Method for producing mullein glucoside monomeric compound

A technology of verbascoside and compound is applied in the field of producing verbascoside monomer compound, which can solve problems such as differences in bioavailability and curative effect.

Inactive Publication Date: 2010-02-24
SUN YAT SEN UNIV +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In vivo pharmacological experiments have shown that echinacoside, the main component of phenylethanol glycosides, needs to be converted into verbascoside through the metabolic channel catalyzed by microbial enzymes in the large intestine during the movement process in the gastrointestinal tract. The main factors that have significant differences between utilization and efficacy

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 1. Select the fresh meaty stems of Cistanche deserticola that are kept fresh at 13°C in the industrial refrigerator, and use the usual industrial microwave heating device to inactivate the fresh fleshy stems of Cistanche deserticola at high temperature at 100°C for 4 minutes; In the grinding device, the fleshy stems of Cistanche are crushed and ground into Cistanche cell fragments, and the cell fragments of Cistanche are used as the substrate of the catalytic reaction, and β-glucosidase is used as the biocatalyst, and the solid-state fermentation reactor is used for the catalytic reaction, and the catalytic hydrolysis removes the substrate. A glucose group at the end of the echinacoside glucosyl ligand in the substance is directional converted into verbascoside monomer compound; the temperature of the catalytic reaction process is 46.5°C, the pH is 4.1, and the time is 5h, the β-glucose in the reactant during the catalytic reaction process The activity of sidase is 80U / g...

Embodiment 2

[0028] 1. Use the quick-frozen Cistanche succulent stems stored at -20°C in an industrial freezer, and use the usual industrial steam heating device to inactivate the fresh Cistanche succulent stems at high temperature. The temperature for the enzyme inactivation is 100°C for 10 minutes; In the crushing and grinding device, the meaty stems of Cistanche are crushed and ground into Cistanche cell fragments, and the cell fragments of Cistanche are used as the substrate of the catalytic reaction, and β-glucosidase is used as the biocatalyst, and a solid-state fermentation reactor is used for catalytic reaction and catalytic hydrolysis. Remove a glucose group at the end of the echinacoside glucosyl ligand in the substrate, and convert it into a monomer compound of verbascoside; the temperature of the catalytic reaction process is 48 ° C, the pH is 5.0, and the time is 6 hours. The β in the reactant during the catalytic reaction process - The activity of glucosidase is 2U / g; the conv...

Embodiment 3

[0032] 1. Select decoction pieces of Cistanche, and use the usual industrial microwave heating device to inactivate the fresh fleshy stems of Cistanche at high temperature. The temperature for inactivating the enzyme is 90°C for 5 minutes; then put it in the usual industrial crushing and grinding device for crushing and grinding to become Cistanche. The cell crushed product, the Cistanche cell crushed product is used as the substrate of the catalytic reaction, the water content of the catalytic reaction substrate is adjusted to 80%, the β-glucosidase is used as the biocatalyst, and the solid-state fermentation reactor is used for the catalytic reaction, and the substrate is removed by catalytic hydrolysis A glucose group at the end of the echinacoside glucosyl ligand is directional converted into verbascoside monomer compound; the temperature of the catalytic reaction process is 45 ° C, the pH is 5.8, and the time is 3 hours. The β-glucoside in the reactant during the catalytic ...

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Abstract

The invention relates to a method for producing mullein glucoside monomeric compound, which comprises the following steps: carrying out high-temperature enzyme deactivation and cell rupture on fleshystem fresh cistanche deserticola or cistanche deserticola decoction pieces; taking beta-glucosidase industrial enzyme preparation as biological catalyst; carrying out biocatalysis reaction by a solidstate fermentation reactor; rupturing glucose residue ligand terminal by echinacoside zymolyte in catalyzed hydrolysis substrate (belt-1,6-glycosidic bond); directionally translating into high-activity mullein glucoside monomeric compound; and then carrying out systematic process treatment, such as serosity extraction, separation and purification, concentration and drying and the like to obtain the mullein glucoside monomeric compound. By adjusting separation and purification process conditions, the purity percentage content of the meal or drying material of the obtained mullein glucoside monomeric compound can reach 35.8%-89.5%. The mullein glucoside monomeric compound has the characteristics of micromolecule and low polarity, can show good absorptivity and special pharmacology activity in drug absorption.

Description

technical field [0001] The invention relates to a method for producing verbascoside monomer compound through biocatalytic conversion by using fresh fleshy stems of Cistanche deserticola and decoction pieces of Cistanche deserticola. Background technique [0002] Cistanche deserticola is known as "ginseng" in the desert, and is a famous tonic traditional Chinese medicine. Analysis shows that the main active ingredients of Cistanche are phenylethanol glycosides, benzyl alcohol glycosides, iridoid glycosides, lignan glycosides, oligosaccharide derivatives, D-mannitol and betaine. Phenylethanoid Glycosides (Ph Gs) include active substances such as Echinacoside and Acteoside. In vivo pharmacological experiments have shown that echinacoside, the main component of phenylethanol glycosides, needs to be converted into verbascoside through the metabolic channel catalyzed by microbial enzymes in the large intestine during the movement process in the gastrointestinal tract. The main f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/44C07H15/18C07H1/00
Inventor 招淑燕刘昕彭青云田晓玲刘小卓钟欣雷桅李国富黄晓霓
Owner SUN YAT SEN UNIV
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