Difructose anhydride-containing composition and use thereof
A kind of double fructose anhydride, containing technology, applied in the field of DFA-containing composition, can solve the problem of not clearly pointed out or mentioned, producing organic acid and the like
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Embodiment 1
[0099] The present invention uses the Ussing chamber to confirm whether DFAIII can promote the absorption of magnesium as follows.
[0100] The test objects used in this test are Sprague-Dawley male mice (average weight about 260 g) aged about 7-8 weeks. The mice were about 6 weeks old when purchased (Japan SLC Co., Ltd.), and were fed with commercially available solid feed (CE-2, Japan CLEA Co., Ltd.) until the day before the experiment.
[0101] On the day of the experiment, sleeping pills were fed into the abdominal cavity of the mice, and the small intestines (jejunum and ileum) and large intestines (cecum and colon) were taken out under anesthesia. After spreading out the sundries in their intestines, peel off the serosa and smooth tendon layer, and make a specimen Ussing chamber composed of mucosa and mucosal tissue (the actual effective area is 0.67Cm 2 ).
[0102] On the other hand, the mucosal buffer and serosal buffer are prepared respectively. The basic compositi...
Embodiment 2
[0123] MgCl in lateral membrane buffer 2 ·6H 2 O 1mM replaced by ZnSO 4 ·7H 2 O 0.0125mM, when measuring the absorption rate of zinc, the concentration of ions is set to 0.0125mM, and the serous membrane buffer is the same, except that ZnSO is used instead 4 ·7H 2 Except 0 1mM, all the other are the same as embodiment 1 to measure the speed of zinc absorption.
[0124] The results obtained are shown in Figure 2. It can be clearly shown that as the concentration of DFAIII increases, the absorption of zinc also increases.
Embodiment 3
[0126] MgCl in lateral membrane buffer 2 ·6H 2 O 1mM was replaced by CuCl 2 2H 2 O 0.0125mM, when measuring the absorption rate of copper, the concentration of ions is set to 0.0125mM, and the serous membrane buffer is the same, except that CuCl is used instead 2 2H 2 Except 0 1mM, all the other are the same as embodiment 1 to measure the speed of copper absorption.
[0127] The results obtained are shown in Figure 3. It can be clearly shown that as the concentration of DFAIII increases, the uptake of copper also increases.
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