Novel process for producing sodium heparin

A technology of heparin sodium and a new process, which is applied in the field of new production process of extracting heparin sodium from pig small intestinal mucosa by using low-temperature method of biological enzymatic hydrolysis, which can solve the problems of large energy consumption, long production cycle, incomplete hydrolysis, etc., and achieve automation The effect of high degree of control, low production cost and low production energy consumption

Inactive Publication Date: 2010-02-17
SICHUAN TIANCHENG BIOCHEM TECH
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  • Summary
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AI Technical Summary

Problems solved by technology

At present, the methods for producing heparin sodium in China are mainly salt hydrolysis and acid hydrolysis. These two methods all use the pig small intestinal mucosa as raw material, and undergo salt hydrolysis and enzymatic hydrolysis under alkaline conditions. Adsorption and elution with strong alkaline cation exchange resin, and ethanol precipitation in the elu...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] ①Enzymolysis:

[0025] Inhale the fresh pig intestinal mucosa into the reaction pot, measure the volume, adjust the pH to 8.5-9.5 with NaOH, and the temperature is 45-50°C, add 3-5% NaCL and 0.03-0.036% fresh pancreas according to the volume of pig intestinal mucosa slurry, 0.03-0.036% trypsin, stir for 1-2 hours, keep warm for 2 hours, turn on the temperature to 70-80°C, stop heating, filter with filter cloth, and suck the filtrate into the adsorption tank;

[0026] ② Resin exchange adsorption:

[0027] Cool the filtrate below 50°C, measure the volume, adjust the pH to 8.5-9.0, add 8 liters of strong basic anion exchange resin per 1000 liters of filtrate, stir and absorb for more than 8 hours, collect the special resin for heparin sodium, and discard the waste liquid ; Determination of the content of heparin sodium in the discharged waste liquid requires less than 1.2;

[0028] ③Resin washing:

[0029] Wash with tap water and warm water first, and then wash heparin ...

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Abstract

The invention provides a novel process for producing sodium heparin, which comprises the following steps of enzymolysis, gum exchange adsorption, resin washing, heparin eluting, alcohol precipitation,precipitation once more, dehydration drying, crushing, mixing and packaging. The process has mature flow, convenient operation, no emission of waste intestinal dregs and overproof wastewater, complete enzymolysis reaction, short reaction time of 30 hours, low production energy consumption, low reaction temperature of between 70 and 80 DEG C, heparin extracting ratio over 90 percent, high unit yield of the heparin, only need of 1,700 pieces of small intestines of pigs for each 100 million units of heparin, low production cost, increase of economic benefit over 30 percent per 100 million units,no emission of dirty intestinal dregs and overproof wastewater, and suitability for large-scale production.

Description

technical field [0001] The invention relates to a production process of heparin sodium, in particular to a new production process for extracting heparin sodium from pig small intestine mucosa by using a biological enzymatic hydrolysis low temperature method. Background technique [0002] Heparin sodium is a natural polysaccharide extracted from biological materials. It is favored by patients and doctors in clinical use. It is widely used in the prevention and treatment of various cardiovascular and cerebrovascular diseases. It has always been the most effective anticoagulant drug, especially for Arterial and venous thrombosis and blood clots have a unique curative effect. Heparin sodium is widely distributed in the intestinal mucosa, lung, and liver of mammals, especially in the small intestinal mucosa of pigs. At present, the methods of domestic production of heparin sodium are mainly salt hydrolysis and acid hydrolysis. These two methods all use the pig small intestinal m...

Claims

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Application Information

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IPC IPC(8): C12P19/04C08B37/10
Inventor 蔡天贵刘泰山蔡静蔡成良胡小翠蔡承儒
Owner SICHUAN TIANCHENG BIOCHEM TECH
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