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Method for preparing arachidonic acid (ARA) by recycling fungal meal of Mortierella alpine

A technology of arachidonic acid and Mortierella alpina, which is applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as repeated utilization, avoid emissions, increase production, and facilitate crushing effect

Active Publication Date: 2009-12-30
CABIO BIOTECH WUHAN CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

None of the existing published documents mentions the reuse of waste bacterial meal in the production of arachidonic acid

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1 Mortierella alpina meal recycle application pre-treatment:

[0034] 1.1. Mortierella alpina meal compound enzyme process treatment. Mortierella alpina meal is crushed, passed through a 60-mesh sieve, added with water to prepare a turbid liquid with a concentration of 20% by mass, the pH is kept natural, the temperature is raised to 50°C, and 0.5-3% of the mass percentage of Mortierella alpina meal is added For neutral protease, keep warm at 50°C and stir intermittently for 1 to 2 hours by stirring for 2 minutes every 10 minutes. For the prepared Mortierella alpina protease hydrolyzate, the existing technology is used to add the Mortierella alpina protease hydrolyzate as a nitrogen source to the fermentation medium, and after being sterilized with other raw materials, it is used for the fermentation and cultivation of Mortierella alpina.

[0035] 1.2. Mortierella alpina meal compound enzyme process treatment. Mortierella alpina meal is crushed, passed thro...

Embodiment 2

[0038] Optimization of Fermentation Process of Mortierella alpina

[0039] Mortierella alpina meal is crushed, passed through a 60-mesh sieve, and a certain amount of water is added to prepare a turbid solution with a concentration of 20% by mass. The pH is kept natural. The temperature is raised to 50°C, and 0.5-3% of the mass of Mortierella alpina meal is added. Percent neutral protease, keep warm at 50°C, and stir intermittently for 1 to 2 hours by stirring for 2 minutes every 10 minutes. The prepared Mortierella alpina protease hydrolyzate is added to the fermentation medium as a nitrogen source, and is used for fermentation and cultivation of Mortierella alpina after being sterilized with other raw materials. The nitrogen source in the shake flask medium was replaced at different replacement ratios.

[0040] The Mortierella alpina seed shake flask growth medium adopts the original growth medium formula, and the prepared growth medium is kept at 121°C for 30 minutes. Ino...

Embodiment 3

[0046] Optimization of Fermentation Process of Mortierella alpina

[0047] Mortierella alpina meal is crushed, passed through a 80-mesh sieve, and a certain amount of water is added to prepare a turbid liquid with a concentration of 20% by mass, the pH is adjusted to 8.0-9.0, the temperature is raised to 50°C-55°C, and Mortierella alpina is added The mass percentage of the meal is 0.5-3% of neutral protease, the temperature is kept at 50°C, and the mixture is stirred intermittently for 1-3 hours in a manner of stirring for 2 minutes every 10 minutes. The prepared Mortierella alpina protease hydrolyzate is added to the fermentation medium as a nitrogen source, and is used for fermentation and cultivation of Mortierella alpina after being sterilized with other raw materials. The nitrogen source in the shake flask medium was replaced at different replacement ratios.

[0048] The Mortierella alpina seed shake flask growth medium adopts a formula in which 50% of the nitrogen sourc...

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Abstract

The invention relates to a method for preparing arachidonic acid (ARA) by utilizing Mortierella alpine mycelia, comprising the following steps: first carrying out deep culture on the inoculation amount of the Mortierella alpine in a constant temperature shaker; transferring and inoculating the Mortierella alpine into a container with fermentation media, obtaining fermented liquor after culturing; filtering the fermented liquor, collecting mould organisms, washing, drying and grinding the collected mould organisms, extracting or pressing the mould organisms with a nonpolar solvent, harvesting oil and fat and further processing the harvested oil and fat to obtain the target product, wherein, parts of commonly used nitrogen sources of growth media or / and fermentation media are substituted by fungal meal of Mortierella alpine and the substitution ratio of the nitrogen sources in the fungal meal of Mortierella alpine is 10-90 by mass. The residual solid matter after extracting the mould organisms with the nonpolar solvent is the recyclable fungal meal of Mortierella alpine. The wastes generated in the process of producing the ARA by utilizing the Mortierella alpine by fermentation can be recycled, thus avoiding discharge of the environmental pollutants.

Description

technical field [0001] The invention relates to a production method of microbial fermentation, in particular to a method for preparing arachidonic acid from Mortierella alpina mycelium. Background technique [0002] In recent years, the physiological and therapeutic functions of long-chain polyunsaturated fatty acids in the human body have received more and more attention and recognition. There are already a large amount of information and literature introducing the production of bioengineering methods, and many products have already entered the market. The fermentation of arachidonic acid is mainly produced by Mortierella alpina, which is fermented by Mortierella alpina to obtain mold organisms, and the enriched mold organisms are filtered, dried, and extracted to obtain arachidonic acid (ARA). Microbial residue, this microbial residue is called Mortierella alpina meal (soybean meal after extracting oil with soybean). From the current relevant information, it can be seen ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64C12R1/645
Inventor 汪志明唐孝鹏张玉良王纪尚耘马凡提易华荣耿安峰肖子豪肖敏
Owner CABIO BIOTECH WUHAN CO LTD
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