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Rapid measuring method for high-salt trade waste BOD

A technology for industrial wastewater and measurement methods, applied in the direction of testing water, material inspection products, etc., can solve the problems of easy cracking of sol-gel films, restricted use, easy swelling of graft copolymers, etc., and achieve simple and fast measurement methods, High accuracy and good stability

Inactive Publication Date: 2009-07-08
HUAIYIN INSTITUTE OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The five-day working method is time-consuming and cumbersome, and requires relatively high skills for operators; for emergency testing, data cannot be provided in time; in addition, industrial wastewater, especially high-salt industrial wastewater, is very unfavorable to the growth and reproduction of microorganisms, so high-salt industrial wastewater BOD detection of industrial wastewater has always been a difficult problem
However, the disadvantages of sol-gel film cracking and graft copolymer swelling limit the use of such materials.

Method used

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  • Rapid measuring method for high-salt trade waste BOD
  • Rapid measuring method for high-salt trade waste BOD
  • Rapid measuring method for high-salt trade waste BOD

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0022] Example 1: Linear response range and response time

[0023] The GGA standard solution commonly used in BOD electrode performance testing is made of small molecular substances: glucose and glutamic acid, so the microorganisms absorb quickly and the electrode sensitivity is high; As a result, the response sensitivity of the electrode to the wastewater in the factory area is not high, so the working curve obtained with the standard BOD solution is not universal. It is necessary to choose a typical high-salt wastewater in the factory area as the standard in order to obtain a high-accuracy test that can be used for the detection of wastewater in the factory area. Working curve.

[0024] Chondroitin sulfate is a pharmaceutical raw material extracted from animal cartilage, laryngeal bone, nasal bone, trachea and bone tendon. Proteolysis, salting out and other methods are commonly used in its production. The production wastewater is a typical high-salt and high-BOD Industrial ...

example 2

[0028] Example 2: Effect of Salt Concentration

[0029] Generally speaking, the salt concentration of high-salt industrial wastewater is in the range of 3% to 10%, so this experiment chooses different salt concentrations for long-term (>12h) online investigation; image 3 0%, 5%, 10% and 15% salt concentration, the time-current curves of the BOD electrode detecting wastewater samples with the same BOD concentration.

[0030] Depend on image 3 It can be seen that the response time of the electrode is longer under the condition of no salt, and the electrode can only work stably for about 3 hours, and then the current gradually decreases and tends to zero; under the condition of 5% and 10% salt concentration, the electrode responds quickly, and The performance is stable; and under the condition of 15% salt concentration, the response time of the electrode is the longest, and the stability of the electrode is poor.

[0031] The activity and stability of most enzymes in halo-tol...

example 3

[0032] Example 3: Electrode Stability

[0033] Insert the electrode into 20ml of blank phosphate buffer solution, turn on the magnetic stirrer, after the current is stable, add 200μL of wastewater sample, and record the response current; take out the electrode, insert it into the blank phosphate buffer solution, and after standing for 5 minutes, the output current will slow down. Slowly return to the initial value of the first measurement, then add 200 μL of wastewater sample, record the response current, and repeat the above operation; plot the relationship between the response current and the number of times the electrode detects the wastewater with the same BOD value, as shown in Figure 4 shown.

[0034] Depend on Figure 4 It can be seen that the response current of the electrode can reach more than 90% of the first measured value in 18 consecutive tests, and the main reason for the decrease in the response value of the electrode during repeated use may be that it repeat...

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Abstract

The invention discloses a quick measuring method of BOD in hyperhaline industrial waste water. The method comprises the following steps: taking 10g sludge from a hyperhaline waste water sewage outfall; adding 90mL sterilized normal saline into the sludge; carrying out streaking on a beef extract peptone culture medium plate for 2d at a temperature of 37 DEG C till obtaining pure salt-tolerant bacterial strain; inoculating the bacterial strain in beef extract peptone culture medium to carry out shaking culture inside a constant temperature shaking box for 24h, centrifugation for 5min at a rotating speed of 4,500r / min and washing by means of 0.9 percent NaCl solution, thereby obtaining salt-tolerant bacteria; dispersing 5mg carbon nano tube in water to form 5mg.mL carbon nano tube suspension; preparing the salt-tolerant bacteria into 0.2 g.mL bacterial suspension by phosphate buffer solution; mixing the bacterial suspension with the carbon nano tube suspension according to the volume ratio of 1:2, and evenly coating 30 mu L mixed suspension on cellulose film with the aperture equal to 0.2 mu; cooling the cellulose film semidry and fixing the film on a dissolved oxygen electrode to form a BOD electrode; and setting a dissolved oxygen analyzer as automatic measuring and recording output current in time by magnetic data acquisition software. The quick measuring method has the advantages of simplicity and quickness, high accuracy and ideal stability.

Description

technical field [0001] The invention relates to a method for measuring BOD, in particular to a method for rapidly measuring BOD in high-salt industrial wastewater. Background technique [0002] Biochemical oxygen demand (BOD) refers to the amount of dissolved oxygen consumed by aerobic microorganisms during the biochemical oxidation process of oxidative decomposition of organic matter per unit volume of water under the condition of dissolved oxygen. BOD detection often uses the five-day work method (BOD 5 ) or the seven-day work method (BOD 7 ), the five-day working method is the oxygen consumption value obtained by taking 5 days as the standard time for measuring BOD under the condition of 20°C, sufficient oxygen, and no agitation. The five-day working method is time-consuming and cumbersome, and requires relatively high skills for operators; for emergency testing, data cannot be provided in time; in addition, industrial wastewater, especially high-salt industrial wastewa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/18
Inventor 贾建波陈静
Owner HUAIYIN INSTITUTE OF TECHNOLOGY
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