within a certain period of time.

[0029] Instruments: MultiskanMK3 microplate reader (ThermoElectron); LRH-150 constant temperature incubator (Shanghai Yiheng Technology Co., Ltd.); DELTA320 PH meter (Mettler-Toledo); electronic balance (Mettler-Toledo); rotary evaporator (Heidolph).

[0030]Reagent: α-glucosidase (Sigma Company, EC3.2.1.20, frombaker'syeast, batch number: 105K1313), p-nitrophenyl-α-D-glucopyranoside (PNPG, Sigma Compan...

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Abstract

The invention discloses a Rumex nepalensis Spreng. extract and an extraction method and application thereof. The extract comprises Rumex nepalensis Spreng. roots and a petroleum ether extract, an ethyl acetate extract or a methanol extract of overground parts of Rumex nepalensis Spreng.. As proved by experimental results, various extracts of the Rumex nepalensis Spreng. all have obvious function of inhibiting alpha-glucuronide, and have dose-effect relationship under certain concentration, wherein the suppression ratio of the various extracts all reaches 100 percent under the same concentration (1.5 mg / ml), and is more than that of a positive control drug (68.43 percent); and the value of the half inhibition concentration IC50 of the various extracts is all less than 100 [mu]g / ml, and is far less than that of the positive control drug (1,081.27 [mu]g / ml).

Description

technical field [0001] The present invention relates to a kind of Nepalese sorrel extract, also relates to a kind of extraction method of this Nepalese sorrel extract, and its application in the preparation aspect of hypoglycemic medicine; application of pharmaceuticals. Background technique [0002] Nepal sorrel (Rumexnepalensis Spreng.) is a plant of the genus Rumex in the family Polygonaceae, distributed in Henan, Shaanxi, Gansu, Qinghai, Hubei, Sichuan, Yunnan and Tibet provinces. The whole herb is used as medicine, which has the effect of clearing heat and detoxifying, cooling blood and stopping bleeding. It is a common folk herbal medicine. Modern research shows that Nepal sorrel root contains active protein-lectin, which can cause agglutination reaction of 3% rabbit red blood cells. In addition, it has been reported that Nepal sorrel root methanol extract has laxative and sedative effects. However, there is no research report on the chemical constituents and other ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/70A61P3/10A61K125/00
Inventor 康文艺岳淑梅陈百泉刘瑜新宋艳丽
Owner HENAN UNIVERSITY
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