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Red tea fungus beverage produced by mixed culture fermentation of pure fungus

A technology of mixed fermentation and kombucha, applied in the field of microorganisms, to achieve the effects of stabilizing product quality, ensuring product safety, and avoiding contamination by miscellaneous bacteria

Inactive Publication Date: 2012-06-06
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The notable advantage of the present invention is: the present invention through research to functional kombucha beverage: at first must separate and obtain the pure strain of kombucha, and understand the taxonomic position of strain, just can solve strain storage time, inoculum quantity control, prevent pollution, It is possible to conduct in-depth and detailed research on the various metabolic characteristics, culture conditions, fermentation broth components and possible functional effects of each strain and their various combinations, such as maintaining the vitality of the strain and controlling the quality of the product. , through self-isolation and screening to obtain three pure strains: Saccharomyces cerevisiae Meyen ex Hansen, Acetobacter aceti and Lactobacillus plantarum

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 2

[0026] (1) Seed culture: pick the activated Saccharomyces cerevisiae 2-3 loops into the liquid culture medium, place it at 30°C, shake culture at 100r / min for 32h, the liquid culture medium formula of the Saccharomyces cerevisiae is: yeast extract 1g, peptone 1g, glucose 2g, water 100mL; pick the activated Acetobacter 2-3 loops into the liquid culture medium, place it at 30°C, shake culture at 200r / min for 32h, the liquid culture of Acetobacter acetate The basic formula is: 1g yeast extract, 1g glucose, 100mL water, and 3mL absolute ethanol; pick the activated Lactobacillus plantarum 2-3 loops into MRS liquid medium, place it at 30℃, and incubate it for 32h. The formula of the MRS liquid medium is: peptone 1g, beef extract 1g, yeast extract 0.5g, glucose 2g, sodium acetate 0.5g, Tween 800.1mL, citrate diamine 0.2g, MgSO 4 ·7H 2 O 0.2g, MnSO 4 ·4H 2 O 0.005g, K 2 HPO 4 0.2g, 100mL water, pH 6.2-6.6;

[0027] (2) Kombucha fermentation: According to the ratio of Saccharomyces cerev...

Embodiment 3

[0029] (1) Seed culture: Pick up the activated Saccharomyces cerevisiae 2-3 loops and connect it to liquid culture medium, place it at 30°C, and culture with shaking at 100r / min for 34 hours. The liquid culture medium formula of Saccharomyces cerevisiae is: yeast extract 1g, peptone 1g, glucose 2g, water 100mL; pick the activated Acetobacter 2-3 loops into the liquid medium, place it at 30°C, 200r / min shaking culture for 35h, the liquid culture of Acetobacter acetate The basic formula is: yeast extract 1g, glucose 1g, water 100mL, and absolute ethanol 3mL; pick the activated Lactobacillus plantarum 2-3 loops into the MRS liquid medium, place it at 30℃, and cultivate it for 35h. The formula of the MRS liquid medium is: peptone 1g, beef extract 1g, yeast extract 0.5g, glucose 2g, sodium acetate 0.5g, Tween 800.1mL, citrate diamine 0.2g, MgSO 4 ·7H 2 O 0.2g, MnSO 4 ·4H 2 O 0.005g, K 2 HPO 4 0.2g, 100mL water, pH 6.2-6.6;

[0030] (2) Kombucha fermentation: According to the weight r...

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PUM

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Abstract

The invention provides a black tea fungus drink which is produced by the mixed fermentation of pure fungi. Three kinds of fungi of saccharomyces cerivisiae, bacillus aceticus and lactobacillus plantarum are compounded, and mixed and fermented to produce the black tea fungus drink. In the drink, the combination of the pure fungi and the mixed fermentation are utilized, and the formula of the culture medium of the black tea fungus and the fermentation process conditions are optimized, thereby obviously shortening the fermentation time, avoiding the pollution of mixed fungi and ensuring the product safety; management methods for culture, rejuvenation and preservation of the fungus are scientific and reasonable, the product quality is stable, and a good basis is laid for the industrialized production of the black tea fungus; and the prepared black tea fungus drink not only tastes good, but also contains various effective components, and is a microbial fermentation drink with high utility value.

Description

Technical field [0001] The invention belongs to the technical field of microorganisms, and more specifically relates to a kombucha beverage produced by mixed fermentation of pure bacteria. Background technique [0002] With the development of science and technology and the improvement of people's material living standards, many people have become more aware of the interrelationship between disease, health and food, and once again put forward the viewpoint of diet therapy: improve dietary conditions and food composition, and make use of food itself Physiological adjustment function to achieve the purpose of improving human health. Based on this consumption concept, people are paying more and more attention to physical health and the pursuit of beverage quality. Kombucha is a symbiosis system composed of three kinds of probiotics that are beneficial to human health, namely yeast, acetic acid bacteria, and lactic acid bacteria. Through its own special effects, tea and other raw mat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23L2/38A23L1/28C12N1/20A23L1/29A23L31/10
Inventor 林娟刘树滔饶平凡
Owner FUZHOU UNIV
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