Protein imprinted material and use thereof for removing albumin from human blood serum
A human serum and molecular imprinting technology, applied to the application field of albumin, can solve the problems of high preparation cost, sample contamination, dye ligand shedding, etc., and achieve the effects of simple operation process, low production cost, and reduced loss.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment Construction
[0022] A western blot material, which can be prepared as follows:
[0023] (1) Mix functional monomers, cross-linking agents, porogens, surfactants and template proteins in a phosphate buffer with a pH of 5-6;
[0024] (2) After the above-mentioned mixture is mixed uniformly, nitrogen gas is passed into it, and then an initiator and a speed-accelerator are respectively added therein to carry out a polymerization reaction, the polymerization temperature is controlled between 20-30° C., and the reaction time is 5-24 hours;
[0025] The amount of each substance used in the process of synthesizing imprinted materials is shown in the table below.
[0026]
[0027]
[0028] (3) After the imprinting material was synthesized according to the amount in the above table, the template protein on the polymer was eluted with a volume concentration of 9-11% acetic acid mixture containing 9-11% sodium dodecylsulfonate by weight. Subsequently, the polymer is washed with water and pH 5-6...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com