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Detection of formaldehyde in urine samples

一种甲醛、尿样的技术，应用在尿样中甲醛的检测领域，能够解决费时、医疗提供者不容易获得资源或设备等问题

Inactive Publication Date: 2009-10-14
KIMBERLY CLARK WORLDWIDE INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Unfortunately, these methods have disadvantages including being time consuming and requiring specially trained professionals to perform, additionally requiring resources or equipment that are not readily available to many medical providers

Method used

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  • Detection of formaldehyde in urine samples
  • Detection of formaldehyde in urine samples
  • Detection of formaldehyde in urine samples

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0094] To determine the ability of the basic fuchsin-sulfuric acid reagent to indicate the presence of formaldehyde.

[0095] Formaldehyde aqueous solutions (mg / ml) were prepared at the following concentrations: 5.625, 11.25, 22.5, 45, 90, 180, and 360 mg / ml (corresponding to 0.056, 1.125, 2.25, 4.5, 9.0, 18.0, and 36.0%, respectively). 50 μl of formaldehyde solution of each concentration was placed in the microtiter well. In addition, water without any formaldehyde was placed in a microtiter well as a control.

[0096] In addition, 150 μl of a 2.0 mg / ml ethanol stock solution of basic fuchsin was mixed with 2.0 ml of water to prepare a basic fuchsin-sulfuric acid reagent. This produced a red solution. The mixture was then mixed slowly with 2N sulfuric acid until the red solution became colorless.

[0097] Next, 250 μl of basic fuchsin-sulfuric acid reagent was added to each microtiter well containing formaldehyde and mixed for at least about 2 minutes. Each plate was subs...

Embodiment 2

[0099] It was determined that the basic fuchsin-sulfuric acid reagent did not have the ability to indicate the presence of other aldehydes.

[0100] Aqueous solutions of aldehydes were prepared at the following concentrations: 1.0 mg / ml acetaldehyde, 15.0 mg / ml isovaleraldehyde, 15.0 mg / ml phenylacetaldehyde, and 5.0 mg / ml o-phthalaldehyde. Add 50 μl of each concentration to microtiter wells. Basic fuchsin-sulfuric acid reagent was prepared as described above and 250 [mu]l was added to each microtiter well. No significant color change was observed in any well.

Embodiment 3

[0102] Determines the ability of the basic fuchsin-sulfuric acid reagent to immobilize or coat a substrate.

[0103] exist The plates were coated with basic fuchsin-sulfuric acid reagent prepared as described above. Air dry the panel. Various concentrations of formaldehyde were prepared and applied to different parts of the board. A visible pink color change occurred in a dose-related manner at the site where the formaldehyde solution was applied.

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Abstract

A method for rapidly detecting the presence of formaldehyde in a urine sample (e.g., urine or a urinary material associated therewith, such as headspace gas located associated with urine) is provided. The method includes contacting the urine sample with a substrate on which is disposed a colorant that is capable of undergoing a detectable color change in the presence of formaldehyde. Without intending to be limited by theory, it is believed that oxidation of the colorant by formaldehyde induces either a shift of the absorption maxima towards the red end of the spectrum ('bathochromic shift') or towards the blue end of the spectrum ('hypsochromic shift'). The absorption shift provides a color difference that is detectable, either visually or through instrumentation, to indicate the presence of formaldehyde within the urine sample. For example, prior to contact with a urine sample, the colorant may be colorless or it may possess a certain color. However, after contacting the urine sample and reacting with formaldehyde, the colorant exhibits a color that is different than its initial color. The color change may thus be readily correlated to the presence of formaldehyde in the urine sample.

Description

Background of the invention [0001] Studies showing that levels of formaldehyde in urine may be indicative of genitourinary cancers such as bladder and prostate cancer, see e.g. Spanel P et al., Analysis of formaldehyde in the headspace of urine from bladder and prostate cancer patients using selected ion flow tube mass spectrometry, Rapid Commun. Mass Spectrom., 1999; 13; 1354-9. Increased formaldehyde levels may be the result of certain cancer cell types that will increase the concentration of formaldehyde in the area surrounding the cell. The presence of formaldehyde can be detected by direct testing of the urine sample or by detection of vapors on the urine sample, sometimes called headspace testing. [0002] Various methods have been developed to detect formaldehyde in urine. For example, US Patent No. 6689617 describes a multi-step process that includes a mixing step of at least 10 minutes and a reagent preparation period of at least 24 hours. Other methods including c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N31/22
CPCG01N21/78A61F13/8405G01N33/52A61F2013/8473A61F13/42G01N31/224A61F13/15Y10T436/200833Y10T436/202499G01N31/22G01N33/493
Inventor R·博加S·M·马丁M·K·史密斯K·P·麦格拉思
Owner KIMBERLY CLARK WORLDWIDE INC
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