Red tea fungus beverage produced by mixed culture fermentation of pure fungus
A technology of mixed fermentation and kombucha, applied in the field of microorganisms, to achieve the effects of stabilizing product quality, ensuring product safety, and avoiding contamination by miscellaneous bacteria
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Embodiment 2
[0026] (1) Seed culture: pick the activated Saccharomyces cerevisiae 2-3 loops into the liquid culture medium, place it at 30°C, shake culture at 100r / min for 32h, the liquid culture medium formula of the Saccharomyces cerevisiae is: yeast extract 1g, peptone 1g, glucose 2g, water 100mL; pick the activated Acetobacter 2-3 loops into the liquid culture medium, place it at 30°C, shake culture at 200r / min for 32h, the liquid culture of Acetobacter acetate The basic formula is: 1g yeast extract, 1g glucose, 100mL water, and 3mL absolute ethanol; pick the activated Lactobacillus plantarum 2-3 loops into MRS liquid medium, place it at 30℃, and incubate it for 32h. The formula of the MRS liquid medium is: peptone 1g, beef extract 1g, yeast extract 0.5g, glucose 2g, sodium acetate 0.5g, Tween 800.1mL, citrate diamine 0.2g, MgSO 4 ·7H 2 O 0.2g, MnSO 4 ·4H 2 O 0.005g, K 2 HPO 4 0.2g, 100mL water, pH 6.2-6.6;
[0027] (2) Kombucha fermentation: According to the ratio of Saccharomyces cerev...
Embodiment 3
[0029] (1) Seed culture: Pick up the activated Saccharomyces cerevisiae 2-3 loops and connect it to liquid culture medium, place it at 30°C, and culture with shaking at 100r / min for 34 hours. The liquid culture medium formula of Saccharomyces cerevisiae is: yeast extract 1g, peptone 1g, glucose 2g, water 100mL; pick the activated Acetobacter 2-3 loops into the liquid medium, place it at 30°C, 200r / min shaking culture for 35h, the liquid culture of Acetobacter acetate The basic formula is: yeast extract 1g, glucose 1g, water 100mL, and absolute ethanol 3mL; pick the activated Lactobacillus plantarum 2-3 loops into the MRS liquid medium, place it at 30℃, and cultivate it for 35h. The formula of the MRS liquid medium is: peptone 1g, beef extract 1g, yeast extract 0.5g, glucose 2g, sodium acetate 0.5g, Tween 800.1mL, citrate diamine 0.2g, MgSO 4 ·7H 2 O 0.2g, MnSO 4 ·4H 2 O 0.005g, K 2 HPO 4 0.2g, 100mL water, pH 6.2-6.6;
[0030] (2) Kombucha fermentation: According to the weight r...
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