Method for producing xylitol and L-arabinose by xylose mother liquor

A technology for mother liquor of arabinose and xylose, applied in the direction of microorganism-based methods, biochemical equipment and methods, chemical instruments and methods, etc., can solve the problem of poor utilization of mother liquor of xylose, reduction of xylose benefits, and waste of resources Environmental and other issues, to achieve the effect of reducing content, improving purity, and reducing environmental pollution

Active Publication Date: 2010-02-10
安徽丰原化工装备有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the xylose mother liquor has not been well utilized. It is generally sold to caramel pigment factories at low prices to produce caramel pigment, which greatly reduces the benefits of xylose and xylitol production, and at the same time causes a lot of waste of resources and serious environmental problems. Pollution

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A kind of method utilizing xylose mother liquor to produce xylitol, L-arabinose, comprises the steps:

[0023] (1) Xylose mother liquor fermentation xylitol

[0024] The waste xylose mother liquor obtained after twice crystallization of corn cob hydrolyzate to extract xylose crystals, the sugar concentration in this xylose mother liquor is about 70% to 80%, of which glucose is 5 to 15%, and xylose is 40 to 50%. Arabinose 15-20%.

[0025] Dilute the xylose mother liquor with water to a xylose concentration of 15% (mass volume percentage), add in the fermenter, add 0.1% (mass volume percentage) of ammonium sulfate and 0.3% (mass volume percentage) of corn steep liquor, and heat to 121 ° C , maintained for 25 minutes, cooled to 32°C, inserted into the cultured Candida tropicalis seed liquid, the inoculation amount was 10% (volume percentage) of the fermentation liquid volume, controlled fermentation pH6.0, fermentation temperature 30-32°C, ventilated Ratio (volume of liq...

Embodiment 2

[0032] A kind of method utilizing xylose mother liquor to produce xylitol, L-arabinose, comprises the steps:

[0033] (1) Xylose mother liquor fermentation xylitol

[0034] The xylose mother liquor is the same as in Example 1, diluted with water to a xylose concentration of 25%, added to a fermenter, added 0.1% ammonium sulfate and 0.3% corn steep liquor, heated to 121°C, maintained for 25 minutes, cooled to 32°C, Insert the cultivated Candida tropicalis seed solution, the inoculum amount is 10% of the volume of the fermentation solution, control the fermentation pH to 6.0, the fermentation temperature is 30-32°C, the ventilation ratio is 1:0.2, and after 75 hours of fermentation, xylitol is obtained Concentration is 16.5% fermented liquid;

[0035] (2) Filtrating the fermentation broth through a ceramic membrane with a molecular weight cut-off of 300KD to remove bacteria and some macromolecular substances to obtain a fermentation serum;

[0036] (3) The fermentation superna...

Embodiment 3

[0041] A kind of method utilizing xylose mother liquor to produce xylitol, L-arabinose, comprises the steps:

[0042] (1) Xylose mother liquor fermentation xylitol

[0043] The xylose mother liquor is the same as in Example 1, diluted with water to a xylose concentration of 10%, added to a fermenter, added 0.1% ammonium sulfate and 0.3% corn steep liquor, heated to 121°C, maintained for 25 minutes, cooled to 32°C, Insert the cultured Candida tropicalis seed solution (you can also use bacteria, actinomycetes or molds that can utilize xylose and glucose and produce xylitol as seeds, and the fermentation conditions are properly adjusted according to conventional techniques), and the inoculum size is 10% of the volume of the fermentation broth, controlled fermentation pH6.0, fermentation temperature 30-32°C, ventilation ratio 1:0.2, after 62 hours of fermentation, a fermentation broth with a xylitol concentration of 7.5% was obtained;

[0044] (2) Sedimentation and separation of ...

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Abstract

The invention relates to a method for producing xylitol and L-arabinose by xylose mother liquor. The method comprises the following steps: producing strains by the xylitol to ferment the xylose motherliquor, and eliminating thalli, pigment and impurities from fermentation liquor followed by condensing and crystallizing to obtain a xylitol crystal and the xylitol mother liquor, wherein, the xylitol mother liquor is separated by a chromatographic system to obtain an L-arabinose phase, and then the L-arabinose phase condensed and crystallized to obtain an L-arabinose phase crystal. The method ofthe invention helps fully utilize the industrial waste xylose mother liquor as a raw material to produce the xylitol and the L-arabinose at the same time, wherein, xylose and glucose in the xylose mother liquor are firstly utilized to ferment and synthesize the xylitol, thus increasing xylitol yield and reducing difficulty in separating the L-arabinose from the mother liquor. The method of the invention has wide raw material source, low price, less resource consumption, low production cost and reduced environmental pollution caused by industrial wastes.

Description

technical field [0001] The invention relates to a production process of xylitol and L-arabinose, in particular to a method for producing xylitol and L-arabinose by using waste xylose mother liquor in the production of xylose and xylitol. Background technique [0002] At present, a large amount of xylose mother liquor will be produced in the process of xylose production and hydrogenation xylitol production. Xylose mother liquor refers to the remaining mother liquor after xylose hydrolyzate is crystallized and xylose is extracted. Taking corn cob raw material as an example, most corn cob hydrolyzate contains 14% sugar mass concentration, which contains 13% glucose, 70% xylose, 15% arabinose and a small amount of other miscellaneous sugars, and xylose obtains crystals through twice crystallization, thus Hydrogenation is used to produce xylitol. The sugar concentration in the remaining xylose mother liquor is about 70%-80%, wherein glucose is 6-12%, xylose is 60-70%, and arabin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/18C07H3/02C07H1/06C12R1/74
Inventor 李荣杰尚海涛郑辉潘声龙
Owner 安徽丰原化工装备有限公司
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