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Hedyotis diffusa Willd. extract and method for separating and preparing the same

A technology for extracting Hedyotis diffusa, which is applied in the field of separation and preparation of Hedyotis diffusa extract, can solve the problems of inability to produce on a large scale, outdated detection methods, and low degree of automation, etc., and is suitable for large-scale industrial production, Small organic solvent residue and good reproducibility

Inactive Publication Date: 2009-08-05
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Iridoids are one of the main active ingredients in Hedyotis diffusa, and currently reported methods for the separation and preparation of iridoids basically adopt traditional methods such as solvent extraction and silica gel column chromatography. The method has disadvantages such as poor reproducibility, long time-consuming, serious organic residues, backward detection methods, low degree of automation, and incapable of large-scale production.

Method used

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  • Hedyotis diffusa Willd. extract and method for separating and preparing the same
  • Hedyotis diffusa Willd. extract and method for separating and preparing the same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Grind the medicinal material of Hedyotis diffusa, weigh 2 kg quantitatively, put it in a 20-liter extraction tank, add 20 liters of water to decoct for 1 hour, filter, and save the filtrate for later use, add 20 liters of water to the filter residue and decoct for 1 hour, filter, and the filtrate Combined with the first time, the filter residue was discarded. The extract was concentrated by rotary evaporation to 500 ml to obtain the water extract fraction of Hedyotis diffusa. Add 710 milliliters of 95% ethanol to the concentrated solution, stir well, let stand at room temperature for 24 hours, filter, discard the filter residue, concentrate the filtrate to 300 milliliters, add 1600 milliliters of 95% ethanol to the concentrated solution, stir well, and let stand at room temperature for 24 hours hour, filtered, and the filter residue was discarded, the filtrate was concentrated until it had no alcohol smell, and the concentrated solution was centrifuged in a high-speed c...

Embodiment 2

[0031] Grind the medicinal material of Hedyotis diffusa, weigh 5 kg quantitatively, put it in a 100-liter extraction tank, add 40 liters of water to decoct for 2 hours, filter, and save the filtrate for later use, add 40 liters of water to the filter residue and decoct for 2 hours, filter, and the filtrate Combined with the first time, the filter residue was discarded. The extract was concentrated by rotary evaporation to 1000 ml to obtain the water extract fraction of Hedyotis diffusa. Add 1710 milliliters of 95% ethanol to the concentrated solution, stir well, let stand at room temperature for 24 hours, filter, discard the filter residue, concentrate the filtrate to 500 milliliters, add 2670 milliliters of 95% ethanol to the concentrated solution, stir well, and let stand at room temperature for 24 hours hour, filtered, and the filter residue was discarded, the filtrate was concentrated until it had no alcohol smell, and the concentrated solution was centrifuged in a high-sp...

Embodiment 3

[0035] Grind the medicinal material of Hedyotis diffusa, weigh 10 kg quantitatively, put it in a 100-liter extraction tank, add 60 liters of water to decoct for 3 hours, filter, and save the filtrate for later use, add 60 liters of water to the filter residue and decoct for 3 hours, filter, and the filtrate Combined with the first time, the filter residue was discarded. The extract was concentrated by rotary evaporation to 2000 ml to obtain the water extract fraction of Hedyotis diffusa. Add 3420 milliliters of 95% ethanol to the concentrated solution, stir well, let stand at room temperature for 24 hours, filter, discard the filter residue, concentrate the filtrate to 1500 milliliters, add 8000 milliliters of 95% ethanol to the concentrated solution, stir well, and let stand at room temperature for 24 hours hour, filtered, and the filter residue was discarded, the filtrate was concentrated until it had no alcohol smell, and the concentrated solution was centrifuged in a high-sp...

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Abstract

The invention relates to a spreading hedyotis herb extract and a separation and preparation method thereof. The main components of the extract are paederoside methyl ester, deacetylasperulosidic acid methyl ester, asperulosidic acid and asperuloside, and the total content of the four main components is 60 to 75 percent. The extract is obtained from the spreading hedyotis herb through steps of extracting, depositing in alcohol, membrane separation, non-polar macroporous resin separation, industrial chromatographic resolution, and the like. The preparation method can effectively remove the impurities such as glycoprotein, polysaccharide, amino acid, and the like, and improve the content of the main active components. The preparation process has high repeatability and good operability, is easy to realize standardization and industrialization, and simultaneously has certain directive significance for the quality control of the spreading hedyotis herb.

Description

technical field [0001] The present invention relates to the separation of natural medicines, specifically a method for the separation and preparation of Hedyotis diffusa extract. The main components of the component are gallinacein methyl ester, deacetylated cartilinic acid methyl ester, carrionidic acid and cartilin four compounds. Background technique [0002] The traditional Chinese medicine Hedyotis diffusa is the whole plant of Hedyotis diffusaWilld., also known as Oldenlandia diffusa Roxb. First recorded in "Guangxi Traditional Chinese Medicine", it has the functions of clearing heat and detoxifying, promoting blood circulation and removing blood stasis, diuresis and dampness, reducing swelling and pain, and anti-tumor. It is widely used in the treatment of tumors, icteric hepatitis, enteritis and pneumonia. Mainly produced in Guangdong, Guangxi, Fujian and other places. The main components of Hedyotis diffusa are iridoids, flavonoids and anthraquinones, followed by ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/74A61P1/00A61P1/16A61P11/00A61P31/14A61P35/00
Inventor 梁鑫淼李存满丰加涛薛兴亚章飞芳徐青
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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