Check patentability & draft patents in minutes with Patsnap Eureka AI!

Signal peptide, coding genes thereof and application

A technology of coding and fusion genes, which is applied in application, genetic engineering, plant gene improvement, etc., can solve the problems of low expression of target protein and inactive expression products, and achieve good biological activity, high protein yield, and simple purification process Effect

Inactive Publication Date: 2009-09-23
INST OF BIOENG ACAD OF MILITARY MEDICAL SCI OF THE CHINESE
View PDF0 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For some proteins with a special structure at the N-terminus, the use of α-mating factors often leads to low expression of the target protein or no activity of the expression product

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Signal peptide, coding genes thereof and application
  • Signal peptide, coding genes thereof and application
  • Signal peptide, coding genes thereof and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1, the pre peptide of α-mating factor as a secretion signal

[0026] 1) Acquisition of gONC and agONC genes

[0027] A series of primers were designed and synthesized according to the coding sequence of the Onconase gene in Genebank and yeast preferred codons. The nucleotide sequences of the primers are as follows:

[0028] Primer 5: 5'GCCTCGAGAAAAGACAAGACTGGTTGACCTTCCAAAAGAAG3';

[0029] Primer 3: 5'CTGAATTCCTATTAACAACTACCGACGCCAACGAAGTG3';

[0030]P1: 5'CAAGACTGGTTGACCTTCCAAAAGAAGCACATCACCAACACCAGAGACGTCGACTGTG3';

[0031] P2: 5'ACCAACACCAGAGACGTCGACTGTGACAACATCATGTCCACCAACTTGTTCCACTGTA3';

[0032] P3: 5'ATGTCCACCAACTTGTTCCACTGTAAGGACAAGAACACCTTCATCTACTCCCCGTCCAG3';

[0033] P4: 5'AACACCTTCATCTACTCCCGTCCAGAGCCAGTCAAGGCTATCTGCAAGGGTATCATCG3';

[0034] P5: 5'AAGGCTATCTGCAAGGGTATCATCGCTTCCAAGAACGTCCTTACCACCTCCGAGTTCT 3';

[0035] P6: 5'AACGTCCTTACCACCTCCGAGTTCTACTTGTCCGACTGTAACGTTACCAGTAGACCAT3';

[0036] P7: 5'GACTGTAACGTTACCAGTAGACCATGCAAGTACAAGTTGAAGAAG...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a signal peptide, coding genes thereof and application. The amino acid sequence of the signal peptide is sequence 1 in a sequence table. The invention also discloses DNA molecule of coding the peptide. The nucleotide sequence of the DNA molecule is sequence 2 in the sequence table. The peptide and the DNA molecule can be applied in the protein production. The invention also discloses a method for producing protein, which is as follows: the DNA molecule is fused with target genes to obtain fusion gene which are led into yeast so as to obtain recombinant yeast which is fermented so as to produce target protein. Pre peptide of alpha-mating factors of the invention and the coding genes thereof can be used for producing the protein, the N end of which has special construction; the protein yield is high; the purge process is simple; the invention has good biological activity.

Description

technical field [0001] The invention relates to a signal peptide, its coding gene and application. Background technique [0002] Due to the presence of ribonuclease inhibitors, the level of ribonuclease degrading RNA in cells will not affect the survival of cells; however, in recent years, more and more cytotoxic ribonucleases have been discovered, including Angrogenin, bovine semen ribose nuclease, etc. [0003] Onconase (hereinafter referred to as ONC), translated as Leopard Frog Enzyme in Chinese, was first isolated from oocytes and early embryos of Rana pipiens (Arctic Leopard Frog), and belongs to the ribonuclease A superfamily (RNase A). In 1988, Mikulski et al. found that the purified protein (p-30) and its crude extract (Pannon) could effectively inhibit human leukemia cell line HL-60, human jaw cancer cell line A-253 and human adenocarcinoma cell line Colo 320 in vitro. The growth and killing of these cells by CM etc. showed a time-dose-dependent effect, and the i...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07K7/08C12N15/31C12N15/62C12N15/63C12N5/10C12N1/00C12N15/11C12P21/02C12R1/84
Inventor 刘志敏何庆赵洪亮薛冲
Owner INST OF BIOENG ACAD OF MILITARY MEDICAL SCI OF THE CHINESE
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com