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Method for quickly detecting content of fatty acid in marine microalgae

A marine microalgae and fatty acid technology, applied in the field of marine microorganisms, can solve problems such as unsuitable for rapid detection, long operation time, cumbersome and other problems, and achieve the effects of intuitive data, less amount of organic reagents, and simple operation steps

Inactive Publication Date: 2009-09-16
THE FIRST INST OF OCEANOGRAPHY SOA
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Problems solved by technology

Compared with other methods, although the operation steps of this method have been simplified, it is still relatively cumbersome and takes a long time, so it is not suitable for rapid detection in the laboratory

Method used

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  • Method for quickly detecting content of fatty acid in marine microalgae

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Embodiment Construction

[0037] Protection scope of the present invention is not only limited in the following examples, see figure 1 Concrete technical steps of the present invention are as follows:

[0038] Chromatographic column: HP-5MS quartz capillary column (30m×0.25mm id×0.25um); carrier gas: helium, flow rate 1mL / min, inlet temperature 250°C, transfer line temperature 300°C. The electron energy is 70eV, the ion source temperature is 230°C, and the quadrupole temperature is 150°C. The heating program is as follows: the initial temperature is 50°C, and the temperature is increased to 280°C at 10°C / min. The injection volume was 1 μL, split injection (10:1), and the scanning mode was full scan (Scan).

[0039] 4. Identification of fatty acids:

[0040] With reference to standard samples and spectral library NIST5.0, the percentage content of fatty acid components was calculated by normalization method, expressed as a percentage of the total amount of fatty acids.

[0041] 5. Verification of ac...

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Abstract

The invention provides a method for quickly detecting content of fatty acid in marine microalgae, which extracts and saponifies fat in the microalgae by saturated KOH-CH3O, integrates the extraction process and the saponification process into one step, performs methyl esterification by HCl-CH3OH to obtain the fatty acid, performs gas chromatographic mass spectrometric analysis on the fatty acid after extraction, saponification and the methyl esterification, and comprises the following steps: (1) sample preparation, (2) extraction, saponification and methyl esterification of the fatty acid, and(3) gas chromatography detecting condition for the fatty acid. The method has the advantages of simple operation steps, time conservation, simple and quick method, visual data, and increase of the comparability between samples.

Description

technical field [0001] The invention belongs to the field of marine microbes, specifically, it utilizes saturated KOH-CH to marine microalgae fatty acids 3 OH method for rapid detection of fatty acid content in marine microalgae. Background technique [0002] Marine microalgae are rich in fatty acids, polyunsaturated fatty acids (PUFAs), proteins, various vitamins, biological polysaccharides, etc., and have excellent nutritional and health functions. The development and utilization of microalgae resources has become one of the focuses in the field of life science and technology in the world today. [0003] Studies have confirmed that polyunsaturated fatty acids are important for organisms to transform into metabolites that regulate certain physiological functions. γ-linolenic acid (γ-linolenic acid), AA, eicosapentaenoic acid [20:5(n-3), EPA] and docosahexaenoic acid [22:6(n-3), DHA ] are relatively important PUFAs, of which EPA and DHA are essential fatty acids for the h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N1/28
Inventor 杨佰娟郑立韩笑天刘涛
Owner THE FIRST INST OF OCEANOGRAPHY SOA
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