Antioxidative substance and composition and use thereof

A technology of antioxidant substances and cosmetic compositions, applied in the field of medicine, can solve the problems of anti-oxidation and anti-aging of white fresh extracts that have not been reported in literature

Active Publication Date: 2009-09-30
XIANGBEI WELMAN PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] Therefore, so far, there has been no literature report that

Method used

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  • Antioxidative substance and composition and use thereof
  • Antioxidative substance and composition and use thereof
  • Antioxidative substance and composition and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0079] Example 1 Extract preparation

[0080] Preparation of Radix Cortex Extract

[0081] Use 50L of 70% ethanol to carry out alcohol extraction on 5Kg of the pulverized powder of Cortex Cortex; reduce the above-mentioned alcohol extract to recover the solvent to a minimum; dry it under reduced pressure to dryness, and then obtain the extract of Cortex Cortex APD-002;

[0082] Preparation of Total Alkaloid Extract from Cortex Dictica

[0083] Use 50L pH2.0 hydrochloric acid aqueous solution to carry out acid water leaching to the crushed powder of 5Kg white fresh bark; then pass the above-mentioned extract solution through a cation exchange resin column; wash the cation resin adsorbed with alkaloids to neutrality; use 50% Ethanol and acetone eluted to separate the alkaloids; the eluate was dried to dryness to obtain the total alkaloids of the fresh bark APD-N2;

[0084] Qualitative experiment of alkaloid compounds: take a small amount of total alkaloids of white fresh bark ...

Embodiment 2

[0085] Example 2 Effects of Extracts on the Lifespan of C.elegans

[0086] Experimental Materials:

[0087] C.elegans (DH2) species, purchased from Institute of Molecular and Cell Biology National University of Singapore; SOD quantitative assay kit, purchased from Nanjing Jiancheng Bioengineering Company; resveratrol, purchased from Shanghai Junchuang Biotechnology Co., Ltd.; Trizol Reagent, purchased from Gibcol Company of the United States; APD-002, the extract of D. chinensis, and APD-N2, the total alkaloids of D. basilisk, were prepared by Example 1.

[0088] Experimental method: refer to the literature (Dillin A.et.al.Science, 2002, 298: 830-834. BartP.et.al Mechanisms of aging and Development, 2002, 123: 105-119) method, determine APD-002, APD - Effect of N2 on the growth lifespan of C. elegans. First, treat the adults with 50% sodium hypochlorite solution and 1M potassium hydroxide solution, collect eggs and culture them in S-medium, and after the adults develop to L1...

Embodiment 3

[0103] Example 3 The protective effect of the extract of Cortex alba on subacute aging model mice induced by D-galactose

[0104] Experimental animals: male SPF grade Kunming mice, purchased from Shanghai Slack Experimental Animal Co., Ltd., weighing 18-22g, raised in SPF grade animal room, 12h light / 12h dark, free intake of feed and water.

[0105] Experimental materials: resveratrol, purchased from Shanghai Junchuang Biotechnology Co., Ltd.; APD-002, the extract of white fresh bark, prepared in Example 1; D-galactose, purchased from Shanghai No. 2 Biochemical Reagent Factory; SOD, MDA Quantitative assay kits were purchased from Nanjing Jiancheng Bioengineering Company.

[0106] Experimental method: the mice were randomly divided into 5 groups, 10 in each group: (1) the control group was given normal saline; (2) the model group was given D-galactose at a dose of 80mg / kg; (3) the drug Group A, given a dose of D-galactose of 80mg / kg, while giving a dose of resveratrol of 20mg / ...

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Abstract

The invention belongs to the technical field of medicaments, and in particular relates to an antioxidative substance and a composition and use thereof. The invention provides an extract from dictamnus dasycarpus, which has good antioxidation, can be used for preparing antioxidative medicaments, health care foods or cosmetics, and has the advantages of obvious curative effect, low price, convenient use and the like.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to an antioxidant substance and its composition and use. Background technique [0002] Aging refers to all the physiological changes in the body that occur over time, and the extent and rate of aging varies from individual to individual and is influenced by a variety of causes. Even within an individual, aging manifests itself differently for each organ, and more specifically, the function of each organ and tissue changes over time due to changes in cellular function. For example, damage to brain nerve cells leads to cognitive decline, damage to subcutaneous fat cells leads to loss of skin elasticity, loss of the ability of hair root melanocytes to produce melanin leads to gray hair, and so on. [0003] There is a clear relationship between free radicals and aging, and some scientists believe that free radicals are the main cause of aging. Oxygen free radicals generat...

Claims

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Application Information

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IPC IPC(8): A61K36/75A61K38/44A61K38/06A61K9/00A61K8/97A61K8/02A61P1/04A61P3/10A61P9/00A61P11/00A61P11/06A61P13/12A61P17/18A61P25/00A61P29/00A61P35/00A61P37/02A61P39/06A61Q19/00A23L1/30A61K31/122A61K31/355A61K31/375A61K31/661A61K31/685A61K125/00A23L33/105
Inventor 俞强
Owner XIANGBEI WELMAN PHARMA CO LTD
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