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Novel microorganism flora mixture and mixed nutrient medium thereof

A technology of microbial flora and culture medium, applied in microorganisms, microorganism-based methods, biological water/sewage treatment, etc., can solve the problem of poor adaptability, long time required, difficulty in rapid expansion and proliferation of microorganisms, easy degeneration and decline in biological activity. problem, to achieve the effect of improving tolerance and high tolerance

Inactive Publication Date: 2012-07-18
河南亚神环保科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

my country has just begun to apply microorganisms to deal with pollution since the 1920s, and scientific and technological workers have also developed a variety of microbial preparations, such as a composite microbial preparation disclosed in Chinese patent document CN 1189555C, but this type of composite microbial preparation has never been used. It can break through the technical bottlenecks such as the difficulty of rapid expansion and proliferation of microorganisms, the easy degeneration of microorganisms, the decline and loss of biological activity, and it is difficult to realize industrialization and commercialization
However, the effect of using microorganisms to treat sewage depends largely on the number, composition and activity of microorganisms in the treatment system. The existing compound microbial preparations have poor adaptability, and it is difficult to revive, reproduce and form dominant bacterial groups in a short time after entering the water body. Biofilm, and the scope of adaptation is small, it takes a long time; and it is inconvenient in startup, dynamic cultivation and dynamic operation management, the actual operation cost is high, and the treatment effect is not stable

Method used

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  • Novel microorganism flora mixture and mixed nutrient medium thereof
  • Novel microorganism flora mixture and mixed nutrient medium thereof
  • Novel microorganism flora mixture and mixed nutrient medium thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Embodiment 1 laboratory urban domestic sewage treatment test

[0055] The urban domestic sewage used in the test treatment comes from the sewage pipe network of Zhengzhou City, and 0.12kg / m of new microbial flora combination agent is put into the sewage 3 After 15 days, a biofilm will be formed, and the water quality will be clarified. During the protection period, continue to add the microbial flora combination agent at 0.02kg / m 3 . The water quality test results before and after the test treatment are shown in Attached Table 1.

[0056] The production steps of the above-mentioned novel microbial flora composition are as follows:

[0057] (1) By weight percentage, the weight ratio of plant powder (fragrant wood powder, rotten wood powder, peanut shell powder, black sesame shell powder, black bean shell powder, and castor plant shell powder is 1:2:2:4:3:2 ) 70%, minerals (keel powder, weathered stone powder, weathered brick powder, rust powder weight ratio is 2:3:3:5...

Embodiment 2

[0060] Embodiment 2 mixed industrial wastewater treatment test

[0061] Take the mixed industrial wastewater discharged from many factories in the suburbs of Zhengzhou City, and put the new microbial flora composition 0.09kg / m into the wastewater 3 After 20 days, a biofilm was formed, and the water quality was clarified. See attached table 2 for the water quality test results before and after treatment.

[0062] The production steps of the above-mentioned novel microbial flora composition are as follows:

[0063] (1) By weight percentage, the weight ratio of plant powder (fragrant wood powder, rotten wood powder, peanut shell powder, black sesame shell powder, black bean shell powder, and castor plant shell powder is 2: 2: 1: 4: 3: 2 ) 80%, minerals (keel powder, weathered stone powder, weathered brick powder, rust powder weight ratio is 2:3:3:5) 3%, animal meal (shrimp shell powder, crab shell powder, oyster shell powder, human The weight ratio of baking powder and white gl...

Embodiment 3

[0066] Example 3 Xinmi City Xinxin Paper Co., Ltd. Recycled Paper Sewage Treatment Project

[0067] The company produces more than 17,000 tons of container board annually, and the daily wastewater discharge is 1,000-1,500 tons. The COD content of wastewater discharged during the production process is 1,800-2,100 mg / L, BOD 5The average value is as high as 568mg / L. In February 2006, the present invention combined with artificial wetlands was used to carry out advanced treatment of papermaking wastewater. The treatment process is: put bacterial agents in the original wastewater for pretreatment, and then pass through the grid into the coagulation sedimentation tank for precipitation. , and then enter the aeration tank for oxygenation and oxidation, and then add high-efficiency complex microbial flora combination agent for treatment, and finally the supernatant is introduced into the constructed wetland for treatment. It took 40 days from the test water inflow to the effluent reac...

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Abstract

The invention relates to a novel microorganism flora mixture and a mixed nutrient medium thereof. The nutrient medium is filter liquor which is prepared by taking plant powder, mineral composition, animal powder, organic acid and the like as raw materials, adding sewage or comprehensive sewage in cities to be processed, mixing, fermenting and filtering. The microorganism flora mixture is a microorganism preparation which is prepared by the following steps: respectively selecting fusarium oxysporum, aspergillus fumigatus, sphaerotilus, vorticella microstoma, photosynthetic bacteria, methanebacteria, desulphovibrio, nitrobacterium, pseudomonas fluorescens and other bacterial strains from obligate aerobes group, anaerobic bacteria group and facultative aerobe group preferentially, then expanding and cultivating, inoculating into the above mixed nutrient medium, mixing, cultivating and domesticating for 15-30 days at the temperature of 20-45 DEG C. The mixed nutrient medium in the invention can cultivate, propagate and domesticate flora mixture with the combination advantages of flora; the flora has stable integrity, no easy variation and strong adaptability, and the invention breaks through the technical bottlenecks of easy degeneracy of microorganism flora, easy decrease of biological activity and slow proliferation and expansion.

Description

technical field [0001] The invention relates to the field of sewage treatment, in particular to a novel microbial flora combination agent and a culture medium thereof. Background technique [0002] At present, the problem of environmental pollution in our country is becoming more and more prominent, such as water pollution control is plaguing governments at all levels and various production enterprises. As we all know, although some pollutants can be removed by physical methods (such as salvage), it is difficult to treat chemical pollutants such as ammonia nitrogen, nitrite, and poultry manure, etc. Chemical methods are easy to cause secondary pollution, and It is difficult to carry out advanced treatment of sewage by expensive physical and chemical treatment methods. At present, more than 90% of the environmental treatment projects in our country adopt methods such as activated sludge method, oxidation ditch and carbonization method. These methods are more complex, requir...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/16C12N1/14C02F3/34C12R1/645C12R1/01C12R1/07C12R1/64C12R1/39C12R1/145C12R1/66C12R1/77
Inventor 王培名
Owner 河南亚神环保科技有限公司
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