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Method and device for collecting microalgae utilizing phototropism

A microalgae body and phototaxis technology is applied in the field of phototaxis microalgae harvesting devices, which can solve the problems of high cost, unenvironmental protection, high energy consumption and the like, and achieve the effects of no need for later investment, convenient operation and high efficiency.

Inactive Publication Date: 2009-09-02
新奥天然气股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] Although the above-mentioned existing technical solutions can collect algae, there are some disadvantages in these existing technologies. For example, the use of centrifugation to process microalgae culture fluid requires expensive centrifuges, and the price is usually hundreds of thousands to millions. , high equipment investment cost and high energy consumption; the membrane filtration method has the disadvantages of serious membrane pollution, difficult cleaning, and high cost; although the flocculation method is relatively simple to operate, flocculants such as aluminum potassium sulfate and additives such as polyacrylamide need to be washed and removed , time-consuming, and high cost; the air flotation method needs to increase gas supply equipment and gas, which is costly and not environmentally friendly
In addition, the collection of algae by these collection methods is discontinuous and separated from the cell culture process, resulting in a lack of continuity and unity in the production process, which greatly reduces the comprehensive utilization efficiency of production equipment

Method used

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  • Method and device for collecting microalgae utilizing phototropism
  • Method and device for collecting microalgae utilizing phototropism

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] In a 100ml volumetric flask, fill 100ml of Dunaliella salina culture solution in the late stage of growth, algae OD 750 0.8, the volumetric flask was protected from light, incubated at 25°C, and a small hole with a diameter of 0.5 cm was opened on the lower side of the light-proof layer of the volumetric flask, and 5000 Lux of light penetrated through the hole. After 24 hours, the same Compared with the control group collected without light, the algae OD 750 0.044, 99% of the Dunaliella salina in the volumetric flask gathered around the light-transmitting opening, poured out the supernatant, and the lower layer was the collected high-concentration algae liquid.

Embodiment 2

[0045] In a 100ml volumetric flask, 100ml of Dunaliella salina culture solution in the late stage of growth is installed, and the algal body OD 750 is 0.8. Protect the volumetric flask from light and incubate at 25°C. Open a small hole with a diameter of 0.5 cm at the bottom of the light-shielding layer of the volumetric flask that is protected from light. 5000 Lux of light penetrates through this hole. After 24 hours, compare with the one collected without light Compared with the group, almost all Dunaliella salina in the volumetric flask gathered around the light-transmitting opening, the supernatant was poured out, and the lower layer was the collected high-concentration algae liquid. OD of supernatant medium 750 is 0.07, and the recovery rate is about 97%.

Embodiment 3

[0047] Fill 50L of Dunaliella salina UTEX999 culture solution cultivated to the logarithmic phase in a 50L columnar culture vessel (the bottom is a light-transmitting layer), and the OD of the algae 750 is 0.8. Shade the incubator, open a light hole on the lower side of the light-shielding layer of the incubator, place a white point light source, the diameter of the light hole is 5cm, the light intensity is 10000Lux, the collection temperature is 25°C, most of the Dunaliella salina gathers around the light source, and the supernatant is pumped out with a water pump. The lower layer is the collected high-concentration algae liquid, and the supernatant algae body OD 750 It is about 0.09, and the recovery rate of algae body is about 90%.

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Abstract

The invention provides a method for collecting microalgae utilizing phototropism of the microalgae, comprising the steps of cultivating the microalgae in a microalgae cultivating device under the condition suitable for the growth of the microalgae until the microalgae to be collected grows to a needed collectable phase, shielding all transparent surfaces of the microalgae cultivating device or collecting device with a light shield layer, disposing one or more light holes on the light shield layer of any transparent surface, placing one or more light sources at the light hole(s), removing clear solution when the microalgae in the culture fluid basically gathers around the light source after a proper time, and obtaining microalgae with high concentration. The invention also provides the phototactic microalgae collecting device which is formed by simple alteration.

Description

technical field [0001] The invention provides a method for harvesting algae bodies by utilizing the phototaxis of microalgae. The invention also provides a phototaxis microalgae harvesting device which is simply modified. Background technique [0002] Algae are the lowest, self-supporting oxygen-evolving plants, and they are a group of various and extremely widely distributed lower plants. Whether it is oceans, freshwater lakes and other waters, or wet soil, tree trunks, etc., it can survive almost anywhere with light and humidity. Microalgae refer to some microscopic unicellular, group or filamentous algae, most of which are planktonic algae with large biomass and wide distribution. Algae can obtain biomass fuel through pyrolysis, which is an important renewable bioenergy source; through agrochemical technology, oil can be extracted from some fat-rich microalgae for the preparation of edible oil and biodiesel; algae also contains a variety of Vitamins, carotene, protein,...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12M1/26C12R1/89
CPCC12M21/02C12M31/10C12M23/22C12M33/00C12M23/08
Inventor 吴洪袁普卫蔡忠贞尹顺吉王媛媛邓平
Owner 新奥天然气股份有限公司
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