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Extraction method of fish skin collagen

A technology of fish skin collagen and fish collagen, which is applied in the fields of pharmaceutical formula, food preparation, food science, etc., to achieve the effects of low cost, solving environmental pollution and safety hazards, and short production cycle

Inactive Publication Date: 2009-09-23
湖北瑞邦生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no report on the technology of degreasing and extracting collagen in one step using biological enzymatic digestion technology

Method used

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  • Extraction method of fish skin collagen

Examples

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Effect test

Embodiment 1

[0024] Accurately weigh and remove impurities, wash 1 kg of fresh fish skin, add 2 kg of distilled water, and stir well. Heat the suspension to 35°C-70°C, adjust the pH to 9 with calcium hydroxide, add 10 grams of triacylglycerol lipase, and the enzymatic hydrolysis time is 2 hours, then add 12 grams of alkaline protease, and the enzymatic hydrolysis time is After 4 hours, the temperature was raised to 95°C and kept for 10 minutes to inactivate the enzyme, and then the enzymolysis solution was filtered to obtain the supernatant and filter residue, and the supernatant was concentrated and dried to obtain fish skin collagen.

Embodiment 2

[0026] Accurately weigh and remove impurities, wash 2 kg of fresh fish skin, add 3 kg of distilled water, and stir evenly. Heat the suspension to 35°C-70°C, adjust the pH to 10 with calcium hydroxide, add 15 grams of diacylglycerol lipase, and the enzymolysis time is 1.5 hours, then add 20 grams of papain, and the enzymolysis time is 5 hours. hours, then raise the temperature to 95°C and keep for 10 minutes to inactivate the enzyme, then filter the enzymolysis solution to obtain supernatant and filter residue, the supernatant is concentrated and dried to obtain fish skin collagen.

Embodiment 3

[0028] Accurately weigh and remove impurities, wash 5 kg of fresh fish skin, add 8 kg of distilled water, and stir evenly until the fish scale powder becomes a suspension. Heat the suspension to 35°C-70°C, adjust the pH to 8 with calcium hydroxide, add 20 grams of monoacylglycerol lipase, and the enzymatic hydrolysis time is 1.8 hours, then add 30 grams of trypsin, and the enzymatic hydrolysis time is 4 hours hours, then raise the temperature to 95°C and keep for 10 minutes to inactivate the enzyme, then filter the enzymolysis solution to obtain supernatant and filter residue, the supernatant is concentrated and dried to obtain fish skin collagen.

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Abstract

The invention takes fish skin as a raw material and uses biological enzyme digestion technology to degrease as well as to extract fish collagen from the fish skin. The extraction of the fish collagen includes the steps of: decontamination of the fish skin, cleaning, water distribution, degreasing by lipase, then zymohydrolysis by prolease, enzyme killing and filtration, and the concentration and drying of obtained filtrate, thus finally obtaining the fish collagen.

Description

[0001] (1) A kind of extraction method of fish skin collagen (2) Technical field: [0002] The invention relates to a deep processing technology of fish skin, in particular to a method of using fish skin as a raw material, degreasing by bioengineering enzymatic cutting technology and extracting fish skin collagen by using biological enzymatic cutting technology. (3) background technology: [0003] The main components in fish skin are fat and collagen. Collagen is an important functional protein, which is closely related to cell proliferation, differentiation, exercise, immunity, joint lubrication, wound healing, etc. Modern research shows that collagen has the functions of increasing protein nutrition, preventing osteoporosis, anti-oxidation, delaying aging, beautifying, and lowering blood pressure. The main source of raw materials for collagen products currently produced is the skin, bone and tendons of terrestrial mammals such as pigs and cattle. In recent years, with th...

Claims

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Application Information

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IPC IPC(8): C12P21/06A23L1/305A61K8/65A23L33/17
Inventor 凌海军
Owner 湖北瑞邦生物科技有限公司
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