A method for preparing materials having antithrombotic activity from muskrat musk and materials obtained from the method

An anti-thrombotic and muskrat technology, applied in the field of high-efficiency anti-thrombotic agents, anti-thrombotic agents, and the preparation of anti-thrombotic agents, to achieve the effect of increasing added value

Inactive Publication Date: 2010-02-03
PUSAN NAT UNIV IND UNIV COOPERATION FOUND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] However, there are no studies on the isolation of substances with antithrombotic activity from muskrat incense

Method used

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  • A method for preparing materials having antithrombotic activity from muskrat musk and materials obtained from the method
  • A method for preparing materials having antithrombotic activity from muskrat musk and materials obtained from the method
  • A method for preparing materials having antithrombotic activity from muskrat musk and materials obtained from the method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0072] Embodiment 1: the ethanol extract of muskrat incense

[0073] see figure 1 , muskrat incense is continuously extracted with an organic solvent, and the muskrat incense is collected from muskrats and stored in a cool room.

[0074] Specifically, 40 g of muskrat was added to an Erlenmeyer flask, ethanol (5 times the weight of muskrat, 200 ml) was added thereto, and the mixture was shaken at 150 rpm for 24 hours and centrifuged at 20,000 rpm to obtain a supernatant.

[0075] An equal amount of ethanol was added to the residue to repeat the ethanol extraction to obtain another ethanol extract other than the above ethanol extract.

[0076] Add 200ml of ethyl acetate to the residue, and collect the ethyl acetate extract according to the method shown in ethanol extraction. 200 ml of hexane was further added to the residue, and the hexane extract was collected according to the method shown in ethanol extraction.

Embodiment 2

[0078] Example 2: The 1st normal phase column chromatography and thin layer chromatography of ethanol extract of muskrat incense

[0079] see figure 2 , the ethanol extract obtained in Example 1 was subdivided through the first normal phase column chromatography.

[0080] The column filler was normal phase silica gel (230 to 400 mesh), and the solvent as the mobile phase was hexane and ethyl acetate, wherein the combined ratio of the solvents was increased from 400:1 to 1:1, and 116 fractions were obtained.

[0081] Thin-layer chromatography was used to identify the material spectrum of each fraction, and the developing solvent used was hexane and ether (10:1.5).

[0082] The spectrum of each fraction was further identified by short-wave UV lamp (254nm) and long-wave UV lamp (365nm). After treating the thin-layer chromatography plate with 10% sulfuric acid and heating, the spectrum was again identified by the colored spots.

[0083] According to the above identification ...

Embodiment 3

[0090] Example 3: The second normal phase column chromatography and thin layer chromatography of the first fraction

[0091] see figure 2 , in Example 2, the B1-5 group showed higher antithrombotic activity than aspirin, and this group was subdivided by the second normal phase column chromatography.

[0092] The solvent used as the mobile phase was hexane and ethyl acetate, wherein the combined ratio of the solvents was increased from 70:1 to 1:1, and 172 fractions were obtained.

[0093] According to the method shown in Example 2, identify the collection of collections of each fraction obtained above, and the results are listed in Figure 5 middle. Each fraction was divided into 9 groups (B2-1 to B2-9) according to the moving distance, and used for measurement of antithrombotic activity.

[0094] Measure the antithrombotic activity of the material obtained by the normal phase column for the second time according to the method in Example 2, and as Image 6 As shown, in ...

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Abstract

Disclosed herein is a method of preparing an antithrombotic agent from muskrat musk and an antithrombotic agent having a high potency obtained therefrom, wherein the antithrombotic agent is prepared by treating muskrat musk with ethanol to obtain an ethanol extract; carrying out two normal phase column chromatographies while raising the combination ratio of hexane and ethyl acetate to obtain numerous fractions; carrying out thin layer chromatographies and identifying the material patterns of the fractions with UV lamps and 10% sulfuric acid to divide the materials which have a similar moving distance into groups; measuring a thrombin time of each group to isolate a group having a potent antithrombotic activity; carrying out a reverse phase column chromatography while raising the combination ratio of acetonitrile and water to obtain numerous fractions; and isolating an antithrombotic agent material having a high potency therefrom. According to the present invention which provides an antithrombotic agent having a high potency from muskrat musk, the value added of muskrat musk is raised.

Description

technical field [0001] The invention relates to a method for preparing an antithrombotic agent from muskrat incense, and an antithrombotic agent with high efficacy obtained by the method. More specifically, the present invention relates to a method for preparing an antithrombotic agent from muskrat incense, and an antithrombotic agent with high efficacy obtained by the method, the method comprising: treating muskrat incense with an organic solvent to obtain an organic The solvent extract was subjected to two normal phase column chromatography and one reverse phase column chromatography to obtain multiple fractions, and the thrombin time of each fraction was measured. Background technique [0002] Muskrat (Ondatra zibethicus) is a mammal belonging to the family Rodentidae, oval in shape, with a body length of about 35 cm, a tail length of 25 cm, and a weight of about 1 kg. Muskrats are often near weed-filled swamps and lakes. Muskrats are active from spring to late autumn, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/48A61K35/12A61K9/20A61P7/02
CPCA61K35/48A61K35/12A61P7/02
Inventor 金根基姜韩锡崔勇株申泽顺金研玖李宰浩金勇均全尚珢
Owner PUSAN NAT UNIV IND UNIV COOPERATION FOUND
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