Method for preparing almond peptide from almond dregs
A technology for almond meal and almonds, which is applied in the preparation methods of peptides, chemical instruments and methods, peptides, etc., can solve problems such as waste of nutrient resources, and achieve the effects of increasing added value and inhibiting effect.
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[0014] Example one:
[0015] (1) The dried almond meal is crushed with a pulverizer and passed through a 40-mesh sieve.
[0016] (2) Add deionized water to the almond meal powder, by mass ratio, the solid-liquid ratio is 1:20, the pH is adjusted to 7.5 with NaOH, the temperature is 40°C, and the mixture is stirred and leached under these conditions for 1 hour. Centrifuge at 3000r / min for 10min, and take the supernatant. The supernatant was adjusted to pH 4.5 with HCL, centrifuged at 3000 r / min for 30 min, and the precipitation layer was washed with water once. Collect the precipitate.
[0017] (3) Put the precipitate obtained above in the enzymatic hydrolysis tank, add deionized water according to the mass ratio of crude almond protein: deionized water of 1:10, stir and mix, heat to 40°C, adjust the pH to 5 with HCL, The mass ratio of protease:crude almond protein is 1:100, and papain is added for enzymatic hydrolysis for 2 hours.
[0018] (4) Adjust the pH to 5.5 and quickly raise...
Example Embodiment
[0023] Embodiment two:
[0024] (1) The almond meal obtained by squeezing the almond oil is dried and then pulverized with a pulverizer and passed through a 60-mesh sieve.
[0025] (2) Add deionized water to the almond meal powder, adjust the pH to 9.5 with NaOH and the temperature at 55°C according to the mass ratio of solid-liquid ratio of 1:25, stir and extract under these conditions for 3 hours, at 6000r Centrifuge for 30 min at / min and take the supernatant. The supernatant was adjusted to pH 4.5 with HCL, centrifuged at 6000 r / min for 30 min, and the precipitation layer was washed with water 3 times. Collect the precipitate.
[0026] (3) Put the above-obtained precipitate in the enzymatic hydrolysis tank, add deionized water according to the mass ratio of crude almond protein: deionized water of 1:15, stir and mix, heat to 55°C, adjust the pH to 8, according to the protease: The mass ratio of crude almond protein was 2:100, and neutral protease was added to carry out enzymat...
Example Embodiment
[0031] Embodiment three:
[0032] (1) The almond meal obtained by squeezing the almond oil is dried and then pulverized with a pulverizer and passed through a 50 mesh sieve.
[0033] (2) Add deionized water to the almond meal powder, and adjust the pH to 8.0 with NaOH and the temperature at 50°C according to the mass ratio of solid-liquid ratio of 1:23. Under these conditions, stir and extract for 2 hours at 5000r Centrifuge for 20 min / min, and take the supernatant. The supernatant was adjusted to pH 4.5 with HCL, centrifuged at 4500 r / min for 20 min, and the sediment layer was washed twice with water. Collect the precipitate.
[0034] (3) Put the above-obtained precipitate in an enzyme hydrolysis tank, stir and mix according to the mass ratio of crude almond protein: deionized water of 1:13, heat to 50°C, adjust the pH value to 7, according to the protease: crude almond protein as 1.5 The mass ratio of :100 was added with neutral protease to carry out enzymatic hydrolysis for 4 ...
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