Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation of embedding immobilized effective microorganism gel bead

A microorganism and gel technology, applied in the field of microbial cell immobilization, can solve the problems of easy water absorption and swelling in service life, slow hardening, poor biological denitrification effect of wastewater, etc. effect of life

Inactive Publication Date: 2009-07-08
XIAMEN UNIV
View PDF0 Cites 41 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0017] The purpose of the present invention is to overcome the difficulties in forming gel beads and slow hardening during the embedding process, as well as the defects of gel beads strength, service life, and easy water absorption and swelling after embedding, and the biological denitrification effect of wastewater is relatively low. To overcome the poor problem, provide an embedding immobilized effective microbial gel that can not only effectively solve the shortcomings of the preparation and use of gel beads, but also use effective microorganisms (EM) as embedded bacteria, which can effectively improve the effect of biological nitrogen removal. The preparation method of glue pellet

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation of embedding immobilized effective microorganism gel bead
  • Preparation of embedding immobilized effective microorganism gel bead
  • Preparation of embedding immobilized effective microorganism gel bead

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0029] 1. Preparation of EM bacterial solution

[0030] Preparation of EM rejuvenation solution: prepare EM expansion solution according to the volume ratio, EM-1:molasses:water=3:3:94. Sealed storage at room temperature, after the pH value drops below 3.5 and has a sweet and sour smell, the EM rejuvenation solution can be obtained.

[0031] Preparation of EM centrifugal liquid: Centrifuge the EM rejuvenation liquid prepared in the previous step at a speed of 5000 r / min for 20 min, discard the supernatant, and collect the concentrated EM cells enriched at the bottom of the centrifuge tube. Every 10mL of EM rejuvenation liquid is centrifuged to obtain EM concentrated bacteria containing the amount of bacteria equivalent to 10mL of EM rejuvenation liquid.

[0032] 2. Preparation of Shell Powder

[0033] Pulverize the oyster shells with a traditional Chinese medicine grinder and pass through an 80-mesh sieve to obtain shell powder.

[0034] 3. Preparation of Immobilized Pellet...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

A preparation method of embedding immobilized effective microorganism gel pellets relates to a microbial cell immobilization method for saprobe denitrification, specifically to a method taking polyvinyl alcohol and sodium alginate as the embedding agent, calcium chloride and boric acid as a cross linker and shell powder as additives to embed the effective microorganism. The invention provides a preparation method of embedding immobilized effective microorganism gel pellets which can not only effectively resolve the defects in the preparation and use process of the gel pellet, but also can effectively improve the biological denitrification effect by taking the effective microorganism as embedding thalli. The method includes: mixing uniformly the embedded agents and additives, dissolving in water, completely dissolving and mixing uniformly and then cooling to below 40 DEG C, mixing with the centrifuged effective microorganism concentrated bacterium liquor, stirring, and then adding into the cross-linker to form the gel pellets.

Description

technical field [0001] The invention relates to a microbial cell immobilization method for biological denitrification of sewage, in particular to a method using polyvinyl alcohol and sodium alginate as embedding agents, calcium chloride and boric acid as crosslinking agents, and shell powder as embedding agents. Additives, the method of embedding effective microorganisms (Effective Microorganisms, referred to as EM). Background technique [0002] Microbe embedding and immobilization technology is a relatively new technology in the field of wastewater biological treatment. It encloses microorganisms in natural polymer polysaccharides or synthetic polymer gels, thereby immobilizing microorganisms. Compared with the ordinary activated sludge method, the embedding and immobilization technology has the following advantages: preventing microbial loss, high microbial concentration in the reactor, anti-toxin and impact load, good settling performance, and conducive to solid-liquid s...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N11/04C12R1/00C02F3/34
Inventor 熊小京李博郑天凌赵志新
Owner XIAMEN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com