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Separation and purification technology of trehalose by one-step method

A technology of separation and purification, trehalose, applied in the directions of disaccharides, sugar derivatives, sugar derivatives, etc., can solve the problems of many separation and purification steps, low target products, high product prices, etc., to reduce production costs and reduce separation and purification. effect of steps

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Problems solved by technology

A domestic company has already used the enzymatic conversion method to produce trehalose, but it is restricted by the raw material - cassava starch planting; while the traditional yeast extraction method has many separation and purification steps, resulting in high production costs, low target products, and high product prices. Limit the wide application of trehalose

Method used

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  • Separation and purification technology of trehalose by one-step method

Examples

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Effect test

Embodiment 1

[0013] Take 5g of dry yeast and add 100ml of 80% ethanol solution, and stir and extract for 2 hours in the range of 80°C. Add 3 g of zinc sulfate to the extract, adjust the pH value to neutral with sodium hydroxide, filter with a plate and frame filter, and the obtained clarified filtrate is the purified trehalose solution.

Embodiment 2

[0015] Take 5g of dry yeast and add 100ml of water, and stir and extract at 40°C for 2 hours. Add 5 g of zinc sulfate to the extract, adjust the pH value to neutral with calcium oxide, filter with a plate and frame filter, and the obtained clarified filtrate is the purified trehalose solution.

Embodiment 3

[0017] Take 10g of dry yeast and add 100ml of 50% ethanol solution, and stir and extract for 2 hours at 50°C. Add 4 g of zinc sulfate to the extract, adjust the pH value to neutral with sodium hydroxide, filter with a plate and frame filter, and the obtained clarified filtrate is the purified trehalose solution.

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Abstract

The invention relates to an extraction, separation and purification method of trehalose, in particular to a separation and purification method of trehalose by one-step. The method comprises the steps of: after extracting dry yeast by using water, adding a flocculating agent to adjust the pH value; and then filtering to obtain clear filtrate, and directly concentrating and crystallizing to obtain trehalose crystals. The method has simple separation and purification steps, convenience and feasibility, low cost and good effect, and provides conditions for the trehalose to be widely used in the fields of medicine, cosmetics, food preservation and the like.

Description

technical field [0001] The invention relates to a technology for separating and purifying trehalose from dry yeast. Background technique [0002] Trehalose, known as the "sugar of life", is non-toxic and harmless, has no side effects on the human body, and its basic properties are similar to other disaccharides. However, it has a non-specific protective effect, which can protect the dried and dehydrated organisms in the form of extremely low or even stopped metabolism. Once the environment permits, the organisms can be revived without damaging the living substances. Therefore, trehalose is widely used. Used in dry preservation of food, medicine, hygiene, beauty and so on. Currently, trehalose is produced worldwide mainly by enzymatic conversion and extraction from yeast strains. A domestic company has used the enzymatic conversion method to produce trehalose, but it is restricted by the raw material - cassava starch planting; while the traditional yeast extraction method h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H3/04C07H1/06
Inventor 张丽杰赵天涛全学军张云茹
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