Preparation method of high-efficiency compound microorganism soybean meal leaven

A technology of compound microorganisms and starters, applied in animal feed, animal feed, applications, etc., can solve the problems of uneven activity of starters, difficult to predict fermentation, slow growth, etc., to reduce cholesterol levels, improve protein utilization, and vitality uniform effect

Inactive Publication Date: 2010-06-02
四川渴望生物科技有限公司
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  • Abstract
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  • Application Information

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Problems solved by technology

These starters have uneven vigor, slow growth, and are often contaminated by other microorganisms, resulting in unpredictable fermentations and even complete fermentation failures
Therefore, before fermentatio

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] The preparation method of high-efficiency composite microbial soybean meal starter of the present invention comprises the following steps:

[0037] 1) Strain optimization screening:

[0038] The strains of Lactobacillus acidophilus, Lactobacillus lactis, Saccharomyces cerevisiae, Candida utilis, Bacillus subtilis, and Bacillus licheniformis were acid-resistant, bile-salt-resistant, high-copper-resistant and temperature-resistant respectively;

[0039] ①Acid-resistant domestication of strains: pH 3.5, acid-resistant strains were screened out;

[0040] ② Cholic acid resistance acclimatization: add different concentrations of cholate to the culture medium to acclimatize the strain;

[0041] ③Breeding for high copper resistance: grow in MRS broth medium containing 250mg / kg copper;

[0042] ④Temperature-resistant acclimatization: Inoculate the activated bacterial culture into LB and TB liquid medium, heat it in a water bath at 40, 45, 50, 55, and 60°C for a certain period ...

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Abstract

The invention relates to a preparation method of a high-efficiency compound microorganism soybean meal leaven, belonging to the technical field of biological enzymolysis and fermentation. The preparation method comprises the following steps of: respectively carrying out optimal screening on lactobacillus acidophilus, lactobacillus lactis, saccharomyces cerevisiae, Candida utilis, bacillus subtilis and bacillus licheniformis; mixing and culturing by shaking a bottle; culturing by using a 10L fermentation tank; manufacturing a microorganism compound protective agent; spray drying in low temperature; preparing the soybean meal leaven and the like. The high-efficiency compound microorganism soybean meal leaven can efficiently inhibit the growth of mixed bacterium and eliminate or remarkably lower anti-nutritional factors and flatulent factors in soybean meal, enhance the protein utilization ratio and strengthen the immunity and has the excellent characteristics of acid resistance, salt resistance, temperature resistance and bile resistance. The invention has the advantages of simple preparation method, low cost, stable and reliable produced product quality and wide application range.

Description

technical field [0001] The invention relates to a method for preparing a microbial starter, in particular to a method for preparing a high-efficiency composite microbial soybean meal starter, and belongs to the technical field of biological enzymolysis and fermentation. Background technique [0002] Soybean meal is a by-product of soybean oil extraction. Its crude protein content is as high as 40%-50%. It is rich in nutrition, low in cost, and has a reasonable composition of protein and amino acids. It is widely used as animal feed. However, soybean meal contains a large amount of anti-nutritional factors, which not only affects the utilization efficiency of protein, but also causes intestinal damage in young animals, resulting in post-weaning diarrhea in young animals, which significantly affects the health and survival of animals. The solution is to add soybean meal starter to the soybean meal to eliminate or reduce the anti-nutritional factors in the soybean meal. There ...

Claims

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Application Information

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IPC IPC(8): A23K1/16A23K1/14A23K10/18
Inventor 冯建国
Owner 四川渴望生物科技有限公司
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