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Isopentenyl flavone and application thereof

A technology of prenyl flavonoids and diprenyl quercetin, which is applied in the field of preparation of drugs for the treatment of breast cancer, and can solve problems such as the lack of breast cancer reports of basilica and its compounds

Inactive Publication Date: 2010-02-17
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] So far, there has been no report on the inhibition of breast cancer by lobular lotus and its compounds

Method used

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  • Isopentenyl flavone and application thereof
  • Isopentenyl flavone and application thereof
  • Isopentenyl flavone and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Dried lobular lotus medicinal material, about 18kg after crushing, reflux extraction twice with 8 times the amount of 95% ethanol, the extraction time is 2 hours and 1 hour respectively, recover the ethanol, concentrate to 70 ℃ and the extract is about 5.2kg with a relative density of 1.10 . Take about 4.8kg, add 10L of distilled water and stir until completely suspended, then extract 5 times with petroleum ether, ethyl acetate, and water-saturated n-butanol in equal volume. The solvent was recovered from the extract under reduced pressure to obtain various extraction fractions. Get 274g of ethyl acetate part, separate through silica gel column (100~200 mesh) chromatography, chloroform-methanol (10:0~0:10) gradient elution, according to TLC test result, in chloroform:methanol is 40:1~ The 30:1 polar site contains 8,2′-diprenylquercetin 3-methyl ether, purified by Sephadex LH-20, eluted with chloroform-methanol (6:4) to give 8,2′-di Prenyl quercetin 3-methyl ether 60mg...

Embodiment 2

[0018] The inhibitory effect of 8,2′-diprenyl quercetin 3-methyl ether on human breast cancer cells was detected by acid phosphatase method.

[0019] Human breast cancer MDA-231 cell line and human breast cancer T47D cell line were cultured in DMEM medium containing 10% newborn bovine serum, 100 U / mL penicillin, and 100 U / mL streptomycin at 37°C and 95% humidity , 5% CO 2 Incubator conditions; conventional 2.5g / L trypsin digestion and passage.

[0020] After digesting the cells in the logarithmic growth phase, make a cell suspension by 1.5×10 3 The density per well was inoculated in a 96-well culture plate. After the cells were completely adhered to the wall after 24 hours of culture, the experimental group was added with different concentrations of compounds; the blank control group was added with DMEM culture medium. In the experiment, set 6 parallel wells for each concentration, discard the medium in the 96-well plate after 24h, 48h, and 72h of action, wash each well with...

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Abstract

The invention relates to an isopentenyl flavone and an application of the compound to the preparation of a medicament for treating breast cancer. The isopentenyl flavone is a new compound of 8,2'-diprenylquercetin3- methyl etherseparated from fructus podophylli. The experiment of the inhibited effect on human breast cancer cells shows that the isopentenyl flavone has good inhibited effect on the human breast cancer cells.

Description

technical field [0001] The invention relates to a prenyl flavone and the application of the compound in the preparation of drugs for treating breast cancer. Background technique [0002] Fructus Sinopodophylli is a commonly used Tibetan medicine, and its source is recorded in the 2005 edition of the Chinese Pharmacopoeia as the dried ripe fruit of Podophyllum hexandrum Royle, a plant of the family Berberidaceae. It has the function of promoting blood circulation and regulating menstruation, and is often used to treat various gynecological diseases. However, there are few reports on the research on Lily lobata at present. [0003] At present, the main compounds isolated from the lotus are: [0004] Lignanoids compounds: podophyllotoxin, deoxypodophyllotoxin, 4'-nordeoxypodophyllotoxin (4'-demethyldesoxypodophyllotoxin). [0005] Flavones: quercetin, 8-prenylkaemferol and citrusinol. [0006] So far, there has been no report on the inhibition of breast cancer by lobular lo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/30A61K31/352A61P35/00
Inventor 尚明英孔越肖军军马祥君葛跃伟蔡少青
Owner PEKING UNIV
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