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Fodder premix and special bacterial strain thereof

A premix, feed technology, applied in animal feed, animal feed, bacteria and other directions, can solve the problems of antibiotic endogenous infection or superinfection of livestock and poultry, reduced effect of human medication, spread of various diseases, etc. Economic value and social significance, improved feed conversion rate, low cost effect

Inactive Publication Date: 2010-03-31
XUCHANG XINBOYANG TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The abuse of antibiotics can easily lead to endogenous infection or superinfection of livestock and poultry; the use of antibiotics in feed leads to drug residues in animal products, which directly threatens human health; the transfer of drug resistance from animals to humans reduces the effectiveness of human medication, Various diseases spread; some drugs are excreted through feces, causing environmental pollution

Method used

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  • Fodder premix and special bacterial strain thereof
  • Fodder premix and special bacterial strain thereof
  • Fodder premix and special bacterial strain thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1, the acquisition of bacterial classification

[0020] 1. Acquisition of Bacillus subtilis Bs006 CGMCC No.2434

[0021] 1. Acquisition of strains

[0022] 1. Take one gram of soil from Taichung County, Taiwan, and add 10ml of Trypticase TM Soy Broth (TSB culture solution; each liter contains tryptone 20.0g, dextrose 1.0g, disodium hydrogen phosphate 2.0g, potassium nitrate 1.0g, adjust the pH to 7.0, add 1.5% agar to the solid medium) and shake and mix After uniformity, it is sterilized by conventional pasteurization.

[0023] 2. The TSB culture solution was diluted 10 times to 10 -4 , each serial dilution was incubated at 37°C for 24 hours, and the initial enrichment screening of spore-forming strains was carried out.

[0024] 3. Then take 100 μl from each dilution tube onto a TSA (Trypticase Soy Agar, Difco, Sparks, U.S.A.) plate, spread evenly with an L-shaped glass rod, and culture at 37°C for 24 hours, and then pick a single colony by random select...

Embodiment 2

[0060] Embodiment 2, the influence of premix of the present invention on broiler growth performance

[0061] In this example, 432 1-day-old Arbor Acres (AA) male broiler chicks were tested to clarify the beneficial effect of the premix provided by the present invention.

[0062] Carry out 6 repeated tests in the present embodiment, each repeated test is specifically as follows:

[0063] 1. Preparation of premix

[0064] (1) Expanded fermentation of Bacillus subtilis (Bacillus subtilis) Bs006 CGMCC No.2434

[0065] 1. The first expanded fermentation

[0066] The activated Bacillus subtilis (Bacillus subtilis) Bs006 CGMCC No.2434 was inserted into the following medium A, and fermented at 37° C. for 12 hours.

[0067] The composition of the culture medium A is as follows: each liter of the culture medium contains 3 g of meat extract and 5 g of peptone; it is prepared with distilled water, and the pH is 7.0.

[0068] 2. The second enlarged fermentation

[0069] The fermented ...

Embodiment 3

[0115] Embodiment 3, the impact of the premix of the present invention on the production performance of dairy cows

[0116] In this example, 60 Holstein lactating cows are tested to illustrate the beneficial effect of the premix provided by the present invention.

[0117] 1. Preparation of premix

[0118] (1) Expanded fermentation of Bacillus subtilis (Bacillus subtilis) Bs006 CGMCC No.2434

[0119] 1. The first expanded fermentation

[0120] The activated Bacillus subtilis (Bacillus subtilis) Bs006 CGMCC No.2434 was inserted into the following medium A, and fermented at 37° C. for 12 hours.

[0121] The composition of the culture medium A is as follows: each liter of the culture medium contains 3 g of meat extract and 5 g of peptone; it is prepared with distilled water, and the pH is 7.0.

[0122] 2. The second enlarged fermentation

[0123] The fermented broth from step 1 was introduced into the following medium B with an inoculum size of 10% (volume ratio), and fermente...

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Abstract

The invention discloses a fodder premix and a special bacterial strain thereof; lactobacillus delbrueckii subspecies ST007 with the collection number of CGMCC No.2433 is provided by the invention; inthe fodder premix, active constituents are culture which is obtained by fermenting a substrate with bacillus subtilis Bs006 CGMCC No.2434 and lactobacillus delbrueckii subspecies ST007 CGMCC No.2433;the substrate is at least one of bean pulp, horsebean pulp, rape seed dregs, bean flour and cornmeal. The fodder premix has the characteristics of no toxicity, no drug resistance and no side effect, and can be added in various animal fodders, so as to remarkably promote the growth of animals, improve the fodder conversion ratio and enhance the immunity of the animals. The preparation method of thefodder premix is simple and has low cost, thereby having great economic value and social significance.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a feed premix and special bacterial strains thereof. Background technique [0002] Generally, substances that can be eaten by animals and can give animals one or more nutrients are collectively referred to as feed. As the population increases year by year and human eating habits change, the demand for animal food increases, and the demand for animal feed also continues to increase. According to the estimates of "International Feed", the output of the global feed industry in 2002 reached 600 million 4 million tons. However, the rapid development of animal husbandry and feed industry also brings some side effects, one of which is the abuse of antibiotics. The abuse of antibiotics can easily lead to endogenous infection or superinfection of livestock and poultry; the use of antibiotics in feed leads to drug residues in animal products, which directly threatens human health; the transfer...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K1/14C12R1/225C12R1/125A23K10/12A23K10/30A23K10/37
CPCY02P60/87
Inventor 邱云棕干小英曹君平徐庆霖程景章汪志阳张萍
Owner XUCHANG XINBOYANG TECH
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