Beta-mannanase for feeding and preparation thereof

A technology of mannanase and feeding, which is applied in the field of microbial fermentation and enzyme engineering, can solve the problems of low thermal stability and pH stability, and achieve the effect of low production cost and reduced production cost

Inactive Publication Date: 2009-07-15
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES +1
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AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to screen a kind of fermentation raw material that can use abundant and cheap agricultural and sideline products for the β-mannanase produced by the above-mentioned existing Aspergillus niger strains, which has low thermal stability and pH stability. The produced β-mannanase is a new strain of Aspergillus niger with strong heat and pH stability; another purpose of the present invention is to propose a method for producing β-mannanase by fermentation of the new strain

Method used

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  • Beta-mannanase for feeding and preparation thereof
  • Beta-mannanase for feeding and preparation thereof
  • Beta-mannanase for feeding and preparation thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1: (beta-mannanase production and preparation 1)

[0039] In this example, β-mannanase was prepared according to the following steps:

[0040] (1) Preparation of media of various levels of Aspergillus niger strain MA-56: including,

[0041] ① Inclined seed medium: peeled potato 200g / l, sucrose 20g / l, agar 20g / l, natural pH, sterilized by 0.1Mpa for 20min;

[0042] ②Solid seed culture medium: put 10g of culture medium prepared by bran, soybean meal and konjac powder in a ratio of 80:19:1 in weight into a 300ml triangular flask, add tap water in a ratio of 1:1 to the weight of solid matter, and stir Evenly, sterilized by 0.1Mpa for 30min;

[0043] ③Solid production medium: First, prepare a solid medium by bran, soybean meal and konjac flour in a ratio of 75:23:3 by weight; then add water to the solid medium and water in a ratio of 1:1.3 by weight and stir evenly In a cloth bag, sterilize at 0.1Mpa for 30min, after cooling, put it evenly in a curved plate, an...

Embodiment 2

[0048] Embodiment 2: (beta-mannanase production preparation 2)

[0049] In this example: the preparation of step (1) aspergillus niger strain MA-56 culture medium at all levels: 3. solid production medium: first bran, soybean meal and konjac flour are formulated into solid medium by weight 88:13:1 ratio ; Then add water to the solid medium and water according to the ratio of 1:1.1 by weight, stir evenly, put it into a cloth bag, sterilize it at 0.1Mpa for 30min, and place it evenly in a curved plate after cooling, the thickness of the fermented material is 2-3cm;

[0050] Step (4) xylanase fermentation production: mix the solid seed spore suspension in step (3) with the solid production medium in step (1)③ in a ratio of 15:100 in parts by weight, and put it in a koji room at 28°C for fermentation and culture 56h to obtain β-mannanase enzyme koji for feeding;

[0051] Step (5): through the detection of β-mannanase activity for feed, it reaches 1×10 5 ~1.3×10 5 U / g;

[0052]...

Embodiment 3

[0053] Embodiment 3: (beta-mannanase production preparation 3)

[0054] In this example: step (1) preparation of various levels of medium for Aspergillus niger strain MA-56: ③ solid production medium: first prepare solid medium by bran, soybean meal and konjac flour in a ratio of 62:37:6 by weight ; Then add water to the solid medium and water according to the weight ratio of 1:0.9, stir evenly, put it into a cloth bag, sterilize it at 0.1Mpa for 30min, and place it evenly in a curved plate after cooling, the thickness of the fermented material is 2-3cm;

[0055] Step (4) xylanase fermentation production: mix the solid seed spore suspension in step (3) with the solid production medium in step (1)③ in a ratio of 30:100 in parts by weight, and place it in a koji room at 28°C for fermentation and culture 56h to obtain β-mannanase enzyme koji for feeding;

[0056] Step (5): through the detection of β-mannanase activity for feed, it reaches 1×10 5 ~1.3×10 5 U / g;

[0057] All th...

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Abstract

The invention discloses a [beta]-Mannanase in feed and a preparation method thereof, belonging to the technical fields of microbial fermentation and enzyme engineering. The invention obtains, by means of mutagenesis and sieving, an Aspergillus niger MA-56 CGMCC No.2722 which can take an abundant and inexpensive agricultural byproduct as a fermentation raw material and in which the resulting acidic [beta]-Mannanase has relatively strong stability to heat and pH; and the invention proposes a production method comprising the steps of: taking the strain as a production strain to be inoculated in a solid culture medium that is formulated by bran, dregs of beans and konjaku flour based on the weight part of 60-90:10-40:1-6; fermenting, culturing, drying and detecting the strain to prepare the [beta]-Mannanase in feed. The enzyme keeps the temperature for 1 hour at 70 DEG C, which still can maintain the enzyme activity of about 85%, and keeps the temperature for 2 hours in a pH range from 3.0 to 9.0, which still can maintain the enzyme activity of over 90%, therefore, the enzyme is relatively suitable for being used as a feed additive. The [beta]-Mannanase in feed can be popularized and applied in the field of feed production.

Description

technical field [0001] The invention relates to the technical field of microbial fermentation and enzyme engineering, in particular to a feed beta-mannanase fermented by a new strain of Aspergillus niger and a preparation method thereof. Background technique [0002] The main rations for pigs and chickens in my country are corn / soybean meal rations. Although monogastric animals have a high digestibility of corn, the energy utilization rate of soybean meal is only 50% to 60%. The main reason is that soybean meal contains 1.3 % of β-mannan, it has a strong anti-nutritional effect on animals, including humans, affecting energy metabolism and protein synthesis. β-mannanase can degrade polysaccharides such as mannan widely present in bean seeds into oligosaccharides such as mannan oligosaccharides, which not only eliminates the anti-nutritional effect of mannan on various nutrients of monogastric animals, but also The resulting mannooligosaccharides play an important regulatory r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N9/42A23K1/165C12R1/685A23K20/189
Inventor 许尧兴王有良李艳丽许少春柳永
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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