The invention relates to a 1, 6, 7, 12-tetraphenyl perylene bisimide derivant and a preparation method thereof, belonging to the field of organic photoelectronic functional material preparation. The structural characteristic of the perylene bisimide derivant is that 1, 6, 7, 12-four harbor positions of 3, 4, 9, 10-perylene bisimide are connected with charge transport functional groups with different structure types and different conjugation degrees by phenyls. The derivant is synthesized by the following steps: firstly, a perylene bisimide core with terminal ethyne or halogen or other active groups at the periphery is synthesized by 1, 6, 7, 12-tetraphenyl perylene; and then the 1, 6, 7, 12-tetraphenyl perylene bisimide and cyclopentadienyl ketone with functional substitute group at periphery or arylamine and the like generate Diels-Alder cycloaddtion or C-N, C-C coupling reaction and the like, thus obtaining the target derivant. The compound has excellent thermal stability and morphologic stability and dissolvability; the opto-electronic property of the compound has obvious bathochromic shift compared with other perylene bisimide derivants; and the derivant can be widely applied in the fields of photoelectron and biology such as organic electroluminescence, solar cell, near-infrared fluorescence probe and the like.