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Nutrient compound based on mixed fermented culture of five probiotics

A nutritional composition and mixed fermentation technology, applied in the directions of drug combination, fermentation, medical raw materials derived from bacteria, etc., can solve the problem that a single microorganism cannot be completed, achieve high biological activity and nutritional value, and promote the effect of intestinal peristalsis.

Active Publication Date: 2009-10-21
SANZHU FUER PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in long-term experiments and production practices, people have continuously found that many important biochemical processes cannot be completed by a single microorganism or can only be carried out weakly, and must be completed by co-cultivation of two or more microorganisms

Method used

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  • Nutrient compound based on mixed fermented culture of five probiotics
  • Nutrient compound based on mixed fermented culture of five probiotics
  • Nutrient compound based on mixed fermented culture of five probiotics

Examples

Experimental program
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Effect test

Embodiment 1

[0027] Embodiment 1; The present invention is based on the preparation method of the nutritional composition of five probiotic mixed fermentation cultures

[0028] Briefly, in order to prepare the nutritional composition based on the mixed fermentation culture of five probiotic strains of the present invention, corn and oats that have been soaked and sprouted, cooked and hydrolyzed with amylase and soybeans hydrolyzed with protease can be used as raw materials to inoculate the pre-cultured Bifidobacterium bifidum and Bifidobacterium breve with approximately 0.4% (volume / volume) of each of the bacteria liquids, and reinoculated with approximately 0.4% (volume / volume) of pre-cultured Lactobacillus acidophilus after about 2 to 6 hours of conventional culture , Lactobacillus casei subsp. cheese and Lactobacillus delbrueckii subsp. bulgaricus bacteria liquid. After continuing the fermentation for about 20-22 hours, the fermentation is terminated when the pH value of the fermentatio...

Embodiment 2

[0036] Example 2: Observation of mutual antagonism and growth inhibition among the five selected probiotic bacterial strains of the present invention

[0037] According to the method described in Example 1, the nutrient medium for the mixed fermentation of five strains of probiotics is prepared, divided into 5 equal parts, and after sterilization, the pre-cultured Bifidobacterium bifidum, Bifidobacterium breve, acidophilus Lactobacillus, Lactobacillus delbrueckii, Lactobacillus casei bacteria liquid (the inoculation amount of each bacteria is 2.0% (volume / volume)). Routine culture was carried out for 24 hours, and the obtained culture was used as the sample to be tested for each strain of bacteria.

[0038] The interaction between the five strains was detected by the culture plate tube dish method (Pharmacopoeia of the People's Republic of China, 2005 edition, Appendix XI Antibiotic Microbiological Test Method).

[0039] The results are shown in Table 1 below.

[0040] Table...

Embodiment 3

[0043] Embodiment 3: the anti-killing effect of probiotic mixed fermentation culture of the present invention on some intestinal pathogenic bacteria, and the comparison with the anti-killing activity of single-strain fermentation culture

[0044] This test adopts the conventional tube-and-plate method known in the art to detect the inhibition and killing effect of the mixed fermentation culture of probiotics of the present invention on enteropathogenic bacteria E. , and observe the difference in the inhibitory effect of the five-strain mixed fermentation culture and the single probiotic fermentation culture on pathogenic bacteria.

[0045] The culture medium was prepared according to the aforementioned method and divided into six equal parts, and inoculated with the pre-cultured Bifidobacterium bifidum and Bifidobacterium breve bacteria liquid after sterilization. After 2 hours of fermentation at 38°C, inoculate Lactobacillus delbrueckii subsp. bulgaricus, Lactobacillus acidop...

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Abstract

The invention relates to a nutrient compound based on a mixed fermented culture of five probiotics, in particular to a culture prepared by using three vegetable raw materials as a fermentation substrate and mixing with of five probiotics to ferment and a preparation method thereof, a nutrient compound comprising the fermented culture and one or a plurality of medically acceptable additives and applications of the nutrient compound to the production of health foods or medicines for preventing or curing constipation and adjusting enteric flora of human bodies.

Description

technical field [0001] The present invention relates to a nutritional composition prepared by mixed fermentation culture of multiple strains of probiotics, in particular to a culture obtained through mixed fermentation of five strains of probiotics with three plant raw materials as fermentation substrates and a preparation method thereof. The nutritional composition composed of the fermented culture and one or more pharmaceutically acceptable additives, and said nutritional composition is used in the production of medicine or health food for preventing or treating constipation and regulating the intestinal flora of human body in the application. technical background [0002] Microorganisms are a class of biological resources with practical and potential uses, and are widely used in various fields such as agriculture, industry, medicine, food and environmental protection. The use of microorganisms by humans has gone through two stages: natural mixed culture and pure culture....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P1/04C12P39/00A23L1/30A61K35/74A61P1/10A23L33/135A61K35/745A61K35/747
Inventor 孙筱林吴炳新牛纪江
Owner SANZHU FUER PHARMA
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