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Method for extracting plant source type fluorochrome

A technology of fluorescent pigment and extraction method, which is applied in the field of extraction of plant pigment, can solve the problem that the technology of fluorescent substance has not been reported, and achieves the effect of high application value

Inactive Publication Date: 2009-08-19
SUZHOU UNIV
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  • Abstract
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Problems solved by technology

[0003] At present, there is no report on the technology of extracting fluorescent substances with high yield and high purity from plants

Method used

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Experimental program
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Embodiment 1

[0017] The technical solution of this embodiment is suitable for using the petals of Rhododendron as raw material to extract natural fluorescent pigment therefrom, and the specific steps are as follows:

[0018] (1) Weigh 100g of dried rhododendron petals, put them in a 3L beaker, add 1000mL of formic acid solution with a mass concentration of 10%, and extract at a low temperature of 4-7°C for 12 hours; filter the extract with No. 1 filter paper , Add 1000mL of 10% formic acid to the filter residue, and filter it with No. 1 filter paper after leaching at room temperature for 4 to 6 hours.

[0019] (2) Merge the filtrate twice, and dilute with 2000mL of distilled water, add 4000mL of diluent in the macroporous adsorption resin HP-20 column, the column volume is 5cm×40cm;

[0020] (3) After washing the column with 8000 mL of distilled water, use 1500 mL of eluent 1 (formic acid: ethanol volume ratio is 5:95) to elute the anthocyanidins in the column;

[0021] (4) Collect the an...

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PUM

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Abstract

The invention discloses an extracting method of phytogenous fluorchrome, which extracts the fluorchrome from plant petals, comprising the steps: putting fresh flowers into 10% formic acid, leaching for 12-16 h, and filtering; filtering the filtrate in a macropore sephadex column after the filtrate is diluted by distilled water; eluting pigment in the column by eluent 1; collecting the pigment liquid, and concentrating at reduced pressure; filtrating the concentrated pigment liquid in a micropore sephadex column after the concentrated pigment is dissolveed by 10% formic acid; eluting the pigment in the column by eluent 2; and collecting the eluate with fluorescent characteristic under ultraviolet light to obtain the phytogenous fluorchrome. The invention extracts natural fluorescent material with high yield and high purity so as to provide novel material for developing fluorescent food additive and have application value for printing the invisible and the anti-fake marks, etc.

Description

technical field [0001] The invention relates to a method for extracting plant pigments, in particular to a method for extracting fluorescent pigments in plant petals. Background technique [0002] Plant-derived fluorescent pigments refer to heteropolycyclic compounds or flavonol compounds in plants, which can emit fluorescence after being irradiated by ultraviolet light. It is different from chemically synthesized fluorescent dyes or pigments, and belongs to organic substances of plant secondary metabolism. , non-toxic to the human body, no radiation. According to the luminescent characteristics of these organic substances, fluorescence can be divided into two types: autofluorescence and induced fluorescence. The former, such as chlorophyll and other heteropolycyclic rings, emit red fluorescence after being irradiated by ultraviolet rays; After ultraviolet irradiation, it emits blue and yellow fluorescence. The petals of many ornamental plants contain not only some anthocy...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09B67/54C09B61/00
Inventor 陆小平李叶峰刘晓红徐剑周君王勤
Owner SUZHOU UNIV
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