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Duck virus hepatitis inactivated vaccine and preparation method thereof

A technology for duck viral hepatitis and inactivated vaccine, which is used in antiviral agents, pharmaceutical formulations, medical preparations containing active ingredients, etc.

Active Publication Date: 2009-12-30
QILU ANIMAL HEALTH PROD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there is no commercial duck viral hepatitis vaccine in my country

Method used

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  • Duck virus hepatitis inactivated vaccine and preparation method thereof
  • Duck virus hepatitis inactivated vaccine and preparation method thereof
  • Duck virus hepatitis inactivated vaccine and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Purity Test of DHV QL Strain Virus Seed Virus

[0058] 1) No colonies were observed in the sterility test T.G, G.P tubules and G.A slant medium.

[0059] 2) Mycoplasma test During the observation period, no obvious changes in the culture color of vials and small tubes were found, and the transplanted liquid cultures had no "fried egg" mycoplasma colonies on the solid medium.

[0060] 3) Exogenous virus test All the chicken embryos in the two groups survived within 7 days, the fetal development was normal, the chorioallantoic membrane had no lesions, and the chicken embryo fluid had no hemagglutination.

[0061] No bacteria, mold and mycoplasma were found in the DHV QL strain virus, and there was no exogenous virus contamination, which proved that it was pure.

Embodiment 2

[0063] vaccine preparation

[0064] 1 poison seed breeding

[0065] Dilute the virus seeds with sterile saline to 10 -3 , inoculate 9-10-day-old SPF chicken embryos in the allantoic cavity, 0.2ml per embryo, 96 hours after inoculation, cool at 0-4°C for 4-24 hours, harvest the embryo fluid, select no bacterial growth, virus content ≥ 10 6.0 EID 50 The embryo fluid of / 0.2ml is used as the poison seed for production.

[0066] 2 Preparation of venom for seedling production

[0067] Well-developed, 9-10-day-old susceptible chicken embryos were selected as materials for making seedlings. Get the seed poison for production, make 10 with sterilized physiological saline -2 Dilute, inoculate 9-10 day-old non-immune chicken embryos into the allantoic cavity, 0.2ml per embryo, seal the pinhole after inoculation, and continue incubation at 36-37°C.

[0068] 3 Incubation and observation

[0069] Eggs were illuminated once a day after inoculation of chicken embryos, and chicken embr...

Embodiment 3

[0081] Finished product inspection of vaccines

[0082] (1) Physical properties

[0083] Appearance should be a milky white emulsion.

[0084] The dosage form is water-in-oil (W / O). Take a clean straw, suck a small amount of vaccine and drop it in cold water, it should be in the shape of an oil drop without spreading.

[0085] Viscosity Use a 1ml straw (the inner diameter of the outlet is 1.2mm), absorb the vaccine at about 25°C, let it flow out vertically and naturally, and record the time required for the outflow of 0.4ml, which should not exceed 8 seconds.

[0086] Stability Store at around 37°C for 21 days or add 5ml of the vaccine into a centrifuge tube and centrifuge at 3000r / min for 15 minutes, the emulsion should not break.

[0087] Those who do not meet the requirements shall be judged as unqualified.

[0088] (2) The sterility test shall be carried out according to the provisions of the "Chinese Veterinary Pharmacopoeia", and the growth shall be sterile.

[0089...

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Abstract

The invention relates to duck virus hepatitis inactivated vaccine and a preparation method thereof. The preparation method comprises the following steps: taking a duck hepatitis virus virulent strain CGMCC No.3202 with excellent immunogenicity and obtained by the inventor through field separation as a viral strain for producing vaccine; inoculating the viral strain in a susceptible chick embryo and obtaining the embryo fluid; and carrying out emulsification after inactivation to prepare the safe effective duck virus hepatitis vaccine. The duck virus hepatitis inactivated vaccine aims to prevent duck virus hepatitis virus infection and fills a gap in the market, thereby providing conditions for controlling the spread of duck virus hepatitis on poultry farms.

Description

technical field [0001] The invention relates to an inactivated duck viral hepatitis vaccine and a preparation method thereof, belonging to the technical field of veterinary biological products. Background technique [0002] Duck Viral Hepatitis (DVH) is a highly lethal viral disease of ducklings caused by Duck Viral Hepatitis Virus (DHV). It is characterized by a short course of disease, clinical manifestations of opisthotonus, and the main lesion is hepatomegaly with bleeding spots. DVH not only causes morbidity and high mortality in ducklings, but also causes recessive infection in adult ducks, sheds toxins outward, and becomes a disseminator of pathogens. When the disease breaks out, ducklings die at an alarming rate. If the disease is not controlled in time, it will bring great economic losses to the duck industry. [0003] The viral particles of duck hepatitis virus are non-enveloped and belong to the Picornaviridae family. Virus particles are icosahedral symmetry, w...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/29A61P31/14
Inventor 徐龙涛李秀娟王蕾禚宝山
Owner QILU ANIMAL HEALTH PROD
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