Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Function of small RNA molecule aiming at c-Jun amino terminal kinase of human for senile dementia

An amino-terminal, c-jun technology, applied in the biological field, can solve the problems of no research on the effect, prompting the acceleration of the APP metabolic process, etc.

Inactive Publication Date: 2009-10-07
SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are also reports in the literature that after stimulation with hydrogen peroxide, the amount of Aβ in cells increases, while the amount of APP and C99 decreases, suggesting that the entire metabolic process of APP is accelerated.
In short, the reason why hydrogen peroxide causes Aβ rise is not very clear, especially the role of γ-secretase, which is the last key step in limiting Aβ production, in the process of hydrogen peroxide-induced Aβ rise has not been studied

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Function of small RNA molecule aiming at c-Jun amino terminal kinase of human for senile dementia
  • Function of small RNA molecule aiming at c-Jun amino terminal kinase of human for senile dementia
  • Function of small RNA molecule aiming at c-Jun amino terminal kinase of human for senile dementia

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0081] The present invention has no special limitation on the preparation method of siRNA, including but not limited to: chemical synthesis method, in vitro transcription method and so on. It should be understood that those skilled in the art can conveniently prepare or express the siRNA in various ways after knowing the sequence of the siRNA provided by the present invention. For example, in a preferred embodiment of the present invention, the siRNA is chemically synthesized.

[0082] siRNA can be prepared in the form of a double-stranded nucleic acid, which contains a sense strand and an antisense strand, which form a duplex only under hybridization conditions. A double-stranded RNA complex can be prepared from separate sense and antisense strands. Thus, for example, complementary sense and antisense strands are chemically synthesized and subsequently hybridized by annealing to produce a synthetic double-stranded RNA complex.

[0083] The siRNA can be delivered into cells ...

Embodiment 1

[0169] Example 1. Hydrogen peroxide induces Aβ expression

[0170] Hydrogen peroxide is an oxygen free radical that exists in the body. It has the characteristics of freely permeating the cell membrane, so it has been used to simulate oxidative stress experiments in vitro. It has been reported in the literature that hydrogen peroxide can induce the increase of Aβ production, but the mechanism is not very clear. The present inventors used high concentration hydrogen peroxide to simulate the pathological state in the body of the patient to study the relationship between oxidative stress and amyloid in the pathological state.

[0171] After stimulating the cell line stably expressing APPmyc with hydrogen peroxide, the cell culture medium was collected, and the contents of Aβ40 and Aβ42 were detected with the Aβ ELISA kit (purchased from Biosource). The expression of Aβ increased ( figure 1 A).

[0172]Since the increase of Aβ40 and Aβ42 in the cell culture medium may be caused...

Embodiment 2

[0173] Example 2. Hydrogen peroxide does not cause changes in APP expression and α, β secretase activity

[0174] Therefore, it is necessary to study the reason why hydrogen peroxide promotes the increase of Aβ. Aβ is derived from the hydrolysis of its precursor protein APP protein. Therefore, any imbalance in the regulation of this metabolic process may eventually lead to abnormal deposition of Aβ. The inventors first detected the expression of the full-length protein APP protein. The results of Western blot showed that there was no significant change in the metabolism of APP itself before and after hydrogen peroxide stimulation ( figure 2 A). Including mature APP (N- and O-glycosylated APP) and immature APP (N-glycosylated APP), it is suggested that hydrogen peroxide does not affect Aβ by affecting APP synthesis and post-translational processing pathways generate.

[0175] Second, α-secretase and β-secretase can affect the production of CTF (Aβ precursor protein), there...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a purpose of an antagonist of a signal channel of c-Jun amino terminal kinase. The antagonist is used for preparing a composition for inhibiting the generation of amyloid protein. The invention also discloses a small RNA molecule used for inhibiting the generation of amyloid protein. The inventor finds for the first time that an oxidized stress agent can promote the activation of Gamma secretase by activating the signal channel of the c-Jun amino terminal kinase, thereby enabling the amyloid to be generated. Therefore, a novel medicine target spot aiming at regression neurosis can be developed on the basis of the invention.

Description

technical field [0001] The invention belongs to the field of biotechnology, and more specifically, the invention relates to the correlation between the c-Jun amino-terminal kinase (JNK) signaling pathway and the γ-secretase-dependent amyloid (Aβ) production, and the human c-Jun amino-terminal The role of small RNA molecules of kinases in Alzheimer's disease. Background technique [0002] Alzheimer's disease (AD), also known as senile dementia, is the most serious and common neurodegenerative disease in the world, and about 20 million people in the world suffer from it. Its clinical and pathological features are as follows: in the early stage, the patient's memory begins to decline, especially the short-term memory ability is significantly weakened. In the mid-term, the patient's memory further declines, life behavior becomes chaotic, and it is difficult to distinguish things around. In the late stage, the patient's personality is severely mutated, it is difficult to take c...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K38/10A61K38/16A61P25/28
Inventor 景乃禾沈承勇陈永峰
Owner SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com