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Method for measuring dopamine by utilizing graphite nano-sheet/Nafion composite film to modify electrode

A technology of graphite nanosheets and composite films, applied in the field of electrochemical analysis and detection, can solve problems such as the difficulty of chemically modifying electrodes, and achieve the effects of many surface active sites, simple preparation method and high sensitivity

Inactive Publication Date: 2009-11-11
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the relatively stable properties of graphite nanosheets, which are insoluble in water and general organic solvents, it is difficult to directly use them in the preparation of chemically modified electrodes.

Method used

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  • Method for measuring dopamine by utilizing graphite nano-sheet/Nafion composite film to modify electrode
  • Method for measuring dopamine by utilizing graphite nano-sheet/Nafion composite film to modify electrode

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] a. First, the glassy carbon electrode is sequentially coated with 1μm, 0.3μm, 0.05μm Al 2 o 3 The polishing powder is polished on a polishing cloth, and after each polishing, it is ultrasonically cleaned in double distilled water.

[0015] b. Prepare graphite nanosheet / Nafion mixed solution: first prepare Nafion aqueous solution, add 5% by mass Nafion solution to secondary water to dilute to obtain 0.5% by mass Nafion solution A; add graphite nanosheet to Nafion solution A to make graphite nanosheet The flake content is 7% by mass, and the black and uniformly dispersed graphite nanoflake / Nafion mixed solution B is obtained by ultrasonic shaking for 30 minutes.

[0016] c. Preparation of graphite nanosheet / Nafion composite film modified glassy carbon electrode: pipette 4 μL of the dispersion B prepared in step b with a micro-syringe and drop-coat it on the surface of the glassy carbon electrode treated in step a, and dry it at room temperature. The solvent was volatili...

Embodiment 2

[0019] a. First, the glassy carbon electrode is sequentially coated with 1μm, 0.3μm, 0.05μm Al 2 o 3 The polishing powder is polished on a polishing cloth, and after each polishing, it is ultrasonically cleaned in double distilled water.

[0020] b. Prepare graphite nanosheet / Nafion mixed solution: first prepare Nafion aqueous solution, add 5% by mass of Nafion solution to secondary water to dilute to obtain 0.9% by mass of Nafion solution A; add graphite nanosheet to Nafion solution A to make graphite nanosheet The content of flakes is 5% by mass, and the black and uniformly dispersed graphite nano flakes / Nafion mixed solution B is obtained by ultrasonic shaking for 35 minutes.

[0021] c. Preparation of graphite nanosheet / Nafion composite film modified glassy carbon electrode: pipette 6 μL of the dispersion B prepared in step b with a micro-syringe and drop-coat it on the surface of the glassy carbon electrode treated in step a, and dry it at room temperature. The solvent ...

Embodiment 3

[0024] a. First, the glassy carbon electrode is sequentially coated with 1μm, 0.3μm, 0.05μm Al 2 o 3 The polishing powder is polished on a polishing cloth, and after each polishing, it is ultrasonically cleaned in double distilled water.

[0025] b. Prepare graphite nanosheet / Nafion mixed solution: first prepare Nafion aqueous solution, add 5% by mass Nafion solution to secondary water to dilute to obtain 0.7% by mass Nafion solution A; add graphite nanosheet to Nafion solution A to make graphite nanosheet The flake content was 6% by mass, and the black and uniformly dispersed graphite nanoflake / Nafion mixed solution B was obtained by ultrasonic shaking for 35 minutes.

[0026] c. Preparation of graphite nanosheet / Nafion composite film modified glassy carbon electrode: pipette 1 μL of the dispersion B prepared in step b with a micro-syringe and drop-coat it on the surface of the glassy carbon electrode treated in step a, and dry it at room temperature. The solvent was volati...

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Abstract

The invention relates to a method for measuring dopamine by utilizing graphite nano-sheet / Nafion composite film to modify an electrode, belonging to the technical field of electrochemical analysis. The invention adopts cation polymer Nafion as a dispersing agent, disperses graphite nano-sheet with stable property into Nafion solution, coats the dispersing solution on the surface of a glassy carbon electrode in a dripping mode to prepare a chemical modifying electrode, uses a differential pulse voltammetry for phosphate buffer solution containing ascorbic acid, and has good selectivity and high sensitivity to the measurement of dopamine. The method can directly be used for fast electromagnetic measurement of the dopamine and has the advantages of high sensitivity, accuracy and selectivity and the like.

Description

technical field [0001] The invention belongs to the technical field of electrochemical analysis and detection, in particular to a method for measuring dopamine by using a graphite nanosheet / Nafion composite thin film modified electrode. It is suitable for the determination of dopamine in the presence of ascorbic acid interference. Background technique [0002] As a very important neurotransmitter, dopamine has an important effect on the coordination of pituitary endocrine function and the activity of nervous system. The imbalance of dopamine can lead to schizophrenia, Parkinson's disease, bronchial asthma and other diseases. Therefore, the study of its determination is of great significance to explore the physiological mechanism and the diagnosis of related diseases. At present, the methods for the determination of dopamine mainly include: chromatography, fluorescence spectrometry, electrochemical method, etc. Because the electrochemical analysis method has the advantages...

Claims

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Application Information

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IPC IPC(8): G01N27/48G01N27/30
Inventor 陈旭陈思明杨文胜
Owner BEIJING UNIV OF CHEM TECH
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