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Glabrous sarcandra herb extract, quality control method for extract preparation and application of thereof

A quality control method and extract technology, applied in anti-inflammatory agents, pill delivery, suppository delivery, etc., can solve the problems of inability to comprehensively evaluate the quality of the extract of Chinese herbal medicine Scutellaria spondylolis, achieve excellent precision, simple operation method, Highly stable effect

Inactive Publication Date: 2009-12-30
JINAN UNIVERSITY
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Problems solved by technology

) Since there is no relevant literature report on anti-inflammatory activity of isoxapyridine, it is only used as an index component, and it is impossible to fully evaluate the quality of the Chinese herbal medicine Scutellaria sargassum extract

Method used

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  • Glabrous sarcandra herb extract, quality control method for extract preparation and application of thereof
  • Glabrous sarcandra herb extract, quality control method for extract preparation and application of thereof
  • Glabrous sarcandra herb extract, quality control method for extract preparation and application of thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] The establishment of the standard fingerprint collection of embodiment 1 Sargassum extract:

[0049] Take 500g of the dried aboveground part of the traditional Chinese medicine Rhizoma sargassum, reflux twice with 10 times the amount of water, two hours each time, combine the extracts, recover the solvent under reduced pressure, freeze-dry after concentration, and obtain 51.2g of Rhizoma sargassum extract.

[0050] Dissolve the above-mentioned standard extract of sargassum with appropriate amount of water to make a solution containing 5.0 mg of sargassum extract per 1 mL of water. After passing through a 0.45 μm filter membrane, the test solution of the sargassum extract is obtained.

[0051] Take the reference substance of rosmarinic acid, dissolve it with an appropriate amount of methanol, and make a solution containing 1 mg of rosmarinic acid per 1 mL of methanol, and obtain the reference substance solution of rosmarinic acid.

[0052] Accurately take 10 μL of the ab...

Embodiment 2

[0053] Embodiment 2 Standard Fingerprint Spectrum Reproducibility Investigation:

[0054] Accurately weighed 25 mg of Sargassum standard extract in total 6 parts, respectively dissolved in water and fixed volume in a 5mL volumetric flask, after passing through a 0.45 μm filter membrane, took the filtrate as the test solution of Sargassum extract, using Example 1 Carry out chromatographic analysis under the liquid phase condition described in, record chromatogram (see image 3 ). In the HPLC fingerprint spectrum that obtains, the relative retention time of twelve chromatographic peaks all meets the relevant requirement in embodiment 1, and the relative deviation of the relative retention time of each chromatographic peak is all less than 1.0% (seeing table 1), shows that embodiment 1 The obtained HPLC fingerprints have good reproducibility. Utilize the " Chinese medicine chromatographic fingerprint similarity evaluation system 2004A " software calculation provided by Pharmaco...

Embodiment 3

[0057] Enrichment and structural identification of embodiment 3 chromatographic peaks:

[0058] Take 4kg of the dried aboveground part of the traditional Chinese medicine Zygomyceps sarcophagus, reflux twice with 10 times the amount of water, each time for two hours, combine the extracts, recover the solvent under reduced pressure, freeze-dry after concentration, and obtain 460 g of ZJF extract (ZJF). Suspend it in appropriate amount of water, adopt macroporous resin Diaion Hp-20 open column chromatography, and elute with water, 30% ethanol-water, 50% ethanol-water and 95% ethanol-water respectively. The solvent was recovered under reduced pressure respectively to obtain 340 g of water eluate (ZJF-I), 70 g of 30% ethanol-water eluate (ZJF-II), 50% chromatogram, and methanol-water (10, 30, 50, 100% ) gradient elution. Compounds I, II, and III were obtained from the fraction eluted with 10% methanol under the ODS column (ZJF-IA) by reverse-phase high-efficiency liquid phase sep...

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Abstract

The invention relates to a glabrous sarcandra herb extract and a quality control method for an extract preparation. The quality control method comprises the steps of establishing a fingerprint by adopting a reversed phase high performance liquid chromatograph and confirming a standard fingerprint; the standard fingerprint comprises twelve chromatographic peaks, wherein the chemical structures of ten chromatographic peaks are confirmed; the similarity between the fingerprint of a test product of the extract and related preparation and the standard fingerprint is not less than 0.65; in addition, a content measurement method for a plurality of anti-inflammatory active components is established by a working curve method, can effectively control the product quality of a Chinese medicament of the glabrous sarcandra herb extract and the related preparation, and has high reproducibility, high stability, and excellent precision; and the operation method is simple and convenient.

Description

technical field [0001] The invention relates to a chemical analysis method of a traditional Chinese medicine extract and related medicines, in particular to a quality control method and application of the traditional Chinese medicine sargassum extract and its preparation. Background technique [0002] The traditional Chinese medicine Sarcandra glabra (Thunb.) Nakai is the whole plant of Sarcandra glabra (Thunb.) Nakai, also known as Sarcandra glabra (Thunb.) Nakai, etc. It is a commonly used Chinese herbal medicine in my country and is used to treat blood heat purpura, purpura, rheumatic arthralgia, bruises and other diseases. Single preparations made from the water of Zhongjiefeng or ethanol extracts of different concentrations, such as: Zhongjiefeng Tablets, Zhongjiefeng Injection, Qingrexiaoyanning Tablets, Qingrexiaoyanning Capsules, or compounded with other traditional Chinese medicine extracts Preparations, such as: compound grass coral lozenges, Xinhuang tablets, Want...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/185A61K9/20A61K9/14A61K9/48A61K9/02A61K9/10A61K9/08A61K9/12A61K9/06A61K9/107G01N30/02A61P29/00A61P7/04A61P19/00
Inventor 戴毅姚志红赵烽段营辉潘明宇姚新生
Owner JINAN UNIVERSITY
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