Unlock instant, AI-driven research and patent intelligence for your innovation.

Novel method for increasing yield of epsilon-poly-L-lysine

A kind of technology of lysine and new method, applied in the field of fermentation engineering

Active Publication Date: 2010-03-17
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
View PDF6 Cites 25 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] L-lysine is the direct precursor of ε-poly-L-lysine, but in the existing fermentation process of ε-poly-L-lysine, there is no report about adding L-lysine

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1 describes the existing production process;

Embodiment 2

[0022] Example 2, Example 3 and Example 4 are innovative processes compared with Example 1.

[0023] Example 1:

[0024] In a 5L fermentor equipped with 2.7L medium, inoculate 300mL amylase-producing Streptomyces chromogenes or Streptomyces albicans seed culture solution, cultivate at 30°C, and automatically control to 30% when the DO drops to 30%, and aerobic culture for 120h , The air flow rate is 4.0 ~ 6.0L / min. When the pH drops to about 6.0, add ammonia (25-30%) to maintain the pH at 6.0. When the residual sugar concentration in the fermentation broth drops to 10g / L, add glucose (400g / L) and (NH 4 ) 2 SO 4 (80g / L) Make the residual sugar concentration reach 13g / L. At the same time, the pH is no longer controlled, and it is naturally reduced to 4.0 and maintained at around 4.0.

[0025] After culturing for 120h, the fermentation was over. The highest accumulation of ε-poly-L-lysine in the fermentation broth was 10.7g / L. Centrifugation to remove the bacteria and part of the sol...

Embodiment 3

[0030] In a 30L fermentor equipped with 16.2L of medium, inoculate 1.8L of amylase-producing Streptomyces chromogenes or Streptomyces albicans secondary shake flask seed culture solution, cultivate at 30℃, and automatically control to 30 when the DO drops to 30% %, aerobic culture for 120h, the air flow rate is 4.0-6.0L / min. When the pH drops to about 6.0, add ammonia (25-30%) to maintain the pH at 6.0. When the residual sugar concentration in the fermentation broth drops to 10g / L, add glucose (400g / L) and (NH 4 ) 2 SO 4 (80g / L) Make the residual sugar concentration reach 13g / L, and add L-lysine (10g / L) to the fermentation broth. At the same time, the pH is no longer controlled, and it is naturally reduced to 4.0 and maintained at around 4.0.

[0031] After culturing for 120 hours, the fermentation was over. The highest accumulation of ε-poly-L-lysine in the fermentation broth was 14.6 g / L. Centrifugation to remove the bacteria and part of the solids, the supernatant was exchang...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a novel method for increasing the yield of epsilon-poly-L-lysine, comprising the following steps: adopting Streptomyces diastatochromogenes or Streptomyces albulus which has resistance to S-(2 amino ethyl)-L- cysteine (called AEC for short), glycin and sulfaguanidine), and carrying out the fermentation production of the epsilon-poly-L-lysine through adding mixed solution of L-lysine, glucose and (NH4)2SO4 in a fed-match mode. The novel method can increase the yield by 25-50 percent than the process of with out the fed batch of L-lysine. The invention changes the method of the original production process, obviously increases the yield of the epsilon-poly-L-lysine, lowers the cost, and can be used for industrial scale fermentation.

Description

Technical field [0001] The invention belongs to the technical field of fermentation engineering, and specifically relates to a new method for increasing the yield of ε-poly-L-lysine produced by a strain of Streptomyces diastatochromogenes or Streptomyces albulus (Streptomyces albulus). It involves the addition of L-lysine during the fermentation process. Background technique [0002] ε-poly-L-lysine is a lysine homopolymer containing 25-30 residues. It is easily soluble in water, but insoluble in organic solvents such as ethyl acetate, ethanol, and ether; it is thermally stable High performance, it still has antibacterial activity after heating at 120℃ for 10min. ε-Poly-L-lysine is a polypeptide with antibacterial effect. It has a broad antibacterial spectrum. Under neutral and slightly acidic conditions, it is resistant to Gram-positive bacteria, Gram-negative bacteria, molds and yeasts. Bacteria have an inhibitory effect, and it also has an inhibitory effect on thermostable b...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12P13/02C12R1/525C12R1/47
Inventor 贾士儒莫治文谭之磊王国良范宝庆王甜李琳周斌
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY