Method for preparing high-purity nisin
A nisin, high-purity technology, applied in the field of preparation of high-purity nisin, can solve the problems of complicated purification process and low purity, and achieve the effect of high product purity, simple process steps and less equipment
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0024] Get 300 grams of Nisin crude product (wet powder obtained by pressure filtration through small plates and frames after salting out, nisin weight percentage is about 7%), add 4 liters of hydrochloric acid (pH is 2) and 2 liters of ethanol after pulverizing, Stir well for 1 hour. Slowly add 400 grams of refined salt, stir and dissolve to make the solution saturated, and let it stand for 6 hours. Centrifuge and separate the phases (2000rpm) for 40 minutes. After centrifugation, the whole mixed solution is divided into four layers, which are light phase (mainly ethanol), nisin layer, heavy phase and impurity layer. Residue (a large number of impurities) and light and heavy Phase, take the nisin layer, the Nisin layer is off-white, add 2 liters of ethanol to stir and mix, then centrifuge the phase separation again (rotating speed is 2000rpm, 40 minutes) to remove the liquid, take the precipitate layer, now the Nisin layer is milky white, as If the color is not milky white, ...
Embodiment 2
[0027] Get 300 grams of Nisin crude product (wet powder obtained by pressure filtration through small plates and frames after salting out, nisin content is about 10%), add 2 liters of hydrochloric acid (pH is 6) and 1 liter of acetic acid after pulverizing, fully stir for 3 Hour. Slowly add 600 grams of refined salt, stir and dissolve to make the solution saturated, and let it stand for 10 hours. Centrifuge and separate the phases (5000rpm) for 10 minutes. After centrifugation, the whole mixed solution is divided into four layers, which are light phase, nisin layer, heavy phase and impurity layer. Add 2 liters of acetic acid to mix well, and then centrifuge again to separate the phases to remove the liquid. The precipitated layer is the Nisin layer. Take the precipitated layer. At this time, the Nisin layer is milky white. If the color is not milky white, repeat the centrifugal phase separation until the color is milky white. Take the precipitate and add 4 liters of acetic ac...
Embodiment 3
[0030] Take 300 grams of Nisin crude product (wet powder obtained by pressure filtration with a small plate frame after salting out, nisin content is about 9%), add 1 liter of hydrochloric acid (pH is 4) after crushing, add 2.5 liters after soaking for 6 hours Hydrochloric acid and 2.5 liters of ethanol, stirred thoroughly for 2 hours. Slowly add 150 grams of refined salt, stir for 2 hours, and let stand for 16 hours. The whole mixed solution is divided into four layers, namely light phase (mainly ethanol), Nisin layer, heavy phase and impurity layer. Take out the light phase and the Nisin layer, and centrifuge to separate the phases (10,000 rpm) for 5 minutes to remove the residue (a large amount of impurities) and the light and heavy phases. After centrifugation, the Nisin layer is off-white. Add 2 liters of ethanol and mix well, then centrifuge again to remove impurities and remove the liquid. The precipitated layer is the Nisin layer. Take the precipitated layer. At this ...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More