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Polypeptide with activity of promoting fibroblast growth factor receptor 3 and its screening method and application

A technology of growth factor receptors and fibroblasts, applied in the field of peptides, can solve the problems of no FGFs and other problems, and achieve the effects of quality control, low immunogenicity, and small molecular weight

Inactive Publication Date: 2011-12-07
INST OF FIELD OPERATION SURGERY NO 3 MILITARY MEDICL UNIV PLA
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although 23 FGFs have been discovered so far, no FGFs that only specifically bind to FGFR3 have been found.

Method used

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  • Polypeptide with activity of promoting fibroblast growth factor receptor 3 and its screening method and application
  • Polypeptide with activity of promoting fibroblast growth factor receptor 3 and its screening method and application
  • Polypeptide with activity of promoting fibroblast growth factor receptor 3 and its screening method and application

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Embodiment Construction

[0028] The invention adopts the phage display technology to screen the polypeptide with the activity of promoting FGFR3. Phage display technology is to insert the DNA sequence of foreign protein or polypeptide into the appropriate position of the phage coat protein structural gene, so that the foreign gene is expressed with the expression of the coat protein, and at the same time, the foreign protein or polypeptide is displayed with the reassembly of the phage Biotechnology to the surface of phages. The foreign protein or polypeptide expressed on the surface of phage can specifically bind to the target molecule. After the target molecule is coated on the solid phase carrier, the phage display peptide library is screened, and the phage expressing the corresponding foreign protein or polypeptide can be isolated and purified from many phages, and the amino acid sequence of the foreign protein or polypeptide can be Obtained by DNA sequencing of phages.

[0029] The screening of ...

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Abstract

The invention discloses a polypeptide having the activity of promoting fibroblast growth factor receptor 3 (FGFR3) and its screening method and application. The polypeptide consists of Val-Ser-Pro-Pro-Leu-Thr-Leu-Gly-Gln-Leu -The amino acid sequence composition shown by Leu-Ser; the screening method is to co-incubate the phage 12 peptide library with the microtiter plate coated with FGFR3, wash away the non-specific binding phage, and then elute the specific binding phage and amplify As the input phage for the next round of panning, repeat the above steps for a total of three rounds of panning, extract the DNA of the single clone of the phage selected in the third round for sequencing, and deduce the amino acid sequence of the exogenous polypeptide displayed on the phage , synthesize the polypeptide, and detect its promoting effect on the activity of FGFR3, so as to obtain the polypeptide having the ability to promote the activity of FGFR3; the polypeptide can be used to prepare drugs for diseases with low FGFR3 function.

Description

technical field [0001] The invention relates to a polypeptide, in particular to a polypeptide having the activity of promoting fibroblast growth factor receptor 3, and also relates to a screening method and application of the polypeptide. Background technique [0002] Fibroblast growth factor receptors (FGFRs) are a class of transmembrane tyrosine kinase receptors with autophosphorylation activity, and their corresponding ligands, fibroblast growth factors (FGFs) It plays an important role in the process of tissue and organ development and injury repair. [0003] At present, four kinds of FGFRs have been found, namely FGFR1, FGFR2, FGFR3 and FGFR4. Among them, FGFR3 is a negative regulatory molecule that mediates endochondral ossification and plays an important role in bone development. Previous studies have shown that FGFR3 gain-of-function gene mutations inhibit the proliferation and differentiation of chondrocytes in the epiphyseal growth plate, causing a variety of ske...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/566A61P19/08C07K7/08A61P19/02C07K1/22A61P19/10A61K38/10
Inventor 陈林余瑛黄炜杜晓兰金旻戚华兵苏楠赵玲谢杨丽杨京何启芬
Owner INST OF FIELD OPERATION SURGERY NO 3 MILITARY MEDICL UNIV PLA
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