Artificial dura mater with bioactivity and preparation method thereof

An artificial dura mater, bioactive technology, applied in the field of biomedicine

Active Publication Date: 2010-04-14
MEDPRIN REGENERATIVE MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] The purpose of the present invention is to overcome the deficiencies of the existing artificial dura mater products, to provide a fully absorbable, good flexibility, elasticity and bio-histocompatibility, prevent brain adhesion, fully degradable absorbable and moderate degradation speed, It can promote the process of dura materization, does not contain virus, has no immune rejection, can be arbitrarily cut according to needs, has low preparation cost, short production cycle, easy storage and transportation, simple operation for clinical application, and a wide range of applications. dura mater

Method used

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  • Artificial dura mater with bioactivity and preparation method thereof
  • Artificial dura mater with bioactivity and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0075] (1) Preparation of electrospinning solution, drug-containing hydrosol solution and cross-linking agent solution:

[0076] For the electrospinning solution, hydrophobic L-polylactic acid and ε-caprolactone are selected, and the ratio of the two is 50:50. As a copolymerized polymer material, the number average molecular weight is 260,000, and it is soluble in hexafluoroisopropanol.

[0077] The cross-linking agent solution is 0.1M calcium chloride solution.

[0078] The hydrosol solution containing cytokines adopts the hemostatic factor alginate solution, and the mass percent concentration of the hemostatic factor in the cytokine alginate solution is 10 ppm.

[0079] Put the prepared 0.1M calcium chloride solution into a cell culture dish with a diameter of 150 mm, and place it on the flat receiver shared by the electrospinning device and the printer. Refit the Hewlett-Packard 550C inkjet printer according to the existing patent report, and fix it directly under the elec...

Embodiment 2

[0097] 1. Preparation of anti-adhesion layer

[0098] (1) Preparation of electrospinning solution, drug-containing hydrosol solution and cross-linking agent solution:

[0099] For the electrospinning solution, hydrophobic L-polylactic acid and ε-caprolactone are selected, and the ratio of the two is 50:50. As a copolymerized polymer material, the number average molecular weight is 260,000, and it is soluble in hexafluoroisopropanol.

[0100] The cross-linking agent solution is 0.1M calcium chloride solution.

[0101] The hydrosol solution containing cytokines adopts the hemostatic factor alginate solution, and the mass percent concentration of the hemostatic factor in the cytokine alginate solution is 10 ppm.

[0102] Put the prepared 0.1M calcium chloride solution into a cell culture dish with a diameter of 150 mm, and place it on the flat receiver shared by the electrospinning device and the printer. Refit the Hewlett-Packard 550C inkjet printer according to the existing p...

Embodiment 3

[0131] Carry out dog animal experiment with the dura mater that embodiment 1 makes:

[0132] There are 5 experimental dogs, weighing 15-20KG, aged 1.5-2 years, male or female. Intramuscular injection of ketamine was used for general anesthesia, and after anesthesia and hair removal, the animals were placed on a special operating table in the abdominal recumbent position. Disinfect with 2% iodine and 75% alcohol. The center of the head of the animal was cut longitudinally. The periosteum was separated with a stripper, the double top skull plate was exposed, and the skull was ground with a high-speed drill, and the double top was used to form a bone window. Use small scissors to cut off a rectangular dura mater with a size of 3 cm × 3 cm on the top of both sides to create a top dura mater defect. Electrocautery was performed on the exposed brain surface to cause six lesions of 1mm×1mm in size. The artificial meninges prepared in Example 1 of the present invention were trimme...

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Abstract

The invention provides an artificial dura mater with bioactivity, which is formed from a nano-bionic scaffold and hydrosol attached on the nano-bionic scaffold, wherein cell factors and / or a medicament are / is coated in the hydrosol; the nano-bionic scaffold at least contains two layers of connected structures, wherein the structure layer facing to the brain is a hydrophobic anti-adhesive electrospinning layer, and the structure layer opposite to the brain is a hydrophilic nano-cytoskeleton layer. The invention also provides a preparation method of the artificial dura mater, which comprises the following steps: preparing an electrospinning solution, a hydrosol solution containing the cell factors and / or the medicament and a cross linker solution; preparing the artificial dura mater through electrostatic spinning and biological printing. By simultaneously utilizing a biological printing technology and an electrostatic spinning technology for preparing the artificial dura mater, the cell factors can be effectively distributed according to the requirements of concentration and positions, a brain membranization process is the fastest and optimal, and meanwhile, the following adverse reaction of the medicament is reduced because the artificial dura mater can be accurately positioned and quantified by using the biological printing technology.

Description

technical field [0001] The invention relates to a biologically active artificial dura mater and a preparation method thereof, belonging to the technical field of biomedicine. Background technique [0002] Dural defects are common in neurosurgery clinical work. Open craniocerebral injuries caused by industry, transportation, war, etc., tumor erosion, congenital meningeal defects and other craniocerebral diseases can cause dural defects. The dural defect needs to be repaired in time to prevent cerebrospinal fluid from overflowing, to prevent the swelling of the brain and the compression of atmospheric pressure, otherwise it will endanger human life. Dural defects can also cause complications such as intracranial infection, brain adhesions, and subcutaneous fluid, often causing diseases such as epilepsy, headache, and brain dysfunction. [0003] Although there are many substitutes for dura mater, the materials for repairing dura mater can be divided into four categories: autol...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61F2/08A61F2/02A61L27/38A61L27/54A61L27/14
Inventor 徐弢袁玉宇
Owner MEDPRIN REGENERATIVE MEDICAL TECH
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